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Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes
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| Zeitschriftentitel: | BioFactors |
|---|---|
| Personen und Körperschaften: | , |
| In: | BioFactors, 36, 2010, 3, S. 222-228 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Schmelzer, Constance Döring, Frank Schmelzer, Constance Döring, Frank |
|---|---|
| author |
Schmelzer, Constance Döring, Frank |
| spellingShingle |
Schmelzer, Constance Döring, Frank BioFactors Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes Clinical Biochemistry Molecular Medicine General Medicine Biochemistry |
| author_sort |
schmelzer, constance |
| spelling |
Schmelzer, Constance Döring, Frank 0951-6433 1872-8081 Wiley Clinical Biochemistry Molecular Medicine General Medicine Biochemistry http://dx.doi.org/10.1002/biof.93 <jats:title>Abstract</jats:title><jats:p>Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub>) is an obligatory element in the respiratory chain and functions as a potent antioxidant of lipid membranes. More recently, anti‐inflammatory effects as well as an impact of CoQ<jats:sub>10</jats:sub> on gene expression have been observed. To reveal putative effects of Q<jats:sub>10</jats:sub> on LPS‐induced gene expression, whole genome expression analysis was performed in the monocytic cell line THP‐1. Thousand one hundred twenty‐nine and 710 probe sets have been identified to be significantly (<jats:italic>P</jats:italic> ≤ 0.05) up and downregulated in LPS‐treated cells when compared with controls, respectively. Text mining analysis of the top 50 LPS upregulated genes revealed a functional connection in the NFκB pathway and confirmed our applied <jats:italic>in vitro</jats:italic> stimulation model. Moreover, 33 LPS‐sensitive genes have been identified to be significantly downregulated by Q<jats:sub>10</jats:sub>‐treatment between a factor of 1.32 and 1.85. GeneOntology (GO) analysis revealed for the Q<jats:sub>10</jats:sub>‐sensitve genes a primary involvement in protein metabolism (<jats:italic>e.g.</jats:italic>, HERC1 and EPS15), cell proliferation (<jats:italic>e.g.</jats:italic>, CCDC100 and SMURF1), and transcriptional processes (<jats:italic>e.g.</jats:italic>, CNOT4 and STK4). Three genes were either related to NFκB transcription factor activity (ERC1), cytokinesis (DIAPH2), or modulation of oxidative stress (MSRA). In conclusion, our data provide evidence that Q<jats:sub>10</jats:sub> downregulates LPS‐inducible genes in the monocytic cell line THP‐1. Thus, the previously described effects of Q<jats:sub>10</jats:sub> on the reduction of proinflammatory mediators might be due to its antioxidant impact on gene expression.</jats:p> Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes BioFactors |
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10.1002/biof.93 |
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| title |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_unstemmed |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_full |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_fullStr |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_full_unstemmed |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_short |
Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_sort |
identification of lps‐inducible genes downregulated by ubiquinone in human thp‐1 monocytes |
| topic |
Clinical Biochemistry Molecular Medicine General Medicine Biochemistry |
| url |
http://dx.doi.org/10.1002/biof.93 |
| publishDate |
2010 |
| physical |
222-228 |
| description |
<jats:title>Abstract</jats:title><jats:p>Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub>) is an obligatory element in the respiratory chain and functions as a potent antioxidant of lipid membranes. More recently, anti‐inflammatory effects as well as an impact of CoQ<jats:sub>10</jats:sub> on gene expression have been observed. To reveal putative effects of Q<jats:sub>10</jats:sub> on LPS‐induced gene expression, whole genome expression analysis was performed in the monocytic cell line THP‐1. Thousand one hundred twenty‐nine and 710 probe sets have been identified to be significantly (<jats:italic>P</jats:italic> ≤ 0.05) up and downregulated in LPS‐treated cells when compared with controls, respectively. Text mining analysis of the top 50 LPS upregulated genes revealed a functional connection in the NFκB pathway and confirmed our applied <jats:italic>in vitro</jats:italic> stimulation model. Moreover, 33 LPS‐sensitive genes have been identified to be significantly downregulated by Q<jats:sub>10</jats:sub>‐treatment between a factor of 1.32 and 1.85. GeneOntology (GO) analysis revealed for the Q<jats:sub>10</jats:sub>‐sensitve genes a primary involvement in protein metabolism (<jats:italic>e.g.</jats:italic>, HERC1 and EPS15), cell proliferation (<jats:italic>e.g.</jats:italic>, CCDC100 and SMURF1), and transcriptional processes (<jats:italic>e.g.</jats:italic>, CNOT4 and STK4). Three genes were either related to NFκB transcription factor activity (ERC1), cytokinesis (DIAPH2), or modulation of oxidative stress (MSRA). In conclusion, our data provide evidence that Q<jats:sub>10</jats:sub> downregulates LPS‐inducible genes in the monocytic cell line THP‐1. Thus, the previously described effects of Q<jats:sub>10</jats:sub> on the reduction of proinflammatory mediators might be due to its antioxidant impact on gene expression.</jats:p> |
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| author | Schmelzer, Constance, Döring, Frank |
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| author_sort | schmelzer, constance |
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| description | <jats:title>Abstract</jats:title><jats:p>Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub>) is an obligatory element in the respiratory chain and functions as a potent antioxidant of lipid membranes. More recently, anti‐inflammatory effects as well as an impact of CoQ<jats:sub>10</jats:sub> on gene expression have been observed. To reveal putative effects of Q<jats:sub>10</jats:sub> on LPS‐induced gene expression, whole genome expression analysis was performed in the monocytic cell line THP‐1. Thousand one hundred twenty‐nine and 710 probe sets have been identified to be significantly (<jats:italic>P</jats:italic> ≤ 0.05) up and downregulated in LPS‐treated cells when compared with controls, respectively. Text mining analysis of the top 50 LPS upregulated genes revealed a functional connection in the NFκB pathway and confirmed our applied <jats:italic>in vitro</jats:italic> stimulation model. Moreover, 33 LPS‐sensitive genes have been identified to be significantly downregulated by Q<jats:sub>10</jats:sub>‐treatment between a factor of 1.32 and 1.85. GeneOntology (GO) analysis revealed for the Q<jats:sub>10</jats:sub>‐sensitve genes a primary involvement in protein metabolism (<jats:italic>e.g.</jats:italic>, HERC1 and EPS15), cell proliferation (<jats:italic>e.g.</jats:italic>, CCDC100 and SMURF1), and transcriptional processes (<jats:italic>e.g.</jats:italic>, CNOT4 and STK4). Three genes were either related to NFκB transcription factor activity (ERC1), cytokinesis (DIAPH2), or modulation of oxidative stress (MSRA). In conclusion, our data provide evidence that Q<jats:sub>10</jats:sub> downregulates LPS‐inducible genes in the monocytic cell line THP‐1. Thus, the previously described effects of Q<jats:sub>10</jats:sub> on the reduction of proinflammatory mediators might be due to its antioxidant impact on gene expression.</jats:p> |
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| spelling | Schmelzer, Constance Döring, Frank 0951-6433 1872-8081 Wiley Clinical Biochemistry Molecular Medicine General Medicine Biochemistry http://dx.doi.org/10.1002/biof.93 <jats:title>Abstract</jats:title><jats:p>Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub>) is an obligatory element in the respiratory chain and functions as a potent antioxidant of lipid membranes. More recently, anti‐inflammatory effects as well as an impact of CoQ<jats:sub>10</jats:sub> on gene expression have been observed. To reveal putative effects of Q<jats:sub>10</jats:sub> on LPS‐induced gene expression, whole genome expression analysis was performed in the monocytic cell line THP‐1. Thousand one hundred twenty‐nine and 710 probe sets have been identified to be significantly (<jats:italic>P</jats:italic> ≤ 0.05) up and downregulated in LPS‐treated cells when compared with controls, respectively. Text mining analysis of the top 50 LPS upregulated genes revealed a functional connection in the NFκB pathway and confirmed our applied <jats:italic>in vitro</jats:italic> stimulation model. Moreover, 33 LPS‐sensitive genes have been identified to be significantly downregulated by Q<jats:sub>10</jats:sub>‐treatment between a factor of 1.32 and 1.85. GeneOntology (GO) analysis revealed for the Q<jats:sub>10</jats:sub>‐sensitve genes a primary involvement in protein metabolism (<jats:italic>e.g.</jats:italic>, HERC1 and EPS15), cell proliferation (<jats:italic>e.g.</jats:italic>, CCDC100 and SMURF1), and transcriptional processes (<jats:italic>e.g.</jats:italic>, CNOT4 and STK4). Three genes were either related to NFκB transcription factor activity (ERC1), cytokinesis (DIAPH2), or modulation of oxidative stress (MSRA). In conclusion, our data provide evidence that Q<jats:sub>10</jats:sub> downregulates LPS‐inducible genes in the monocytic cell line THP‐1. Thus, the previously described effects of Q<jats:sub>10</jats:sub> on the reduction of proinflammatory mediators might be due to its antioxidant impact on gene expression.</jats:p> Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes BioFactors |
| spellingShingle | Schmelzer, Constance, Döring, Frank, BioFactors, Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes, Clinical Biochemistry, Molecular Medicine, General Medicine, Biochemistry |
| title | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_full | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_fullStr | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_full_unstemmed | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_short | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| title_sort | identification of lps‐inducible genes downregulated by ubiquinone in human thp‐1 monocytes |
| title_unstemmed | Identification of LPS‐inducible genes downregulated by ubiquinone in human THP‐1 monocytes |
| topic | Clinical Biochemistry, Molecular Medicine, General Medicine, Biochemistry |
| url | http://dx.doi.org/10.1002/biof.93 |