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Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines
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| Zeitschriftentitel: | BioFactors |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | BioFactors, 31, 2007, 1, S. 35-41 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank |
|---|---|
| author |
Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank |
| spellingShingle |
Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank BioFactors Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines Clinical Biochemistry Molecular Medicine General Medicine Biochemistry |
| author_sort |
schmelzer, constance |
| spelling |
Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank 0951-6433 1872-8081 Wiley Clinical Biochemistry Molecular Medicine General Medicine Biochemistry http://dx.doi.org/10.1002/biof.5520310104 <jats:title>Abstract</jats:title><jats:p>Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub> ) functions as an anti‐inflammatory molecule. Here we studied the influence of CoQ<jats:sub>10</jats:sub> (Kaneka QTM ) on secretion of the pro‐inflammatory 10 cytokine tumor necrosis factor‐alpha (TNF‐α) by using the human and murine monocytic cell lines TH P‐1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro‐inflammatory apoE4. Incubation of cells with physiological (0.1–10 μM) and supra‐physiological (> 10 to ≤ 100 μM) concentrations of CoQ<jats:sub>10</jats:sub> led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF‐α. When THP‐1 cells were pre‐incubated with 10 μM CoQ<jats:sub>10</jats:sub>, the LPS‐induced TNF‐α release was significantly decreased to 72 ± 32%. This effect is similar to those obtained by 10 μM N‐Acetyl‐Cysteine, a well known reference antioxidant. In RAW264.7‐apoE3 and ‐apoE4 cells, significant reductions of LPS‐induced TNF‐α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found with 2.5 μM and 75 μM CoQ<jats:sub>10</jats:sub>, respectively. In conclusion, CoQ<jats:sub>10</jats:sub> has moderate anti‐inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.</jats:p> Effects of Coenzyme Q<sub>10</sub> on TNF‐α secretion in human and murine monocytic cell lines BioFactors |
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10.1002/biof.5520310104 |
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| title |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_unstemmed |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_full |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_fullStr |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_full_unstemmed |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_short |
Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_sort |
effects of coenzyme q<sub>10</sub> on tnf‐α secretion in human and murine monocytic cell lines |
| topic |
Clinical Biochemistry Molecular Medicine General Medicine Biochemistry |
| url |
http://dx.doi.org/10.1002/biof.5520310104 |
| publishDate |
2007 |
| physical |
35-41 |
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<jats:title>Abstract</jats:title><jats:p>Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub> ) functions as an anti‐inflammatory molecule. Here we studied the influence of CoQ<jats:sub>10</jats:sub> (Kaneka QTM ) on secretion of the pro‐inflammatory 10 cytokine tumor necrosis factor‐alpha (TNF‐α) by using the human and murine monocytic cell lines TH P‐1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro‐inflammatory apoE4. Incubation of cells with physiological (0.1–10 μM) and supra‐physiological (> 10 to ≤ 100 μM) concentrations of CoQ<jats:sub>10</jats:sub> led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF‐α. When THP‐1 cells were pre‐incubated with 10 μM CoQ<jats:sub>10</jats:sub>, the LPS‐induced TNF‐α release was significantly decreased to 72 ± 32%. This effect is similar to those obtained by 10 μM N‐Acetyl‐Cysteine, a well known reference antioxidant. In RAW264.7‐apoE3 and ‐apoE4 cells, significant reductions of LPS‐induced TNF‐α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found with 2.5 μM and 75 μM CoQ<jats:sub>10</jats:sub>, respectively. In conclusion, CoQ<jats:sub>10</jats:sub> has moderate anti‐inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.</jats:p> |
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| author | Schmelzer, Constance, Lorenz, Gerti, Lindner, Inka, Rimbach, Gerald, Niklowitz, Petra, Menke, Thomas, Döring, Frank |
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| description | <jats:title>Abstract</jats:title><jats:p>Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub> ) functions as an anti‐inflammatory molecule. Here we studied the influence of CoQ<jats:sub>10</jats:sub> (Kaneka QTM ) on secretion of the pro‐inflammatory 10 cytokine tumor necrosis factor‐alpha (TNF‐α) by using the human and murine monocytic cell lines TH P‐1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro‐inflammatory apoE4. Incubation of cells with physiological (0.1–10 μM) and supra‐physiological (> 10 to ≤ 100 μM) concentrations of CoQ<jats:sub>10</jats:sub> led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF‐α. When THP‐1 cells were pre‐incubated with 10 μM CoQ<jats:sub>10</jats:sub>, the LPS‐induced TNF‐α release was significantly decreased to 72 ± 32%. This effect is similar to those obtained by 10 μM N‐Acetyl‐Cysteine, a well known reference antioxidant. In RAW264.7‐apoE3 and ‐apoE4 cells, significant reductions of LPS‐induced TNF‐α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found with 2.5 μM and 75 μM CoQ<jats:sub>10</jats:sub>, respectively. In conclusion, CoQ<jats:sub>10</jats:sub> has moderate anti‐inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.</jats:p> |
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| spelling | Schmelzer, Constance Lorenz, Gerti Lindner, Inka Rimbach, Gerald Niklowitz, Petra Menke, Thomas Döring, Frank 0951-6433 1872-8081 Wiley Clinical Biochemistry Molecular Medicine General Medicine Biochemistry http://dx.doi.org/10.1002/biof.5520310104 <jats:title>Abstract</jats:title><jats:p>Studies in humans and cell culture as well as bioinformatics suggested that Coenzyme Q<jats:sub>10</jats:sub> (CoQ<jats:sub>10</jats:sub> ) functions as an anti‐inflammatory molecule. Here we studied the influence of CoQ<jats:sub>10</jats:sub> (Kaneka QTM ) on secretion of the pro‐inflammatory 10 cytokine tumor necrosis factor‐alpha (TNF‐α) by using the human and murine monocytic cell lines TH P‐1 and RAW264.7 expressing human apolipoprotein E3 (apoE3) or pro‐inflammatory apoE4. Incubation of cells with physiological (0.1–10 μM) and supra‐physiological (> 10 to ≤ 100 μM) concentrations of CoQ<jats:sub>10</jats:sub> led to an intracellular accumulation of its reduced form without any cytotoxic effects. Stimulation of cell models with lipopolysaccharide (LPS) resulted in a substantially release of TNF‐α. When THP‐1 cells were pre‐incubated with 10 μM CoQ<jats:sub>10</jats:sub>, the LPS‐induced TNF‐α release was significantly decreased to 72 ± 32%. This effect is similar to those obtained by 10 μM N‐Acetyl‐Cysteine, a well known reference antioxidant. In RAW264.7‐apoE3 and ‐apoE4 cells, significant reductions of LPS‐induced TNF‐α secretion to 73.3 ± 2.8% and 74.7 ± 8.9% were found with 2.5 μM and 75 μM CoQ<jats:sub>10</jats:sub>, respectively. In conclusion, CoQ<jats:sub>10</jats:sub> has moderate anti‐inflammatory effects in two monocytic cell lines which could be mediated by its antioxidant activity.</jats:p> Effects of Coenzyme Q<sub>10</sub> on TNF‐α secretion in human and murine monocytic cell lines BioFactors |
| spellingShingle | Schmelzer, Constance, Lorenz, Gerti, Lindner, Inka, Rimbach, Gerald, Niklowitz, Petra, Menke, Thomas, Döring, Frank, BioFactors, Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines, Clinical Biochemistry, Molecular Medicine, General Medicine, Biochemistry |
| title | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_full | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_fullStr | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_full_unstemmed | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_short | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| title_sort | effects of coenzyme q<sub>10</sub> on tnf‐α secretion in human and murine monocytic cell lines |
| title_unstemmed | Effects of Coenzyme Q10 on TNF‐α secretion in human and murine monocytic cell lines |
| topic | Clinical Biochemistry, Molecular Medicine, General Medicine, Biochemistry |
| url | http://dx.doi.org/10.1002/biof.5520310104 |