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Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and pr...
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| Zeitschriftentitel: | Arthritis & Rheumatism |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Arthritis & Rheumatism, 37, 1994, 10, S. 1468-1478 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. |
|---|---|
| author |
Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. |
| spellingShingle |
Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. Arthritis & Rheumatism Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution Pharmacology (medical) Immunology Rheumatology Immunology and Allergy |
| author_sort |
williams, william v. |
| spelling |
Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. 0004-3591 1529-0131 Wiley Pharmacology (medical) Immunology Rheumatology Immunology and Allergy http://dx.doi.org/10.1002/art.1780371010 <jats:title>Abstract</jats:title><jats:p><jats:italic>Objective</jats:italic>. To analyze the molecular and functional characteristics of a soluble form of the granulocytemacrophage colony‐stimulating factor receptor α chain (sGM‐CSFRα), and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue.</jats:p><jats:p><jats:italic>Methods</jats:italic>. We amplified, cloned, and expressed the sGM‐CSFRα and transmembrane form of the receptor (tmGM‐CSFRα) from complementary DNA derived from a human myelomonocytic cell line. Competitive polymerase chain reaction assays were developed to determine the absolute and relative amounts of tmGM‐CSFRα versus sGM‐CSFRα message synthesized by various cell lines and tissues.S</jats:p><jats:p><jats:italic>Results</jats:italic>. sGM‐CSFRα transcripts were detected in bone marrow, monocyte/macrophages (cultured in GM‐CSF), rheumatoid synovial tissue, and rheumatoid synovial tissue T cell lines, and represented the predominant transcript in synovial fibroblasts and osteoarthritis synovial tissue. Levels of expression in monocyte/macrophages and some synovial fibroblast and T cell lines approached those seen in transfected cell lines producing functional sGM‐CSFRα.</jats:p><jats:p><jats:italic>Conclusion</jats:italic>. sGM‐CSFRα represents a functional antagonist of GM‐CSF activity in vitro. Expression of sGM‐CSFRα in bone marrow, rheumatoid synovial tissue T cells, and synovial fibroblasts suggests an important role in vivo, both in regulating myelopoiesis and in modulating the immune response.</jats:p> Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution Arthritis & Rheumatism |
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Wiley, 1994 |
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| title |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_unstemmed |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_full |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_fullStr |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_full_unstemmed |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_short |
Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_sort |
molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| topic |
Pharmacology (medical) Immunology Rheumatology Immunology and Allergy |
| url |
http://dx.doi.org/10.1002/art.1780371010 |
| publishDate |
1994 |
| physical |
1468-1478 |
| description |
<jats:title>Abstract</jats:title><jats:p><jats:italic>Objective</jats:italic>. To analyze the molecular and functional characteristics of a soluble form of the granulocytemacrophage colony‐stimulating factor receptor α chain (sGM‐CSFRα), and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue.</jats:p><jats:p><jats:italic>Methods</jats:italic>. We amplified, cloned, and expressed the sGM‐CSFRα and transmembrane form of the receptor (tmGM‐CSFRα) from complementary DNA derived from a human myelomonocytic cell line. Competitive polymerase chain reaction assays were developed to determine the absolute and relative amounts of tmGM‐CSFRα versus sGM‐CSFRα message synthesized by various cell lines and tissues.S</jats:p><jats:p><jats:italic>Results</jats:italic>. sGM‐CSFRα transcripts were detected in bone marrow, monocyte/macrophages (cultured in GM‐CSF), rheumatoid synovial tissue, and rheumatoid synovial tissue T cell lines, and represented the predominant transcript in synovial fibroblasts and osteoarthritis synovial tissue. Levels of expression in monocyte/macrophages and some synovial fibroblast and T cell lines approached those seen in transfected cell lines producing functional sGM‐CSFRα.</jats:p><jats:p><jats:italic>Conclusion</jats:italic>. sGM‐CSFRα represents a functional antagonist of GM‐CSF activity in vitro. Expression of sGM‐CSFRα in bone marrow, rheumatoid synovial tissue T cells, and synovial fibroblasts suggests an important role in vivo, both in regulating myelopoiesis and in modulating the immune response.</jats:p> |
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| author | Williams, William V., Vonfeldt, Joan M., Rosenbaum, Helga, Ugen, Kenneth E., Weiner, David B. |
| author_facet | Williams, William V., Vonfeldt, Joan M., Rosenbaum, Helga, Ugen, Kenneth E., Weiner, David B., Williams, William V., Vonfeldt, Joan M., Rosenbaum, Helga, Ugen, Kenneth E., Weiner, David B. |
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| description | <jats:title>Abstract</jats:title><jats:p><jats:italic>Objective</jats:italic>. To analyze the molecular and functional characteristics of a soluble form of the granulocytemacrophage colony‐stimulating factor receptor α chain (sGM‐CSFRα), and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue.</jats:p><jats:p><jats:italic>Methods</jats:italic>. We amplified, cloned, and expressed the sGM‐CSFRα and transmembrane form of the receptor (tmGM‐CSFRα) from complementary DNA derived from a human myelomonocytic cell line. Competitive polymerase chain reaction assays were developed to determine the absolute and relative amounts of tmGM‐CSFRα versus sGM‐CSFRα message synthesized by various cell lines and tissues.S</jats:p><jats:p><jats:italic>Results</jats:italic>. sGM‐CSFRα transcripts were detected in bone marrow, monocyte/macrophages (cultured in GM‐CSF), rheumatoid synovial tissue, and rheumatoid synovial tissue T cell lines, and represented the predominant transcript in synovial fibroblasts and osteoarthritis synovial tissue. Levels of expression in monocyte/macrophages and some synovial fibroblast and T cell lines approached those seen in transfected cell lines producing functional sGM‐CSFRα.</jats:p><jats:p><jats:italic>Conclusion</jats:italic>. sGM‐CSFRα represents a functional antagonist of GM‐CSF activity in vitro. Expression of sGM‐CSFRα in bone marrow, rheumatoid synovial tissue T cells, and synovial fibroblasts suggests an important role in vivo, both in regulating myelopoiesis and in modulating the immune response.</jats:p> |
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| imprint | Wiley, 1994 |
| imprint_str_mv | Wiley, 1994 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11 |
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| physical | 1468-1478 |
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| series | Arthritis & Rheumatism |
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| spelling | Williams, William V. Vonfeldt, Joan M. Rosenbaum, Helga Ugen, Kenneth E. Weiner, David B. 0004-3591 1529-0131 Wiley Pharmacology (medical) Immunology Rheumatology Immunology and Allergy http://dx.doi.org/10.1002/art.1780371010 <jats:title>Abstract</jats:title><jats:p><jats:italic>Objective</jats:italic>. To analyze the molecular and functional characteristics of a soluble form of the granulocytemacrophage colony‐stimulating factor receptor α chain (sGM‐CSFRα), and analyze transcript expression in immune cells and the cellular constituents of rheumatoid arthritis synovial tissue.</jats:p><jats:p><jats:italic>Methods</jats:italic>. We amplified, cloned, and expressed the sGM‐CSFRα and transmembrane form of the receptor (tmGM‐CSFRα) from complementary DNA derived from a human myelomonocytic cell line. Competitive polymerase chain reaction assays were developed to determine the absolute and relative amounts of tmGM‐CSFRα versus sGM‐CSFRα message synthesized by various cell lines and tissues.S</jats:p><jats:p><jats:italic>Results</jats:italic>. sGM‐CSFRα transcripts were detected in bone marrow, monocyte/macrophages (cultured in GM‐CSF), rheumatoid synovial tissue, and rheumatoid synovial tissue T cell lines, and represented the predominant transcript in synovial fibroblasts and osteoarthritis synovial tissue. Levels of expression in monocyte/macrophages and some synovial fibroblast and T cell lines approached those seen in transfected cell lines producing functional sGM‐CSFRα.</jats:p><jats:p><jats:italic>Conclusion</jats:italic>. sGM‐CSFRα represents a functional antagonist of GM‐CSF activity in vitro. Expression of sGM‐CSFRα in bone marrow, rheumatoid synovial tissue T cells, and synovial fibroblasts suggests an important role in vivo, both in regulating myelopoiesis and in modulating the immune response.</jats:p> Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution Arthritis & Rheumatism |
| spellingShingle | Williams, William V., Vonfeldt, Joan M., Rosenbaum, Helga, Ugen, Kenneth E., Weiner, David B., Arthritis & Rheumatism, Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution, Pharmacology (medical), Immunology, Rheumatology, Immunology and Allergy |
| title | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_full | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_fullStr | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_full_unstemmed | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_short | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_sort | molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| title_unstemmed | Molecular cloning of a soluble form of the granulocyte‐‐macrophage colony‐stimulating factor receptor α chain from a myelomonocytic cell line. expression, biologic activity, and preliminary analysis of transcript distribution |
| topic | Pharmacology (medical), Immunology, Rheumatology, Immunology and Allergy |
| url | http://dx.doi.org/10.1002/art.1780371010 |