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Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing
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| Zeitschriftentitel: | Applications in Plant Sciences |
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| Personen und Körperschaften: | , , , , , , |
| In: | Applications in Plant Sciences, 6, 2018, 4 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos |
|---|---|
| author |
Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos |
| spellingShingle |
Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos Applications in Plant Sciences Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing Plant Science Ecology, Evolution, Behavior and Systematics |
| author_sort |
zenk, fides lea |
| spelling |
Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos 2168-0450 2168-0450 Wiley Plant Science Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1002/aps3.1147 <jats:sec><jats:title>Premise of the Study</jats:title><jats:p>Microsatellite markers were developed in <jats:italic>Deuterocohnia longipetala</jats:italic> (Bromeliaceae) to investigate species and subspecies boundaries within the genus and the genetic diversity of natural populations.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>We used 454 pyrosequencing to isolate 835 microsatellite loci in <jats:italic>D. longipetala</jats:italic>. Of 64 loci selected for primer design, 15 were polymorphic among 23 individuals of <jats:italic>D. longipetala</jats:italic> and 76 individuals of the heterologous subspecies <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>meziana</jats:italic> and <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>carmineo‐viridiflora</jats:italic>. Twelve and 13 of these loci were also polymorphic in one population each of <jats:italic>D. brevispicata</jats:italic> and <jats:italic>D. seramisiana</jats:italic>, respectively. Numbers of alleles per locus varied from two to 14 in <jats:italic>D. longipetala</jats:italic>, two to 12 in <jats:italic>D. meziana</jats:italic>, one to nine in <jats:italic>D. brevispicata</jats:italic>, and one to 10 in <jats:italic>D. seramisiana</jats:italic>. <jats:styled-content style="fixed-case">STRUCTURE</jats:styled-content> analyses clearly separated the taxa from each other.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 15 new microsatellite markers are promising tools for studying population genetics in <jats:italic>Deuterocohnia</jats:italic> species.</jats:p></jats:sec> Development of 15 nuclear microsatellite markers in <i>Deuterocohnia</i> (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing Applications in Plant Sciences |
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Wiley, 2018 |
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Wiley, 2018 |
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| title |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_unstemmed |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_full |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_fullStr |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_full_unstemmed |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_short |
Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_sort |
development of 15 nuclear microsatellite markers in <i>deuterocohnia</i> (pitcairnioideae; bromeliaceae) using 454 pyrosequencing |
| topic |
Plant Science Ecology, Evolution, Behavior and Systematics |
| url |
http://dx.doi.org/10.1002/aps3.1147 |
| publishDate |
2018 |
| physical |
|
| description |
<jats:sec><jats:title>Premise of the Study</jats:title><jats:p>Microsatellite markers were developed in <jats:italic>Deuterocohnia longipetala</jats:italic> (Bromeliaceae) to investigate species and subspecies boundaries within the genus and the genetic diversity of natural populations.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>We used 454 pyrosequencing to isolate 835 microsatellite loci in <jats:italic>D. longipetala</jats:italic>. Of 64 loci selected for primer design, 15 were polymorphic among 23 individuals of <jats:italic>D. longipetala</jats:italic> and 76 individuals of the heterologous subspecies <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>meziana</jats:italic> and <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>carmineo‐viridiflora</jats:italic>. Twelve and 13 of these loci were also polymorphic in one population each of <jats:italic>D. brevispicata</jats:italic> and <jats:italic>D. seramisiana</jats:italic>, respectively. Numbers of alleles per locus varied from two to 14 in <jats:italic>D. longipetala</jats:italic>, two to 12 in <jats:italic>D. meziana</jats:italic>, one to nine in <jats:italic>D. brevispicata</jats:italic>, and one to 10 in <jats:italic>D. seramisiana</jats:italic>. <jats:styled-content style="fixed-case">STRUCTURE</jats:styled-content> analyses clearly separated the taxa from each other.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 15 new microsatellite markers are promising tools for studying population genetics in <jats:italic>Deuterocohnia</jats:italic> species.</jats:p></jats:sec> |
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| author | Zenk, Fides Lea, Firmer, Cynthia, Wöhrmann, Tina, Silva, Luciana Vicente da, Weising, Kurt, Huettel, Bruno, Paggi, Gecele Matos |
| author_facet | Zenk, Fides Lea, Firmer, Cynthia, Wöhrmann, Tina, Silva, Luciana Vicente da, Weising, Kurt, Huettel, Bruno, Paggi, Gecele Matos, Zenk, Fides Lea, Firmer, Cynthia, Wöhrmann, Tina, Silva, Luciana Vicente da, Weising, Kurt, Huettel, Bruno, Paggi, Gecele Matos |
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| description | <jats:sec><jats:title>Premise of the Study</jats:title><jats:p>Microsatellite markers were developed in <jats:italic>Deuterocohnia longipetala</jats:italic> (Bromeliaceae) to investigate species and subspecies boundaries within the genus and the genetic diversity of natural populations.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>We used 454 pyrosequencing to isolate 835 microsatellite loci in <jats:italic>D. longipetala</jats:italic>. Of 64 loci selected for primer design, 15 were polymorphic among 23 individuals of <jats:italic>D. longipetala</jats:italic> and 76 individuals of the heterologous subspecies <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>meziana</jats:italic> and <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>carmineo‐viridiflora</jats:italic>. Twelve and 13 of these loci were also polymorphic in one population each of <jats:italic>D. brevispicata</jats:italic> and <jats:italic>D. seramisiana</jats:italic>, respectively. Numbers of alleles per locus varied from two to 14 in <jats:italic>D. longipetala</jats:italic>, two to 12 in <jats:italic>D. meziana</jats:italic>, one to nine in <jats:italic>D. brevispicata</jats:italic>, and one to 10 in <jats:italic>D. seramisiana</jats:italic>. <jats:styled-content style="fixed-case">STRUCTURE</jats:styled-content> analyses clearly separated the taxa from each other.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 15 new microsatellite markers are promising tools for studying population genetics in <jats:italic>Deuterocohnia</jats:italic> species.</jats:p></jats:sec> |
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| imprint | Wiley, 2018 |
| imprint_str_mv | Wiley, 2018 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| series | Applications in Plant Sciences |
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| spelling | Zenk, Fides Lea Firmer, Cynthia Wöhrmann, Tina Silva, Luciana Vicente da Weising, Kurt Huettel, Bruno Paggi, Gecele Matos 2168-0450 2168-0450 Wiley Plant Science Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1002/aps3.1147 <jats:sec><jats:title>Premise of the Study</jats:title><jats:p>Microsatellite markers were developed in <jats:italic>Deuterocohnia longipetala</jats:italic> (Bromeliaceae) to investigate species and subspecies boundaries within the genus and the genetic diversity of natural populations.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>We used 454 pyrosequencing to isolate 835 microsatellite loci in <jats:italic>D. longipetala</jats:italic>. Of 64 loci selected for primer design, 15 were polymorphic among 23 individuals of <jats:italic>D. longipetala</jats:italic> and 76 individuals of the heterologous subspecies <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>meziana</jats:italic> and <jats:italic>D. meziana</jats:italic> subsp. <jats:italic>carmineo‐viridiflora</jats:italic>. Twelve and 13 of these loci were also polymorphic in one population each of <jats:italic>D. brevispicata</jats:italic> and <jats:italic>D. seramisiana</jats:italic>, respectively. Numbers of alleles per locus varied from two to 14 in <jats:italic>D. longipetala</jats:italic>, two to 12 in <jats:italic>D. meziana</jats:italic>, one to nine in <jats:italic>D. brevispicata</jats:italic>, and one to 10 in <jats:italic>D. seramisiana</jats:italic>. <jats:styled-content style="fixed-case">STRUCTURE</jats:styled-content> analyses clearly separated the taxa from each other.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 15 new microsatellite markers are promising tools for studying population genetics in <jats:italic>Deuterocohnia</jats:italic> species.</jats:p></jats:sec> Development of 15 nuclear microsatellite markers in <i>Deuterocohnia</i> (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing Applications in Plant Sciences |
| spellingShingle | Zenk, Fides Lea, Firmer, Cynthia, Wöhrmann, Tina, Silva, Luciana Vicente da, Weising, Kurt, Huettel, Bruno, Paggi, Gecele Matos, Applications in Plant Sciences, Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing, Plant Science, Ecology, Evolution, Behavior and Systematics |
| title | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_full | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_fullStr | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_full_unstemmed | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_short | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| title_sort | development of 15 nuclear microsatellite markers in <i>deuterocohnia</i> (pitcairnioideae; bromeliaceae) using 454 pyrosequencing |
| title_unstemmed | Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing |
| topic | Plant Science, Ecology, Evolution, Behavior and Systematics |
| url | http://dx.doi.org/10.1002/aps3.1147 |