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Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments
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| Zeitschriftentitel: | Angewandte Chemie International Edition |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Angewandte Chemie International Edition, 54, 2015, 40, S. 11706-11710 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. |
|---|---|
| author |
Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. |
| spellingShingle |
Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. Angewandte Chemie International Edition Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments General Chemistry Catalysis |
| author_sort |
li, zeyang |
| spelling |
Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. 1433-7851 1521-3773 Wiley General Chemistry Catalysis http://dx.doi.org/10.1002/anie.201505277 <jats:title>Abstract</jats:title><jats:p>The site‐specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self‐quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site‐specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide‐modified fluorescent single‐domain antibody fragment or an intact immunoglobulin produced in a sortase‐catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single‐domain and full‐sized antibodies without deleterious effects on antigen binding.</jats:p> Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments Angewandte Chemie International Edition |
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10.1002/anie.201505277 |
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Wiley, 2015 |
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| title |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_unstemmed |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_full |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_fullStr |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_full_unstemmed |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_short |
Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_sort |
fluorophore‐conjugated holliday junctions for generating super‐bright antibodies and antibody fragments |
| topic |
General Chemistry Catalysis |
| url |
http://dx.doi.org/10.1002/anie.201505277 |
| publishDate |
2015 |
| physical |
11706-11710 |
| description |
<jats:title>Abstract</jats:title><jats:p>The site‐specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self‐quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site‐specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide‐modified fluorescent single‐domain antibody fragment or an intact immunoglobulin produced in a sortase‐catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single‐domain and full‐sized antibodies without deleterious effects on antigen binding.</jats:p> |
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| author | Li, Zeyang, Theile, Christopher S., Chen, Guan‐Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L. |
| author_facet | Li, Zeyang, Theile, Christopher S., Chen, Guan‐Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L., Li, Zeyang, Theile, Christopher S., Chen, Guan‐Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L. |
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| container_title | Angewandte Chemie International Edition |
| container_volume | 54 |
| description | <jats:title>Abstract</jats:title><jats:p>The site‐specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self‐quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site‐specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide‐modified fluorescent single‐domain antibody fragment or an intact immunoglobulin produced in a sortase‐catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single‐domain and full‐sized antibodies without deleterious effects on antigen binding.</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9hbmllLjIwMTUwNTI3Nw |
| imprint | Wiley, 2015 |
| imprint_str_mv | Wiley, 2015 |
| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
| issn | 1433-7851, 1521-3773 |
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| physical | 11706-11710 |
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| publisher | Wiley |
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| series | Angewandte Chemie International Edition |
| source_id | 49 |
| spelling | Li, Zeyang Theile, Christopher S. Chen, Guan‐Yu Bilate, Angelina M. Duarte, Joao N. Avalos, Ana M. Fang, Tao Barberena, Roberto Sato, Shuji Ploegh, Hidde L. 1433-7851 1521-3773 Wiley General Chemistry Catalysis http://dx.doi.org/10.1002/anie.201505277 <jats:title>Abstract</jats:title><jats:p>The site‐specific modification of proteins with fluorophores can render a protein fluorescent without compromising its function. To avoid self‐quenching from multiple fluorophores installed in close proximity, we used Holliday junctions to label proteins site‐specifically. Holliday junctions enable modification with multiple fluorophores at reasonably precise spacing. We designed a Holliday junction with three of its four arms modified with a fluorophore of choice and the remaining arm equipped with a dibenzocyclooctyne substituent to render it reactive with an azide‐modified fluorescent single‐domain antibody fragment or an intact immunoglobulin produced in a sortase‐catalyzed reaction. These fluorescent Holliday junctions improve fluorescence yields for both single‐domain and full‐sized antibodies without deleterious effects on antigen binding.</jats:p> Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments Angewandte Chemie International Edition |
| spellingShingle | Li, Zeyang, Theile, Christopher S., Chen, Guan‐Yu, Bilate, Angelina M., Duarte, Joao N., Avalos, Ana M., Fang, Tao, Barberena, Roberto, Sato, Shuji, Ploegh, Hidde L., Angewandte Chemie International Edition, Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments, General Chemistry, Catalysis |
| title | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_full | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_fullStr | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_full_unstemmed | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_short | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| title_sort | fluorophore‐conjugated holliday junctions for generating super‐bright antibodies and antibody fragments |
| title_unstemmed | Fluorophore‐Conjugated Holliday Junctions for Generating Super‐Bright Antibodies and Antibody Fragments |
| topic | General Chemistry, Catalysis |
| url | http://dx.doi.org/10.1002/anie.201505277 |