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Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress
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| Zeitschriftentitel: | Environmental Toxicology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Environmental Toxicology, 35, 2020, 1, S. 5-14 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Wang, Hui Ki, Jang‐Seu Wang, Hui Ki, Jang‐Seu |
|---|---|
| author |
Wang, Hui Ki, Jang‐Seu |
| spellingShingle |
Wang, Hui Ki, Jang‐Seu Environmental Toxicology Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine |
| author_sort |
wang, hui |
| spelling |
Wang, Hui Ki, Jang‐Seu 1520-4081 1522-7278 Wiley Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine http://dx.doi.org/10.1002/tox.22837 <jats:title>Abstract</jats:title><jats:p>Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel <jats:italic>CuZnSOD</jats:italic> genes (designated as <jats:italic>CeCSD1</jats:italic> and <jats:italic>CeCSD2</jats:italic>) from the toxicity‐testing freshwater algae <jats:italic>Closterium ehrenbergii</jats:italic> and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N‐terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that <jats:italic>C. ehrenbergii</jats:italic> was well clustered with other green algae and plants. Real‐time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that <jats:italic>CeCSDs</jats:italic> are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments.</jats:p> Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga <i>Closterium ehrenbergii</i> under metal stress Environmental Toxicology |
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| title |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_unstemmed |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_full |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_fullStr |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_full_unstemmed |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_short |
Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_sort |
molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga <i>closterium ehrenbergii</i> under metal stress |
| topic |
Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine |
| url |
http://dx.doi.org/10.1002/tox.22837 |
| publishDate |
2020 |
| physical |
5-14 |
| description |
<jats:title>Abstract</jats:title><jats:p>Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel <jats:italic>CuZnSOD</jats:italic> genes (designated as <jats:italic>CeCSD1</jats:italic> and <jats:italic>CeCSD2</jats:italic>) from the toxicity‐testing freshwater algae <jats:italic>Closterium ehrenbergii</jats:italic> and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N‐terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that <jats:italic>C. ehrenbergii</jats:italic> was well clustered with other green algae and plants. Real‐time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that <jats:italic>CeCSDs</jats:italic> are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments.</jats:p> |
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| author | Wang, Hui, Ki, Jang‐Seu |
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| description | <jats:title>Abstract</jats:title><jats:p>Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel <jats:italic>CuZnSOD</jats:italic> genes (designated as <jats:italic>CeCSD1</jats:italic> and <jats:italic>CeCSD2</jats:italic>) from the toxicity‐testing freshwater algae <jats:italic>Closterium ehrenbergii</jats:italic> and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N‐terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that <jats:italic>C. ehrenbergii</jats:italic> was well clustered with other green algae and plants. Real‐time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that <jats:italic>CeCSDs</jats:italic> are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments.</jats:p> |
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| spelling | Wang, Hui Ki, Jang‐Seu 1520-4081 1522-7278 Wiley Health, Toxicology and Mutagenesis Management, Monitoring, Policy and Law Toxicology General Medicine http://dx.doi.org/10.1002/tox.22837 <jats:title>Abstract</jats:title><jats:p>Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel <jats:italic>CuZnSOD</jats:italic> genes (designated as <jats:italic>CeCSD1</jats:italic> and <jats:italic>CeCSD2</jats:italic>) from the toxicity‐testing freshwater algae <jats:italic>Closterium ehrenbergii</jats:italic> and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N‐terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that <jats:italic>C. ehrenbergii</jats:italic> was well clustered with other green algae and plants. Real‐time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that <jats:italic>CeCSDs</jats:italic> are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments.</jats:p> Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga <i>Closterium ehrenbergii</i> under metal stress Environmental Toxicology |
| spellingShingle | Wang, Hui, Ki, Jang‐Seu, Environmental Toxicology, Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress, Health, Toxicology and Mutagenesis, Management, Monitoring, Policy and Law, Toxicology, General Medicine |
| title | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_full | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_fullStr | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_full_unstemmed | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_short | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| title_sort | molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga <i>closterium ehrenbergii</i> under metal stress |
| title_unstemmed | Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress |
| topic | Health, Toxicology and Mutagenesis, Management, Monitoring, Policy and Law, Toxicology, General Medicine |
| url | http://dx.doi.org/10.1002/tox.22837 |