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Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central d...
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| Zeitschriftentitel: | Microbiology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Microbiology, 151, 2005, 3, S. 673-681 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Microbiology Society
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| Schlagwörter: |
| author_facet |
Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. |
|---|---|
| author |
Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. |
| spellingShingle |
Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. Microbiology Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain Microbiology |
| author_sort |
oh, soon-hwan |
| spelling |
Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. 1350-0872 1465-2080 Microbiology Society Microbiology http://dx.doi.org/10.1099/mic.0.27680-0 <jats:p><jats:italic>Candida albicans</jats:italic>strain SC5314 contains two<jats:italic>ALS3</jats:italic>alleles, which differ in sequence with respect to the number of copies of the 108 bp tandem repeat sequence within the central domain of the coding region. One allele (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)) has 12 tandem repeat copies while the other (<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) has 9 copies. Wild-type<jats:italic>C. albicans</jats:italic>(<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) and those containing various<jats:italic>ALS3</jats:italic>alleles (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>),<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>) and<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>)) were assayed for adhesion to monolayers of cultured vascular endothelial and pharyngeal epithelial cells. These assays showed obvious adhesive function for the larger Als3p protein, compared to a minor contribution to adhesion from the smaller protein. These functional differences in strain SC5314 prompted examination of<jats:italic>ALS3</jats:italic>allelic diversity across the five major genetic clades of<jats:italic>C. albicans</jats:italic>. This analysis focused on the number of copies of the tandem repeat sequence within the central domain of the coding region and showed a range of alleles encoding from 6 to 19 tandem repeat copies. Clades differed with respect to prevalent<jats:italic>ALS3</jats:italic>alleles and allele distribution, but were similar for the mean number of tandem repeat copies per<jats:italic>ALS3</jats:italic>allele. Analysis of allelic pairing showed clade differences and the tendency for<jats:italic>C. albicans</jats:italic>strains to encode one longer and one shorter<jats:italic>ALS3</jats:italic>allele. The allelic variability observed for<jats:italic>ALS3</jats:italic>and its functional consequences observed in strain SC5314 highlight the importance of understanding ALS allelic diversity in order to draw accurate conclusions about Als protein function.</jats:p> Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain Microbiology |
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10.1099/mic.0.27680-0 |
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Online Free |
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Biologie |
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Microbiology Society, 2005 |
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Microbiology Society, 2005 |
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1350-0872 1465-2080 |
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2005 |
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Microbiology Society |
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Microbiology |
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49 |
| title |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_unstemmed |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_full |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_fullStr |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_full_unstemmed |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_short |
Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_sort |
functional specificity of candida albicans als3p proteins and clade specificity of als3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| topic |
Microbiology |
| url |
http://dx.doi.org/10.1099/mic.0.27680-0 |
| publishDate |
2005 |
| physical |
673-681 |
| description |
<jats:p><jats:italic>Candida albicans</jats:italic>strain SC5314 contains two<jats:italic>ALS3</jats:italic>alleles, which differ in sequence with respect to the number of copies of the 108 bp tandem repeat sequence within the central domain of the coding region. One allele (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)) has 12 tandem repeat copies while the other (<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) has 9 copies. Wild-type<jats:italic>C. albicans</jats:italic>(<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) and those containing various<jats:italic>ALS3</jats:italic>alleles (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>),<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>) and<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>)) were assayed for adhesion to monolayers of cultured vascular endothelial and pharyngeal epithelial cells. These assays showed obvious adhesive function for the larger Als3p protein, compared to a minor contribution to adhesion from the smaller protein. These functional differences in strain SC5314 prompted examination of<jats:italic>ALS3</jats:italic>allelic diversity across the five major genetic clades of<jats:italic>C. albicans</jats:italic>. This analysis focused on the number of copies of the tandem repeat sequence within the central domain of the coding region and showed a range of alleles encoding from 6 to 19 tandem repeat copies. Clades differed with respect to prevalent<jats:italic>ALS3</jats:italic>alleles and allele distribution, but were similar for the mean number of tandem repeat copies per<jats:italic>ALS3</jats:italic>allele. Analysis of allelic pairing showed clade differences and the tendency for<jats:italic>C. albicans</jats:italic>strains to encode one longer and one shorter<jats:italic>ALS3</jats:italic>allele. The allelic variability observed for<jats:italic>ALS3</jats:italic>and its functional consequences observed in strain SC5314 highlight the importance of understanding ALS allelic diversity in order to draw accurate conclusions about Als protein function.</jats:p> |
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| author | Oh, Soon-Hwan, Cheng, Georgina, Nuessen, Jennifer A., Jajko, Robert, Yeater, Kathleen M., Zhao, Xiaomin, Pujol, Claude, Soll, David R., Hoyer, Lois L. |
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| description | <jats:p><jats:italic>Candida albicans</jats:italic>strain SC5314 contains two<jats:italic>ALS3</jats:italic>alleles, which differ in sequence with respect to the number of copies of the 108 bp tandem repeat sequence within the central domain of the coding region. One allele (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)) has 12 tandem repeat copies while the other (<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) has 9 copies. Wild-type<jats:italic>C. albicans</jats:italic>(<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) and those containing various<jats:italic>ALS3</jats:italic>alleles (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>),<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>) and<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>)) were assayed for adhesion to monolayers of cultured vascular endothelial and pharyngeal epithelial cells. These assays showed obvious adhesive function for the larger Als3p protein, compared to a minor contribution to adhesion from the smaller protein. These functional differences in strain SC5314 prompted examination of<jats:italic>ALS3</jats:italic>allelic diversity across the five major genetic clades of<jats:italic>C. albicans</jats:italic>. This analysis focused on the number of copies of the tandem repeat sequence within the central domain of the coding region and showed a range of alleles encoding from 6 to 19 tandem repeat copies. Clades differed with respect to prevalent<jats:italic>ALS3</jats:italic>alleles and allele distribution, but were similar for the mean number of tandem repeat copies per<jats:italic>ALS3</jats:italic>allele. Analysis of allelic pairing showed clade differences and the tendency for<jats:italic>C. albicans</jats:italic>strains to encode one longer and one shorter<jats:italic>ALS3</jats:italic>allele. The allelic variability observed for<jats:italic>ALS3</jats:italic>and its functional consequences observed in strain SC5314 highlight the importance of understanding ALS allelic diversity in order to draw accurate conclusions about Als protein function.</jats:p> |
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| spelling | Oh, Soon-Hwan Cheng, Georgina Nuessen, Jennifer A. Jajko, Robert Yeater, Kathleen M. Zhao, Xiaomin Pujol, Claude Soll, David R. Hoyer, Lois L. 1350-0872 1465-2080 Microbiology Society Microbiology http://dx.doi.org/10.1099/mic.0.27680-0 <jats:p><jats:italic>Candida albicans</jats:italic>strain SC5314 contains two<jats:italic>ALS3</jats:italic>alleles, which differ in sequence with respect to the number of copies of the 108 bp tandem repeat sequence within the central domain of the coding region. One allele (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)) has 12 tandem repeat copies while the other (<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) has 9 copies. Wild-type<jats:italic>C. albicans</jats:italic>(<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>)) and those containing various<jats:italic>ALS3</jats:italic>alleles (<jats:italic>ALS3</jats:italic>(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>),<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>ALS3</jats:italic>(<jats:italic>9</jats:italic>) and<jats:italic>als3</jats:italic>Δ(<jats:italic>12</jats:italic>)/<jats:italic>als3</jats:italic>Δ(<jats:italic>9</jats:italic>)) were assayed for adhesion to monolayers of cultured vascular endothelial and pharyngeal epithelial cells. These assays showed obvious adhesive function for the larger Als3p protein, compared to a minor contribution to adhesion from the smaller protein. These functional differences in strain SC5314 prompted examination of<jats:italic>ALS3</jats:italic>allelic diversity across the five major genetic clades of<jats:italic>C. albicans</jats:italic>. This analysis focused on the number of copies of the tandem repeat sequence within the central domain of the coding region and showed a range of alleles encoding from 6 to 19 tandem repeat copies. Clades differed with respect to prevalent<jats:italic>ALS3</jats:italic>alleles and allele distribution, but were similar for the mean number of tandem repeat copies per<jats:italic>ALS3</jats:italic>allele. Analysis of allelic pairing showed clade differences and the tendency for<jats:italic>C. albicans</jats:italic>strains to encode one longer and one shorter<jats:italic>ALS3</jats:italic>allele. The allelic variability observed for<jats:italic>ALS3</jats:italic>and its functional consequences observed in strain SC5314 highlight the importance of understanding ALS allelic diversity in order to draw accurate conclusions about Als protein function.</jats:p> Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain Microbiology |
| spellingShingle | Oh, Soon-Hwan, Cheng, Georgina, Nuessen, Jennifer A., Jajko, Robert, Yeater, Kathleen M., Zhao, Xiaomin, Pujol, Claude, Soll, David R., Hoyer, Lois L., Microbiology, Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain, Microbiology |
| title | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_full | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_fullStr | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_full_unstemmed | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_short | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_sort | functional specificity of candida albicans als3p proteins and clade specificity of als3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| title_unstemmed | Functional specificity of Candida albicans Als3p proteins and clade specificity of ALS3 alleles discriminated by the number of copies of the tandem repeat sequence in the central domain |
| topic | Microbiology |
| url | http://dx.doi.org/10.1099/mic.0.27680-0 |