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Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle
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| Zeitschriftentitel: | Proceedings of the Royal Society B: Biological Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Proceedings of the Royal Society B: Biological Sciences, 281, 2014, 1788, S. 20140786 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Royal Society
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| Schlagwörter: |
| author_facet |
Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan |
|---|---|
| author |
Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan |
| spellingShingle |
Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan Proceedings of the Royal Society B: Biological Sciences Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
| author_sort |
vickerton, paula |
| spelling |
Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan 0962-8452 1471-2954 The Royal Society General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1098/rspb.2014.0786 <jats:p> Muscular contraction plays a pivotal role in the mechanical environment of bone, but controlled muscular contractions are rarely used to study the response of bone to mechanical stimuli. Here, we use implantable stimulators to elicit programmed contractions of the rat tibialis anterior (TA) muscle. Miniature stimulators were implanted in Wistar rats ( <jats:italic>n</jats:italic> = 9) to induce contraction of the left TA every 30 s for 28 days. The right limb was used as a contralateral control. Hindlimbs were imaged using microCT. Image data were used for bone measurements, and to construct a finite-element (FE) model simulation of TA forces propagating through the bone. This simulation was used to target subsequent bone histology and measurement of micromechanical properties to areas of high strain. FE mapping of simulated strains revealed peak values in the anterodistal region of the tibia (640 µε ± 30.4 µε). This region showed significant increases in cross-sectional area (28.61%, <jats:italic>p</jats:italic> < 0.05) and bone volume (30.29%, <jats:italic>p</jats:italic> < 0.05) in the stimulated limb. Histology revealed a large region of new bone, containing clusters of chondrocytes, indicative of endochondral ossification. The new bone region had a lower elastic modulus (8.8 ± 2.2 GPa) when compared with established bone (20 ± 1.4 GPa). Our study provides compelling new evidence of the interplay between muscle and bone. </jats:p> Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle Proceedings of the Royal Society B: Biological Sciences |
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| title |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_unstemmed |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_full |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_fullStr |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_full_unstemmed |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_short |
Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_sort |
morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| topic |
General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
| url |
http://dx.doi.org/10.1098/rspb.2014.0786 |
| publishDate |
2014 |
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20140786 |
| description |
<jats:p>
Muscular contraction plays a pivotal role in the mechanical environment of bone, but controlled muscular contractions are rarely used to study the response of bone to mechanical stimuli. Here, we use implantable stimulators to elicit programmed contractions of the rat tibialis anterior (TA) muscle. Miniature stimulators were implanted in Wistar rats (
<jats:italic>n</jats:italic>
= 9) to induce contraction of the left TA every 30 s for 28 days. The right limb was used as a contralateral control. Hindlimbs were imaged using microCT. Image data were used for bone measurements, and to construct a finite-element (FE) model simulation of TA forces propagating through the bone. This simulation was used to target subsequent bone histology and measurement of micromechanical properties to areas of high strain. FE mapping of simulated strains revealed peak values in the anterodistal region of the tibia (640 µε ± 30.4 µε). This region showed significant increases in cross-sectional area (28.61%,
<jats:italic>p</jats:italic>
< 0.05) and bone volume (30.29%,
<jats:italic>p</jats:italic>
< 0.05) in the stimulated limb. Histology revealed a large region of new bone, containing clusters of chondrocytes, indicative of endochondral ossification. The new bone region had a lower elastic modulus (8.8 ± 2.2 GPa) when compared with established bone (20 ± 1.4 GPa). Our study provides compelling new evidence of the interplay between muscle and bone.
</jats:p> |
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| author | Vickerton, Paula, Jarvis, Jonathan C., Gallagher, James A., Akhtar, Riaz, Sutherland, Hazel, Jeffery, Nathan |
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| description | <jats:p> Muscular contraction plays a pivotal role in the mechanical environment of bone, but controlled muscular contractions are rarely used to study the response of bone to mechanical stimuli. Here, we use implantable stimulators to elicit programmed contractions of the rat tibialis anterior (TA) muscle. Miniature stimulators were implanted in Wistar rats ( <jats:italic>n</jats:italic> = 9) to induce contraction of the left TA every 30 s for 28 days. The right limb was used as a contralateral control. Hindlimbs were imaged using microCT. Image data were used for bone measurements, and to construct a finite-element (FE) model simulation of TA forces propagating through the bone. This simulation was used to target subsequent bone histology and measurement of micromechanical properties to areas of high strain. FE mapping of simulated strains revealed peak values in the anterodistal region of the tibia (640 µε ± 30.4 µε). This region showed significant increases in cross-sectional area (28.61%, <jats:italic>p</jats:italic> < 0.05) and bone volume (30.29%, <jats:italic>p</jats:italic> < 0.05) in the stimulated limb. Histology revealed a large region of new bone, containing clusters of chondrocytes, indicative of endochondral ossification. The new bone region had a lower elastic modulus (8.8 ± 2.2 GPa) when compared with established bone (20 ± 1.4 GPa). Our study provides compelling new evidence of the interplay between muscle and bone. </jats:p> |
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| spelling | Vickerton, Paula Jarvis, Jonathan C. Gallagher, James A. Akhtar, Riaz Sutherland, Hazel Jeffery, Nathan 0962-8452 1471-2954 The Royal Society General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1098/rspb.2014.0786 <jats:p> Muscular contraction plays a pivotal role in the mechanical environment of bone, but controlled muscular contractions are rarely used to study the response of bone to mechanical stimuli. Here, we use implantable stimulators to elicit programmed contractions of the rat tibialis anterior (TA) muscle. Miniature stimulators were implanted in Wistar rats ( <jats:italic>n</jats:italic> = 9) to induce contraction of the left TA every 30 s for 28 days. The right limb was used as a contralateral control. Hindlimbs were imaged using microCT. Image data were used for bone measurements, and to construct a finite-element (FE) model simulation of TA forces propagating through the bone. This simulation was used to target subsequent bone histology and measurement of micromechanical properties to areas of high strain. FE mapping of simulated strains revealed peak values in the anterodistal region of the tibia (640 µε ± 30.4 µε). This region showed significant increases in cross-sectional area (28.61%, <jats:italic>p</jats:italic> < 0.05) and bone volume (30.29%, <jats:italic>p</jats:italic> < 0.05) in the stimulated limb. Histology revealed a large region of new bone, containing clusters of chondrocytes, indicative of endochondral ossification. The new bone region had a lower elastic modulus (8.8 ± 2.2 GPa) when compared with established bone (20 ± 1.4 GPa). Our study provides compelling new evidence of the interplay between muscle and bone. </jats:p> Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle Proceedings of the Royal Society B: Biological Sciences |
| spellingShingle | Vickerton, Paula, Jarvis, Jonathan C., Gallagher, James A., Akhtar, Riaz, Sutherland, Hazel, Jeffery, Nathan, Proceedings of the Royal Society B: Biological Sciences, Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle, General Agricultural and Biological Sciences, General Environmental Science, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_full | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_fullStr | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_full_unstemmed | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_short | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_sort | morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| title_unstemmed | Morphological and histological adaptation of muscle and bone to loading induced by repetitive activation of muscle |
| topic | General Agricultural and Biological Sciences, General Environmental Science, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1098/rspb.2014.0786 |