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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification
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| Zeitschriftentitel: | Proceedings of the Royal Society B: Biological Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Proceedings of the Royal Society B: Biological Sciences, 279, 2012, 1726, S. 19-27 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Royal Society
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| Schlagwörter: |
| author_facet |
Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis |
|---|---|
| author |
Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis |
| spellingShingle |
Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis Proceedings of the Royal Society B: Biological Sciences Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
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tambutté, eric |
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Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis 0962-8452 1471-2954 The Royal Society General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1098/rspb.2011.0733 <jats:p>The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 Å and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 ± 21 Ohm cm<jats:sup>2</jats:sup>. We discuss the implication of our results for the transport of calcium involved in the calcification process.</jats:p> Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification Proceedings of the Royal Society B: Biological Sciences |
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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_short |
Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
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General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
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http://dx.doi.org/10.1098/rspb.2011.0733 |
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<jats:p>The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 Å and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 ± 21 Ohm cm<jats:sup>2</jats:sup>. We discuss the implication of our results for the transport of calcium involved in the calcification process.</jats:p> |
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| author | Tambutté, Eric, Tambutté, Sylvie, Segonds, Natacha, Zoccola, Didier, Venn, Alexander, Erez, Jonathan, Allemand, Denis |
| author_facet | Tambutté, Eric, Tambutté, Sylvie, Segonds, Natacha, Zoccola, Didier, Venn, Alexander, Erez, Jonathan, Allemand, Denis, Tambutté, Eric, Tambutté, Sylvie, Segonds, Natacha, Zoccola, Didier, Venn, Alexander, Erez, Jonathan, Allemand, Denis |
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| description | <jats:p>The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 Å and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 ± 21 Ohm cm<jats:sup>2</jats:sup>. We discuss the implication of our results for the transport of calcium involved in the calcification process.</jats:p> |
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| spelling | Tambutté, Eric Tambutté, Sylvie Segonds, Natacha Zoccola, Didier Venn, Alexander Erez, Jonathan Allemand, Denis 0962-8452 1471-2954 The Royal Society General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine http://dx.doi.org/10.1098/rspb.2011.0733 <jats:p>The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 Å and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 ± 21 Ohm cm<jats:sup>2</jats:sup>. We discuss the implication of our results for the transport of calcium involved in the calcification process.</jats:p> Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification Proceedings of the Royal Society B: Biological Sciences |
| spellingShingle | Tambutté, Eric, Tambutté, Sylvie, Segonds, Natacha, Zoccola, Didier, Venn, Alexander, Erez, Jonathan, Allemand, Denis, Proceedings of the Royal Society B: Biological Sciences, Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification, General Agricultural and Biological Sciences, General Environmental Science, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| title | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_full | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_fullStr | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_full_unstemmed | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_short | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_sort | calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| title_unstemmed | Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification |
| topic | General Agricultural and Biological Sciences, General Environmental Science, General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Medicine |
| url | http://dx.doi.org/10.1098/rspb.2011.0733 |