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A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test S...

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Zeitschriftentitel: Sexually Transmitted Diseases
Personen und Körperschaften: Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R.
In: Sexually Transmitted Diseases, 45, 2018, 2, S. 92-95
Format: E-Article
Sprache: Englisch
veröffentlicht:
Ovid Technologies (Wolters Kluwer Health)
Schlagwörter:
author_facet Peterson, Shelley W.
Martin, Irene
Demczuk, Walter
Hoang, Linda
Wylie, John
Lefebvre, Brigitte
Labbé, Annie-Claude
Naidu, Prenilla
Haldane, David
Mulvey, Michael R.
Peterson, Shelley W.
Martin, Irene
Demczuk, Walter
Hoang, Linda
Wylie, John
Lefebvre, Brigitte
Labbé, Annie-Claude
Naidu, Prenilla
Haldane, David
Mulvey, Michael R.
author Peterson, Shelley W.
Martin, Irene
Demczuk, Walter
Hoang, Linda
Wylie, John
Lefebvre, Brigitte
Labbé, Annie-Claude
Naidu, Prenilla
Haldane, David
Mulvey, Michael R.
spellingShingle Peterson, Shelley W.
Martin, Irene
Demczuk, Walter
Hoang, Linda
Wylie, John
Lefebvre, Brigitte
Labbé, Annie-Claude
Naidu, Prenilla
Haldane, David
Mulvey, Michael R.
Sexually Transmitted Diseases
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
Infectious Diseases
Microbiology (medical)
Public Health, Environmental and Occupational Health
Dermatology
author_sort peterson, shelley w.
spelling Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. 1537-4521 0148-5717 Ovid Technologies (Wolters Kluwer Health) Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology http://dx.doi.org/10.1097/olq.0000000000000707 <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture Sexually Transmitted Diseases
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title A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_unstemmed A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_full A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_fullStr A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_full_unstemmed A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_short A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_sort a comparison of real-time polymerase chain reaction assays for the detection of antimicrobial resistance markers and sequence typing from clinical nucleic acid amplification test samples and matched neisseria gonorrhoeae culture
topic Infectious Diseases
Microbiology (medical)
Public Health, Environmental and Occupational Health
Dermatology
url http://dx.doi.org/10.1097/olq.0000000000000707
publishDate 2018
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description <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p>
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author Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R.
author_facet Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R., Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R.
author_sort peterson, shelley w.
container_issue 2
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description <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p>
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spelling Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. 1537-4521 0148-5717 Ovid Technologies (Wolters Kluwer Health) Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology http://dx.doi.org/10.1097/olq.0000000000000707 <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture Sexually Transmitted Diseases
spellingShingle Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R., Sexually Transmitted Diseases, A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture, Infectious Diseases, Microbiology (medical), Public Health, Environmental and Occupational Health, Dermatology
title A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_full A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_fullStr A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_full_unstemmed A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_short A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
title_sort a comparison of real-time polymerase chain reaction assays for the detection of antimicrobial resistance markers and sequence typing from clinical nucleic acid amplification test samples and matched neisseria gonorrhoeae culture
title_unstemmed A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture
topic Infectious Diseases, Microbiology (medical), Public Health, Environmental and Occupational Health, Dermatology
url http://dx.doi.org/10.1097/olq.0000000000000707