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A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test S...
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Zeitschriftentitel: | Sexually Transmitted Diseases |
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Personen und Körperschaften: | , , , , , , , , , |
In: | Sexually Transmitted Diseases, 45, 2018, 2, S. 92-95 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Ovid Technologies (Wolters Kluwer Health)
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Schlagwörter: |
author_facet |
Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. |
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author |
Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. |
spellingShingle |
Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. Sexually Transmitted Diseases A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology |
author_sort |
peterson, shelley w. |
spelling |
Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. 1537-4521 0148-5717 Ovid Technologies (Wolters Kluwer Health) Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology http://dx.doi.org/10.1097/olq.0000000000000707 <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture Sexually Transmitted Diseases |
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10.1097/olq.0000000000000707 |
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Ovid Technologies (Wolters Kluwer Health), 2018 |
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0148-5717 1537-4521 |
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title |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_unstemmed |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_full |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_fullStr |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_full_unstemmed |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_short |
A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_sort |
a comparison of real-time polymerase chain reaction assays for the detection of antimicrobial resistance markers and sequence typing from clinical nucleic acid amplification test samples and matched neisseria gonorrhoeae culture |
topic |
Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology |
url |
http://dx.doi.org/10.1097/olq.0000000000000707 |
publishDate |
2018 |
physical |
92-95 |
description |
<jats:title>Abstract</jats:title>
<jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> |
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author | Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R. |
author_facet | Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R., Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R. |
author_sort | peterson, shelley w. |
container_issue | 2 |
container_start_page | 92 |
container_title | Sexually Transmitted Diseases |
container_volume | 45 |
description | <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> |
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spelling | Peterson, Shelley W. Martin, Irene Demczuk, Walter Hoang, Linda Wylie, John Lefebvre, Brigitte Labbé, Annie-Claude Naidu, Prenilla Haldane, David Mulvey, Michael R. 1537-4521 0148-5717 Ovid Technologies (Wolters Kluwer Health) Infectious Diseases Microbiology (medical) Public Health, Environmental and Occupational Health Dermatology http://dx.doi.org/10.1097/olq.0000000000000707 <jats:title>Abstract</jats:title> <jats:p>Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance–associated mutations were tested on <jats:italic toggle="yes">Neisseria gonorrhoeae</jats:italic>–positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. <jats:italic toggle="yes">N. gonorrhoeae</jats:italic> multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 <jats:italic toggle="yes">N. gonorrhoeae</jats:italic>–negative specimens showed false-positive results.</jats:p> A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture Sexually Transmitted Diseases |
spellingShingle | Peterson, Shelley W., Martin, Irene, Demczuk, Walter, Hoang, Linda, Wylie, John, Lefebvre, Brigitte, Labbé, Annie-Claude, Naidu, Prenilla, Haldane, David, Mulvey, Michael R., Sexually Transmitted Diseases, A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture, Infectious Diseases, Microbiology (medical), Public Health, Environmental and Occupational Health, Dermatology |
title | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_full | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_fullStr | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_full_unstemmed | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_short | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
title_sort | a comparison of real-time polymerase chain reaction assays for the detection of antimicrobial resistance markers and sequence typing from clinical nucleic acid amplification test samples and matched neisseria gonorrhoeae culture |
title_unstemmed | A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture |
topic | Infectious Diseases, Microbiology (medical), Public Health, Environmental and Occupational Health, Dermatology |
url | http://dx.doi.org/10.1097/olq.0000000000000707 |