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Analysis of Small RNAs Associated with Plant Senescence
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| Zeitschriftentitel: | The FASEB Journal |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | The FASEB Journal, 26, 2012, S1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J |
|---|---|
| author |
Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J |
| spellingShingle |
Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J The FASEB Journal Analysis of Small RNAs Associated with Plant Senescence Genetics Molecular Biology Biochemistry Biotechnology |
| author_sort |
wright, christopher d |
| spelling |
Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J 0892-6638 1530-6860 Wiley Genetics Molecular Biology Biochemistry Biotechnology http://dx.doi.org/10.1096/fasebj.26.1_supplement.952.7 <jats:p>Since their discovery in 1993, small RNAs have been shown to have an increasingly important role in post‐transcriptional gene regulation. MicroRNAs (miRNAs) are of particular interest because they are often conserved across many different species. This allows research on model organisms to be more easily extended to help solve problems in commercial sectors. One major problem in the agricultural sector is the decline in health during plant aging, or senescence. This process is of key importance commercially, where it can cause decreases in crop yield and postharvest quality. If the onset of senescence could be delayed or halted, based on knowledge of the mechanisms involved, this could prove very beneficial for agricultural improvement. To test the hypothesis that miRNAs participate in senescence, we constructed and sequenced a series of small RNA libraries from Arabidopsis leaf tissue at varying stages of senescence. A comparison of miRNA expression changes in these libraries provided evidence for novel senescence regulation. Using northern blots as a confirmation, we have reproduced the regulation of previously published senescence‐dependent miRNAs, as well as identified several new candidates. We have also constructed Parallel Analysis of RNA Ends (PARE) libraries to search for mRNA cleavage products due to specific miRNA‐guided cleavage, as well as discover general senescence‐related RNA decay patterns. Initial analysis of PARE data has revealed regulated cleavage of a target of one of the senescence‐regulated miRNAs at the expected site during senescence. Further analysis and validation of both small RNA as well as PARE library data will help us gain a deeper understanding of the fundamental processes involved in senescence. Supported by BARD, NIH, and HHMI.</jats:p> Analysis of Small RNAs Associated with Plant Senescence The FASEB Journal |
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| title |
Analysis of Small RNAs Associated with Plant Senescence |
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Analysis of Small RNAs Associated with Plant Senescence |
| title_full |
Analysis of Small RNAs Associated with Plant Senescence |
| title_fullStr |
Analysis of Small RNAs Associated with Plant Senescence |
| title_full_unstemmed |
Analysis of Small RNAs Associated with Plant Senescence |
| title_short |
Analysis of Small RNAs Associated with Plant Senescence |
| title_sort |
analysis of small rnas associated with plant senescence |
| topic |
Genetics Molecular Biology Biochemistry Biotechnology |
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http://dx.doi.org/10.1096/fasebj.26.1_supplement.952.7 |
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2012 |
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<jats:p>Since their discovery in 1993, small RNAs have been shown to have an increasingly important role in post‐transcriptional gene regulation. MicroRNAs (miRNAs) are of particular interest because they are often conserved across many different species. This allows research on model organisms to be more easily extended to help solve problems in commercial sectors. One major problem in the agricultural sector is the decline in health during plant aging, or senescence. This process is of key importance commercially, where it can cause decreases in crop yield and postharvest quality. If the onset of senescence could be delayed or halted, based on knowledge of the mechanisms involved, this could prove very beneficial for agricultural improvement. To test the hypothesis that miRNAs participate in senescence, we constructed and sequenced a series of small RNA libraries from Arabidopsis leaf tissue at varying stages of senescence. A comparison of miRNA expression changes in these libraries provided evidence for novel senescence regulation. Using northern blots as a confirmation, we have reproduced the regulation of previously published senescence‐dependent miRNAs, as well as identified several new candidates. We have also constructed Parallel Analysis of RNA Ends (PARE) libraries to search for mRNA cleavage products due to specific miRNA‐guided cleavage, as well as discover general senescence‐related RNA decay patterns. Initial analysis of PARE data has revealed regulated cleavage of a target of one of the senescence‐regulated miRNAs at the expected site during senescence. Further analysis and validation of both small RNA as well as PARE library data will help us gain a deeper understanding of the fundamental processes involved in senescence. Supported by BARD, NIH, and HHMI.</jats:p> |
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| author | Wright, Christopher D, Thatcher, Shawn R, Burd, Shaul, Lers, Amnon, Green, Pamela J |
| author_facet | Wright, Christopher D, Thatcher, Shawn R, Burd, Shaul, Lers, Amnon, Green, Pamela J, Wright, Christopher D, Thatcher, Shawn R, Burd, Shaul, Lers, Amnon, Green, Pamela J |
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| description | <jats:p>Since their discovery in 1993, small RNAs have been shown to have an increasingly important role in post‐transcriptional gene regulation. MicroRNAs (miRNAs) are of particular interest because they are often conserved across many different species. This allows research on model organisms to be more easily extended to help solve problems in commercial sectors. One major problem in the agricultural sector is the decline in health during plant aging, or senescence. This process is of key importance commercially, where it can cause decreases in crop yield and postharvest quality. If the onset of senescence could be delayed or halted, based on knowledge of the mechanisms involved, this could prove very beneficial for agricultural improvement. To test the hypothesis that miRNAs participate in senescence, we constructed and sequenced a series of small RNA libraries from Arabidopsis leaf tissue at varying stages of senescence. A comparison of miRNA expression changes in these libraries provided evidence for novel senescence regulation. Using northern blots as a confirmation, we have reproduced the regulation of previously published senescence‐dependent miRNAs, as well as identified several new candidates. We have also constructed Parallel Analysis of RNA Ends (PARE) libraries to search for mRNA cleavage products due to specific miRNA‐guided cleavage, as well as discover general senescence‐related RNA decay patterns. Initial analysis of PARE data has revealed regulated cleavage of a target of one of the senescence‐regulated miRNAs at the expected site during senescence. Further analysis and validation of both small RNA as well as PARE library data will help us gain a deeper understanding of the fundamental processes involved in senescence. Supported by BARD, NIH, and HHMI.</jats:p> |
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| spelling | Wright, Christopher D Thatcher, Shawn R Burd, Shaul Lers, Amnon Green, Pamela J 0892-6638 1530-6860 Wiley Genetics Molecular Biology Biochemistry Biotechnology http://dx.doi.org/10.1096/fasebj.26.1_supplement.952.7 <jats:p>Since their discovery in 1993, small RNAs have been shown to have an increasingly important role in post‐transcriptional gene regulation. MicroRNAs (miRNAs) are of particular interest because they are often conserved across many different species. This allows research on model organisms to be more easily extended to help solve problems in commercial sectors. One major problem in the agricultural sector is the decline in health during plant aging, or senescence. This process is of key importance commercially, where it can cause decreases in crop yield and postharvest quality. If the onset of senescence could be delayed or halted, based on knowledge of the mechanisms involved, this could prove very beneficial for agricultural improvement. To test the hypothesis that miRNAs participate in senescence, we constructed and sequenced a series of small RNA libraries from Arabidopsis leaf tissue at varying stages of senescence. A comparison of miRNA expression changes in these libraries provided evidence for novel senescence regulation. Using northern blots as a confirmation, we have reproduced the regulation of previously published senescence‐dependent miRNAs, as well as identified several new candidates. We have also constructed Parallel Analysis of RNA Ends (PARE) libraries to search for mRNA cleavage products due to specific miRNA‐guided cleavage, as well as discover general senescence‐related RNA decay patterns. Initial analysis of PARE data has revealed regulated cleavage of a target of one of the senescence‐regulated miRNAs at the expected site during senescence. Further analysis and validation of both small RNA as well as PARE library data will help us gain a deeper understanding of the fundamental processes involved in senescence. Supported by BARD, NIH, and HHMI.</jats:p> Analysis of Small RNAs Associated with Plant Senescence The FASEB Journal |
| spellingShingle | Wright, Christopher D, Thatcher, Shawn R, Burd, Shaul, Lers, Amnon, Green, Pamela J, The FASEB Journal, Analysis of Small RNAs Associated with Plant Senescence, Genetics, Molecular Biology, Biochemistry, Biotechnology |
| title | Analysis of Small RNAs Associated with Plant Senescence |
| title_full | Analysis of Small RNAs Associated with Plant Senescence |
| title_fullStr | Analysis of Small RNAs Associated with Plant Senescence |
| title_full_unstemmed | Analysis of Small RNAs Associated with Plant Senescence |
| title_short | Analysis of Small RNAs Associated with Plant Senescence |
| title_sort | analysis of small rnas associated with plant senescence |
| title_unstemmed | Analysis of Small RNAs Associated with Plant Senescence |
| topic | Genetics, Molecular Biology, Biochemistry, Biotechnology |
| url | http://dx.doi.org/10.1096/fasebj.26.1_supplement.952.7 |