Eintrag weiter verarbeiten
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ
Gespeichert in:
| Zeitschriftentitel: | Nucleic Acids Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , |
| In: | Nucleic Acids Research, 36, 2008, 16, S. 5166-5179 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor |
|---|---|
| author |
Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor |
| spellingShingle |
Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor Nucleic Acids Research The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ Genetics |
| author_sort |
selak, nives |
| spelling |
Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor 1362-4962 0305-1048 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkn498 <jats:title>Abstract</jats:title> <jats:p>Bloom's syndrome (BS) is a cancer predisposition disorder caused by mutation of the BLM gene, encoding a member of the RecQ helicase family. Although the phenotype of BS cells is suggestive of a role for BLM in repair of stalled or damaged replication forks, thus far there has been no direct evidence that BLM associates with any of the three human replicative DNA polymerases. Here, we show that BLM interacts specifically in vitro and in vivo with p12, the smallest subunit of human POL δ (hPOL δ). The hPOL δ enzyme, as well as the isolated p12 subunit, stimulates the DNA helicase activity of BLM. Conversely, BLM stimulates hPOL δ strand displacement activity. Our results provide the first functional link between BLM and the replicative machinery in human cells, and suggest that BLM might be recruited to sites of disrupted replication through an interaction with hPOL δ. Finally, our data also define a novel role for the poorly characterized p12 subunit of hPOL δ.</jats:p> The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ Nucleic Acids Research |
| doi_str_mv |
10.1093/nar/gkn498 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2tuNDk4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2tuNDk4 |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
Oxford University Press (OUP), 2008 |
| imprint_str_mv |
Oxford University Press (OUP), 2008 |
| issn |
1362-4962 0305-1048 |
| issn_str_mv |
1362-4962 0305-1048 |
| language |
English |
| mega_collection |
Oxford University Press (OUP) (CrossRef) |
| match_str |
selak2008thebloomssyndromehelicaseblminteractsphysicallyandfunctionallywithp12thesmallestsubunitofhumandnapolymerased |
| publishDateSort |
2008 |
| publisher |
Oxford University Press (OUP) |
| recordtype |
ai |
| record_format |
ai |
| series |
Nucleic Acids Research |
| source_id |
49 |
| title |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_unstemmed |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_full |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_fullStr |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_full_unstemmed |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_short |
The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_sort |
the bloom's syndrome helicase (blm) interacts physically and functionally with p12, the smallest subunit of human dna polymerase δ |
| topic |
Genetics |
| url |
http://dx.doi.org/10.1093/nar/gkn498 |
| publishDate |
2008 |
| physical |
5166-5179 |
| description |
<jats:title>Abstract</jats:title>
<jats:p>Bloom's syndrome (BS) is a cancer predisposition disorder caused by mutation of the BLM gene, encoding a member of the RecQ helicase family. Although the phenotype of BS cells is suggestive of a role for BLM in repair of stalled or damaged replication forks, thus far there has been no direct evidence that BLM associates with any of the three human replicative DNA polymerases. Here, we show that BLM interacts specifically in vitro and in vivo with p12, the smallest subunit of human POL δ (hPOL δ). The hPOL δ enzyme, as well as the isolated p12 subunit, stimulates the DNA helicase activity of BLM. Conversely, BLM stimulates hPOL δ strand displacement activity. Our results provide the first functional link between BLM and the replicative machinery in human cells, and suggest that BLM might be recruited to sites of disrupted replication through an interaction with hPOL δ. Finally, our data also define a novel role for the poorly characterized p12 subunit of hPOL δ.</jats:p> |
| container_issue |
16 |
| container_start_page |
5166 |
| container_title |
Nucleic Acids Research |
| container_volume |
36 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792333565371875329 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:14:21.704Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=The+Bloom%27s+syndrome+helicase+%28BLM%29+interacts+physically+and+functionally+with+p12%2C+the+smallest+subunit+of+human+DNA+polymerase+%CE%B4&rft.date=2008-09-01&genre=article&issn=0305-1048&volume=36&issue=16&spage=5166&epage=5179&pages=5166-5179&jtitle=Nucleic+Acids+Research&atitle=The+Bloom%27s+syndrome+helicase+%28BLM%29+interacts+physically+and+functionally+with+p12%2C+the+smallest+subunit+of+human+DNA+polymerase+%CE%B4&aulast=Stagljar&aufirst=Igor&rft_id=info%3Adoi%2F10.1093%2Fnar%2Fgkn498&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792333565371875329 |
| author | Selak, Nives, Bachrati, Csanád Z., Shevelev, Igor, Dietschy, Tobias, Loon, Barbara van, Jacob, Anette, Hübscher, Ulrich, Hoheisel, Joerg D., Hickson, Ian D., Stagljar, Igor |
| author_facet | Selak, Nives, Bachrati, Csanád Z., Shevelev, Igor, Dietschy, Tobias, Loon, Barbara van, Jacob, Anette, Hübscher, Ulrich, Hoheisel, Joerg D., Hickson, Ian D., Stagljar, Igor, Selak, Nives, Bachrati, Csanád Z., Shevelev, Igor, Dietschy, Tobias, Loon, Barbara van, Jacob, Anette, Hübscher, Ulrich, Hoheisel, Joerg D., Hickson, Ian D., Stagljar, Igor |
| author_sort | selak, nives |
| container_issue | 16 |
| container_start_page | 5166 |
| container_title | Nucleic Acids Research |
| container_volume | 36 |
| description | <jats:title>Abstract</jats:title> <jats:p>Bloom's syndrome (BS) is a cancer predisposition disorder caused by mutation of the BLM gene, encoding a member of the RecQ helicase family. Although the phenotype of BS cells is suggestive of a role for BLM in repair of stalled or damaged replication forks, thus far there has been no direct evidence that BLM associates with any of the three human replicative DNA polymerases. Here, we show that BLM interacts specifically in vitro and in vivo with p12, the smallest subunit of human POL δ (hPOL δ). The hPOL δ enzyme, as well as the isolated p12 subunit, stimulates the DNA helicase activity of BLM. Conversely, BLM stimulates hPOL δ strand displacement activity. Our results provide the first functional link between BLM and the replicative machinery in human cells, and suggest that BLM might be recruited to sites of disrupted replication through an interaction with hPOL δ. Finally, our data also define a novel role for the poorly characterized p12 subunit of hPOL δ.</jats:p> |
| doi_str_mv | 10.1093/nar/gkn498 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2tuNDk4 |
| imprint | Oxford University Press (OUP), 2008 |
| imprint_str_mv | Oxford University Press (OUP), 2008 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 1362-4962, 0305-1048 |
| issn_str_mv | 1362-4962, 0305-1048 |
| language | English |
| last_indexed | 2024-03-01T14:14:21.704Z |
| match_str | selak2008thebloomssyndromehelicaseblminteractsphysicallyandfunctionallywithp12thesmallestsubunitofhumandnapolymerased |
| mega_collection | Oxford University Press (OUP) (CrossRef) |
| physical | 5166-5179 |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher | Oxford University Press (OUP) |
| record_format | ai |
| recordtype | ai |
| series | Nucleic Acids Research |
| source_id | 49 |
| spelling | Selak, Nives Bachrati, Csanád Z. Shevelev, Igor Dietschy, Tobias Loon, Barbara van Jacob, Anette Hübscher, Ulrich Hoheisel, Joerg D. Hickson, Ian D. Stagljar, Igor 1362-4962 0305-1048 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkn498 <jats:title>Abstract</jats:title> <jats:p>Bloom's syndrome (BS) is a cancer predisposition disorder caused by mutation of the BLM gene, encoding a member of the RecQ helicase family. Although the phenotype of BS cells is suggestive of a role for BLM in repair of stalled or damaged replication forks, thus far there has been no direct evidence that BLM associates with any of the three human replicative DNA polymerases. Here, we show that BLM interacts specifically in vitro and in vivo with p12, the smallest subunit of human POL δ (hPOL δ). The hPOL δ enzyme, as well as the isolated p12 subunit, stimulates the DNA helicase activity of BLM. Conversely, BLM stimulates hPOL δ strand displacement activity. Our results provide the first functional link between BLM and the replicative machinery in human cells, and suggest that BLM might be recruited to sites of disrupted replication through an interaction with hPOL δ. Finally, our data also define a novel role for the poorly characterized p12 subunit of hPOL δ.</jats:p> The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ Nucleic Acids Research |
| spellingShingle | Selak, Nives, Bachrati, Csanád Z., Shevelev, Igor, Dietschy, Tobias, Loon, Barbara van, Jacob, Anette, Hübscher, Ulrich, Hoheisel, Joerg D., Hickson, Ian D., Stagljar, Igor, Nucleic Acids Research, The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ, Genetics |
| title | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_full | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_fullStr | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_full_unstemmed | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_short | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| title_sort | the bloom's syndrome helicase (blm) interacts physically and functionally with p12, the smallest subunit of human dna polymerase δ |
| title_unstemmed | The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase δ |
| topic | Genetics |
| url | http://dx.doi.org/10.1093/nar/gkn498 |