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A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
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| Zeitschriftentitel: | Nucleic Acids Research |
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| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Nucleic Acids Research, 47, 2019, 16, S. 8807-8820 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
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| Schlagwörter: |
| author_facet |
Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili |
|---|---|
| author |
Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili |
| spellingShingle |
Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili Nucleic Acids Research A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling Genetics |
| author_sort |
kasari, villu |
| spelling |
Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili 0305-1048 1362-4962 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkz600 <jats:title>Abstract</jats:title><jats:p>Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3′-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.</jats:p> A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling Nucleic Acids Research |
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| title |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_unstemmed |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_full |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_fullStr |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_full_unstemmed |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_short |
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_sort |
a role for the saccharomyces cerevisiae abcf protein new1 in translation termination/recycling |
| topic |
Genetics |
| url |
http://dx.doi.org/10.1093/nar/gkz600 |
| publishDate |
2019 |
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8807-8820 |
| description |
<jats:title>Abstract</jats:title><jats:p>Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3′-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.</jats:p> |
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| author | Kasari, Villu, Pochopien, Agnieszka A, Margus, Tõnu, Murina, Victoriia, Turnbull, Kathryn, Zhou, Yang, Nissan, Tracy, Graf, Michael, Nováček, Jiří, Atkinson, Gemma C, Johansson, Marcus J O, Wilson, Daniel N, Hauryliuk, Vasili |
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| description | <jats:title>Abstract</jats:title><jats:p>Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3′-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.</jats:p> |
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| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| spelling | Kasari, Villu Pochopien, Agnieszka A Margus, Tõnu Murina, Victoriia Turnbull, Kathryn Zhou, Yang Nissan, Tracy Graf, Michael Nováček, Jiří Atkinson, Gemma C Johansson, Marcus J O Wilson, Daniel N Hauryliuk, Vasili 0305-1048 1362-4962 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkz600 <jats:title>Abstract</jats:title><jats:p>Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3′-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.</jats:p> A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling Nucleic Acids Research |
| spellingShingle | Kasari, Villu, Pochopien, Agnieszka A, Margus, Tõnu, Murina, Victoriia, Turnbull, Kathryn, Zhou, Yang, Nissan, Tracy, Graf, Michael, Nováček, Jiří, Atkinson, Gemma C, Johansson, Marcus J O, Wilson, Daniel N, Hauryliuk, Vasili, Nucleic Acids Research, A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling, Genetics |
| title | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_full | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_fullStr | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_full_unstemmed | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_short | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| title_sort | a role for the saccharomyces cerevisiae abcf protein new1 in translation termination/recycling |
| title_unstemmed | A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling |
| topic | Genetics |
| url | http://dx.doi.org/10.1093/nar/gkz600 |