Eintrag weiter verarbeiten
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin
Gespeichert in:
| Zeitschriftentitel: | Nucleic Acids Research |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Nucleic Acids Research, 47, 2019, 17, S. 8988-9004 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Oxford University Press (OUP)
|
| Schlagwörter: |
| author_facet |
Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie |
|---|---|
| author |
Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie |
| spellingShingle |
Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie Nucleic Acids Research Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin Genetics |
| author_sort |
georges, adrien |
| spelling |
Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie 0305-1048 1362-4962 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkz598 <jats:title>Abstract</jats:title><jats:p>Transcription and maintenance of genome integrity are fundamental cellular functions. Deregulation of transcription and defects in DNA repair lead to serious pathologies. The Mediator complex links RNA polymerase (Pol) II transcription and nucleotide excision repair via Rad2/XPG endonuclease. However, the functional interplay between Rad2/XPG, Mediator and Pol II remains to be determined. In this study, we investigated their functional dynamics using genomic and genetic approaches. In a mutant affected in Pol II phosphorylation leading to Mediator stabilization on core promoters, Rad2 genome-wide occupancy shifts towards core promoters following that of Mediator, but decreases on transcribed regions together with Pol II. Specific Mediator mutations increase UV sensitivity, reduce Rad2 recruitment to transcribed regions, lead to uncoupling of Rad2, Mediator and Pol II and to colethality with deletion of Rpb9 Pol II subunit involved in transcription-coupled repair. We provide new insights into the functional interplay between Rad2, Mediator and Pol II and propose that dynamic interactions with Mediator and Pol II are involved in Rad2 loading to the chromatin. Our work contributes to the understanding of the complex link between transcription and DNA repair machineries, dysfunction of which leads to severe diseases.</jats:p> Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin Nucleic Acids Research |
| doi_str_mv |
10.1093/nar/gkz598 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2t6NTk4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2t6NTk4 |
| institution |
DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
Oxford University Press (OUP), 2019 |
| imprint_str_mv |
Oxford University Press (OUP), 2019 |
| issn |
0305-1048 1362-4962 |
| issn_str_mv |
0305-1048 1362-4962 |
| language |
English |
| mega_collection |
Oxford University Press (OUP) (CrossRef) |
| match_str |
georges2019functionalinterplaybetweenmediatorandrnapolymeraseiiinrad2xpgloadingtothechromatin |
| publishDateSort |
2019 |
| publisher |
Oxford University Press (OUP) |
| recordtype |
ai |
| record_format |
ai |
| series |
Nucleic Acids Research |
| source_id |
49 |
| title |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_unstemmed |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_full |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_fullStr |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_full_unstemmed |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_short |
Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_sort |
functional interplay between mediator and rna polymerase ii in rad2/xpg loading to the chromatin |
| topic |
Genetics |
| url |
http://dx.doi.org/10.1093/nar/gkz598 |
| publishDate |
2019 |
| physical |
8988-9004 |
| description |
<jats:title>Abstract</jats:title><jats:p>Transcription and maintenance of genome integrity are fundamental cellular functions. Deregulation of transcription and defects in DNA repair lead to serious pathologies. The Mediator complex links RNA polymerase (Pol) II transcription and nucleotide excision repair via Rad2/XPG endonuclease. However, the functional interplay between Rad2/XPG, Mediator and Pol II remains to be determined. In this study, we investigated their functional dynamics using genomic and genetic approaches. In a mutant affected in Pol II phosphorylation leading to Mediator stabilization on core promoters, Rad2 genome-wide occupancy shifts towards core promoters following that of Mediator, but decreases on transcribed regions together with Pol II. Specific Mediator mutations increase UV sensitivity, reduce Rad2 recruitment to transcribed regions, lead to uncoupling of Rad2, Mediator and Pol II and to colethality with deletion of Rpb9 Pol II subunit involved in transcription-coupled repair. We provide new insights into the functional interplay between Rad2, Mediator and Pol II and propose that dynamic interactions with Mediator and Pol II are involved in Rad2 loading to the chromatin. Our work contributes to the understanding of the complex link between transcription and DNA repair machineries, dysfunction of which leads to severe diseases.</jats:p> |
| container_issue |
17 |
| container_start_page |
8988 |
| container_title |
Nucleic Acids Research |
| container_volume |
47 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346587047919617 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:41:45.873Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Functional+interplay+between+Mediator+and+RNA+polymerase+II+in+Rad2%2FXPG+loading+to+the+chromatin&rft.date=2019-09-26&genre=article&issn=1362-4962&volume=47&issue=17&spage=8988&epage=9004&pages=8988-9004&jtitle=Nucleic+Acids+Research&atitle=Functional+interplay+between+Mediator+and+RNA+polymerase+II+in+Rad2%2FXPG+loading+to+the+chromatin&aulast=Soutourina&aufirst=Julie&rft_id=info%3Adoi%2F10.1093%2Fnar%2Fgkz598&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346587047919617 |
| author | Georges, Adrien, Gopaul, Diyavarshini, Denby Wilkes, Cyril, Giordanengo Aiach, Nathalie, Novikova, Elizaveta, Barrault, Marie-Bénédicte, Alibert, Olivier, Soutourina, Julie |
| author_facet | Georges, Adrien, Gopaul, Diyavarshini, Denby Wilkes, Cyril, Giordanengo Aiach, Nathalie, Novikova, Elizaveta, Barrault, Marie-Bénédicte, Alibert, Olivier, Soutourina, Julie, Georges, Adrien, Gopaul, Diyavarshini, Denby Wilkes, Cyril, Giordanengo Aiach, Nathalie, Novikova, Elizaveta, Barrault, Marie-Bénédicte, Alibert, Olivier, Soutourina, Julie |
| author_sort | georges, adrien |
| container_issue | 17 |
| container_start_page | 8988 |
| container_title | Nucleic Acids Research |
| container_volume | 47 |
| description | <jats:title>Abstract</jats:title><jats:p>Transcription and maintenance of genome integrity are fundamental cellular functions. Deregulation of transcription and defects in DNA repair lead to serious pathologies. The Mediator complex links RNA polymerase (Pol) II transcription and nucleotide excision repair via Rad2/XPG endonuclease. However, the functional interplay between Rad2/XPG, Mediator and Pol II remains to be determined. In this study, we investigated their functional dynamics using genomic and genetic approaches. In a mutant affected in Pol II phosphorylation leading to Mediator stabilization on core promoters, Rad2 genome-wide occupancy shifts towards core promoters following that of Mediator, but decreases on transcribed regions together with Pol II. Specific Mediator mutations increase UV sensitivity, reduce Rad2 recruitment to transcribed regions, lead to uncoupling of Rad2, Mediator and Pol II and to colethality with deletion of Rpb9 Pol II subunit involved in transcription-coupled repair. We provide new insights into the functional interplay between Rad2, Mediator and Pol II and propose that dynamic interactions with Mediator and Pol II are involved in Rad2 loading to the chromatin. Our work contributes to the understanding of the complex link between transcription and DNA repair machineries, dysfunction of which leads to severe diseases.</jats:p> |
| doi_str_mv | 10.1093/nar/gkz598 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9uYXIvZ2t6NTk4 |
| imprint | Oxford University Press (OUP), 2019 |
| imprint_str_mv | Oxford University Press (OUP), 2019 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 0305-1048, 1362-4962 |
| issn_str_mv | 0305-1048, 1362-4962 |
| language | English |
| last_indexed | 2024-03-01T17:41:45.873Z |
| match_str | georges2019functionalinterplaybetweenmediatorandrnapolymeraseiiinrad2xpgloadingtothechromatin |
| mega_collection | Oxford University Press (OUP) (CrossRef) |
| physical | 8988-9004 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford University Press (OUP) |
| record_format | ai |
| recordtype | ai |
| series | Nucleic Acids Research |
| source_id | 49 |
| spelling | Georges, Adrien Gopaul, Diyavarshini Denby Wilkes, Cyril Giordanengo Aiach, Nathalie Novikova, Elizaveta Barrault, Marie-Bénédicte Alibert, Olivier Soutourina, Julie 0305-1048 1362-4962 Oxford University Press (OUP) Genetics http://dx.doi.org/10.1093/nar/gkz598 <jats:title>Abstract</jats:title><jats:p>Transcription and maintenance of genome integrity are fundamental cellular functions. Deregulation of transcription and defects in DNA repair lead to serious pathologies. The Mediator complex links RNA polymerase (Pol) II transcription and nucleotide excision repair via Rad2/XPG endonuclease. However, the functional interplay between Rad2/XPG, Mediator and Pol II remains to be determined. In this study, we investigated their functional dynamics using genomic and genetic approaches. In a mutant affected in Pol II phosphorylation leading to Mediator stabilization on core promoters, Rad2 genome-wide occupancy shifts towards core promoters following that of Mediator, but decreases on transcribed regions together with Pol II. Specific Mediator mutations increase UV sensitivity, reduce Rad2 recruitment to transcribed regions, lead to uncoupling of Rad2, Mediator and Pol II and to colethality with deletion of Rpb9 Pol II subunit involved in transcription-coupled repair. We provide new insights into the functional interplay between Rad2, Mediator and Pol II and propose that dynamic interactions with Mediator and Pol II are involved in Rad2 loading to the chromatin. Our work contributes to the understanding of the complex link between transcription and DNA repair machineries, dysfunction of which leads to severe diseases.</jats:p> Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin Nucleic Acids Research |
| spellingShingle | Georges, Adrien, Gopaul, Diyavarshini, Denby Wilkes, Cyril, Giordanengo Aiach, Nathalie, Novikova, Elizaveta, Barrault, Marie-Bénédicte, Alibert, Olivier, Soutourina, Julie, Nucleic Acids Research, Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin, Genetics |
| title | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_full | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_fullStr | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_full_unstemmed | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_short | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| title_sort | functional interplay between mediator and rna polymerase ii in rad2/xpg loading to the chromatin |
| title_unstemmed | Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin |
| topic | Genetics |
| url | http://dx.doi.org/10.1093/nar/gkz598 |