Eintrag weiter verarbeiten
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether
Gespeichert in:
Zeitschriftentitel: | Toxicological Sciences |
---|---|
Personen und Körperschaften: | , , , |
In: | Toxicological Sciences, 169, 2019, 2, S. 399-408 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Oxford University Press (OUP)
|
Schlagwörter: |
author_facet |
Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim |
---|---|
author |
Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim |
spellingShingle |
Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim Toxicological Sciences Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether Toxicology |
author_sort |
stermer, angela r |
spelling |
Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim 1096-6080 1096-0929 Oxford University Press (OUP) Toxicology http://dx.doi.org/10.1093/toxsci/kfz041 <jats:title>Abstract</jats:title> <jats:p>Testicular histology and semen parameters are considered the gold standards when determining male reproductive toxicity. Ethylene glycol monomethyl ether (EGME) is a testicular toxicant with well-described effects on histopathology and sperm parameters. To compare the predictivity and sensitivity of molecular biomarkers of testicular toxicity to the traditional endpoints, small RNAs in the sperm were analyzed by next generation RNA-sequencing (RNA-seq). Adult rats were exposed to 0, 50, 60, or 75 mg/kg EGME by oral gavage for 5 consecutive days. Testis histology, epididymal sperm motility, and sperm small RNAs, including microRNAs (miRNAs), mRNA fragments, piwi-interacting RNAs (piRNAs), and tRNA fragments (tRFs), were analyzed 5 weeks after cessation of exposure. Testicular histology showed a significant dose-dependent increase in retained spermatid heads (RSH), while sperm motility declined with increasing dose. RNA-sequencing of sperm small RNAs was used to identify significant dose-dependent changes in percent mRNA fragments (of total reads), percent miRNAs (of total reads), average tRF length, average piRNA length, and piRNA and tRF length-distributions. Discriminant analysis showed relatively low predictivity of exposure based on RSH or motility compared to the average read length of all assigned RNAs. Benchmark dose (BMD) modeling resulted in a BMD of 62 mg/kg using RSH, whereas average read length of all assigned RNAs resulted in a BMD of 47 mg/kg. These results showed that sperm small RNAs are sensitive and predictive biomarkers of EGME-induced male reproductive toxicity.</jats:p> Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether Toxicological Sciences |
doi_str_mv |
10.1093/toxsci/kfz041 |
facet_avail |
Online Free |
finc_class_facet |
Chemie und Pharmazie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My90b3hzY2kva2Z6MDQx |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My90b3hzY2kva2Z6MDQx |
institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
imprint |
Oxford University Press (OUP), 2019 |
imprint_str_mv |
Oxford University Press (OUP), 2019 |
issn |
1096-6080 1096-0929 |
issn_str_mv |
1096-6080 1096-0929 |
language |
English |
mega_collection |
Oxford University Press (OUP) (CrossRef) |
match_str |
stermer2019smallrnasinratspermareapredictiveandsensitivebiomarkerofexposuretothetesticulartoxicantethyleneglycolmonomethylether |
publishDateSort |
2019 |
publisher |
Oxford University Press (OUP) |
recordtype |
ai |
record_format |
ai |
series |
Toxicological Sciences |
source_id |
49 |
title |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_unstemmed |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_full |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_fullStr |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_full_unstemmed |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_short |
Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_sort |
small rnas in rat sperm are a predictive and sensitive biomarker of exposure to the testicular toxicant ethylene glycol monomethyl ether |
topic |
Toxicology |
url |
http://dx.doi.org/10.1093/toxsci/kfz041 |
publishDate |
2019 |
physical |
399-408 |
description |
<jats:title>Abstract</jats:title>
<jats:p>Testicular histology and semen parameters are considered the gold standards when determining male reproductive toxicity. Ethylene glycol monomethyl ether (EGME) is a testicular toxicant with well-described effects on histopathology and sperm parameters. To compare the predictivity and sensitivity of molecular biomarkers of testicular toxicity to the traditional endpoints, small RNAs in the sperm were analyzed by next generation RNA-sequencing (RNA-seq). Adult rats were exposed to 0, 50, 60, or 75 mg/kg EGME by oral gavage for 5 consecutive days. Testis histology, epididymal sperm motility, and sperm small RNAs, including microRNAs (miRNAs), mRNA fragments, piwi-interacting RNAs (piRNAs), and tRNA fragments (tRFs), were analyzed 5 weeks after cessation of exposure. Testicular histology showed a significant dose-dependent increase in retained spermatid heads (RSH), while sperm motility declined with increasing dose. RNA-sequencing of sperm small RNAs was used to identify significant dose-dependent changes in percent mRNA fragments (of total reads), percent miRNAs (of total reads), average tRF length, average piRNA length, and piRNA and tRF length-distributions. Discriminant analysis showed relatively low predictivity of exposure based on RSH or motility compared to the average read length of all assigned RNAs. Benchmark dose (BMD) modeling resulted in a BMD of 62 mg/kg using RSH, whereas average read length of all assigned RNAs resulted in a BMD of 47 mg/kg. These results showed that sperm small RNAs are sensitive and predictive biomarkers of EGME-induced male reproductive toxicity.</jats:p> |
container_issue |
2 |
container_start_page |
399 |
container_title |
Toxicological Sciences |
container_volume |
169 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792334887777206276 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T14:35:27.146Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Small+RNAs+in+Rat+Sperm+Are+a+Predictive+and+Sensitive+Biomarker+of+Exposure+to+the+Testicular+Toxicant+Ethylene+Glycol+Monomethyl+Ether&rft.date=2019-06-01&genre=article&issn=1096-0929&volume=169&issue=2&spage=399&epage=408&pages=399-408&jtitle=Toxicological+Sciences&atitle=Small+RNAs+in+Rat+Sperm+Are+a+Predictive+and+Sensitive+Biomarker+of+Exposure+to+the+Testicular+Toxicant+Ethylene+Glycol+Monomethyl+Ether&aulast=Boekelheide&aufirst=Kim&rft_id=info%3Adoi%2F10.1093%2Ftoxsci%2Fkfz041&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792334887777206276 |
author | Stermer, Angela R, Reyes, Gerardo, Hall, Susan J, Boekelheide, Kim |
author_facet | Stermer, Angela R, Reyes, Gerardo, Hall, Susan J, Boekelheide, Kim, Stermer, Angela R, Reyes, Gerardo, Hall, Susan J, Boekelheide, Kim |
author_sort | stermer, angela r |
container_issue | 2 |
container_start_page | 399 |
container_title | Toxicological Sciences |
container_volume | 169 |
description | <jats:title>Abstract</jats:title> <jats:p>Testicular histology and semen parameters are considered the gold standards when determining male reproductive toxicity. Ethylene glycol monomethyl ether (EGME) is a testicular toxicant with well-described effects on histopathology and sperm parameters. To compare the predictivity and sensitivity of molecular biomarkers of testicular toxicity to the traditional endpoints, small RNAs in the sperm were analyzed by next generation RNA-sequencing (RNA-seq). Adult rats were exposed to 0, 50, 60, or 75 mg/kg EGME by oral gavage for 5 consecutive days. Testis histology, epididymal sperm motility, and sperm small RNAs, including microRNAs (miRNAs), mRNA fragments, piwi-interacting RNAs (piRNAs), and tRNA fragments (tRFs), were analyzed 5 weeks after cessation of exposure. Testicular histology showed a significant dose-dependent increase in retained spermatid heads (RSH), while sperm motility declined with increasing dose. RNA-sequencing of sperm small RNAs was used to identify significant dose-dependent changes in percent mRNA fragments (of total reads), percent miRNAs (of total reads), average tRF length, average piRNA length, and piRNA and tRF length-distributions. Discriminant analysis showed relatively low predictivity of exposure based on RSH or motility compared to the average read length of all assigned RNAs. Benchmark dose (BMD) modeling resulted in a BMD of 62 mg/kg using RSH, whereas average read length of all assigned RNAs resulted in a BMD of 47 mg/kg. These results showed that sperm small RNAs are sensitive and predictive biomarkers of EGME-induced male reproductive toxicity.</jats:p> |
doi_str_mv | 10.1093/toxsci/kfz041 |
facet_avail | Online, Free |
finc_class_facet | Chemie und Pharmazie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My90b3hzY2kva2Z6MDQx |
imprint | Oxford University Press (OUP), 2019 |
imprint_str_mv | Oxford University Press (OUP), 2019 |
institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
issn | 1096-6080, 1096-0929 |
issn_str_mv | 1096-6080, 1096-0929 |
language | English |
last_indexed | 2024-03-01T14:35:27.146Z |
match_str | stermer2019smallrnasinratspermareapredictiveandsensitivebiomarkerofexposuretothetesticulartoxicantethyleneglycolmonomethylether |
mega_collection | Oxford University Press (OUP) (CrossRef) |
physical | 399-408 |
publishDate | 2019 |
publishDateSort | 2019 |
publisher | Oxford University Press (OUP) |
record_format | ai |
recordtype | ai |
series | Toxicological Sciences |
source_id | 49 |
spelling | Stermer, Angela R Reyes, Gerardo Hall, Susan J Boekelheide, Kim 1096-6080 1096-0929 Oxford University Press (OUP) Toxicology http://dx.doi.org/10.1093/toxsci/kfz041 <jats:title>Abstract</jats:title> <jats:p>Testicular histology and semen parameters are considered the gold standards when determining male reproductive toxicity. Ethylene glycol monomethyl ether (EGME) is a testicular toxicant with well-described effects on histopathology and sperm parameters. To compare the predictivity and sensitivity of molecular biomarkers of testicular toxicity to the traditional endpoints, small RNAs in the sperm were analyzed by next generation RNA-sequencing (RNA-seq). Adult rats were exposed to 0, 50, 60, or 75 mg/kg EGME by oral gavage for 5 consecutive days. Testis histology, epididymal sperm motility, and sperm small RNAs, including microRNAs (miRNAs), mRNA fragments, piwi-interacting RNAs (piRNAs), and tRNA fragments (tRFs), were analyzed 5 weeks after cessation of exposure. Testicular histology showed a significant dose-dependent increase in retained spermatid heads (RSH), while sperm motility declined with increasing dose. RNA-sequencing of sperm small RNAs was used to identify significant dose-dependent changes in percent mRNA fragments (of total reads), percent miRNAs (of total reads), average tRF length, average piRNA length, and piRNA and tRF length-distributions. Discriminant analysis showed relatively low predictivity of exposure based on RSH or motility compared to the average read length of all assigned RNAs. Benchmark dose (BMD) modeling resulted in a BMD of 62 mg/kg using RSH, whereas average read length of all assigned RNAs resulted in a BMD of 47 mg/kg. These results showed that sperm small RNAs are sensitive and predictive biomarkers of EGME-induced male reproductive toxicity.</jats:p> Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether Toxicological Sciences |
spellingShingle | Stermer, Angela R, Reyes, Gerardo, Hall, Susan J, Boekelheide, Kim, Toxicological Sciences, Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether, Toxicology |
title | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_full | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_fullStr | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_full_unstemmed | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_short | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
title_sort | small rnas in rat sperm are a predictive and sensitive biomarker of exposure to the testicular toxicant ethylene glycol monomethyl ether |
title_unstemmed | Small RNAs in Rat Sperm Are a Predictive and Sensitive Biomarker of Exposure to the Testicular Toxicant Ethylene Glycol Monomethyl Ether |
topic | Toxicology |
url | http://dx.doi.org/10.1093/toxsci/kfz041 |