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Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Molecular Biology of the Cell, 21, 2010, 19, S. 3396-3408 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
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| Schlagwörter: |
| author_facet |
Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. |
|---|---|
| author |
Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. |
| spellingShingle |
Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. Molecular Biology of the Cell Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly Cell Biology Molecular Biology |
| author_sort |
davies, brian a. |
| spelling |
Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e10-06-0512 <jats:p> ESCRT-III undergoes dynamic assembly and disassembly to facilitate membrane exvagination processes including multivesicular body (MVB) formation, enveloped virus budding, and membrane abscission during cytokinesis. The AAA-ATPase Vps4 is required for ESCRT-III disassembly, however the coordination of Vps4 ATP hydrolysis with ESCRT-III binding and disassembly is not understood. Vps4 ATP hydrolysis has been proposed to execute ESCRT-III disassembly as either a stable oligomer or an unstable oligomer whose dissociation drives ESCRT-III disassembly. An in vitro ESCRT-III disassembly assay was developed to analyze Vps4 function during this process. The studies presented here support a model in which Vps4 acts as a stable oligomer during ATP hydrolysis and ESCRT-III disassembly. Moreover, Vps4 oligomer binding to ESCRT-III induces coordination of ATP hydrolysis at the level of individual Vps4 subunits. These results suggest that Vps4 functions as a stable oligomer that acts upon individual ESCRT-III subunits to facilitate ESCRT-III disassembly. </jats:p> Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly Molecular Biology of the Cell |
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| title |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_unstemmed |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_full |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_fullStr |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_full_unstemmed |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_short |
Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_sort |
coordination of substrate binding and atp hydrolysis in vps4-mediated escrt-iii disassembly |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1091/mbc.e10-06-0512 |
| publishDate |
2010 |
| physical |
3396-3408 |
| description |
<jats:p> ESCRT-III undergoes dynamic assembly and disassembly to facilitate membrane exvagination processes including multivesicular body (MVB) formation, enveloped virus budding, and membrane abscission during cytokinesis. The AAA-ATPase Vps4 is required for ESCRT-III disassembly, however the coordination of Vps4 ATP hydrolysis with ESCRT-III binding and disassembly is not understood. Vps4 ATP hydrolysis has been proposed to execute ESCRT-III disassembly as either a stable oligomer or an unstable oligomer whose dissociation drives ESCRT-III disassembly. An in vitro ESCRT-III disassembly assay was developed to analyze Vps4 function during this process. The studies presented here support a model in which Vps4 acts as a stable oligomer during ATP hydrolysis and ESCRT-III disassembly. Moreover, Vps4 oligomer binding to ESCRT-III induces coordination of ATP hydrolysis at the level of individual Vps4 subunits. These results suggest that Vps4 functions as a stable oligomer that acts upon individual ESCRT-III subunits to facilitate ESCRT-III disassembly. </jats:p> |
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| author | Davies, Brian A., Azmi, Ishara F., Payne, Johanna, Shestakova, Anna, Horazdovsky, Bruce F., Babst, Markus, Katzmann, David J. |
| author_facet | Davies, Brian A., Azmi, Ishara F., Payne, Johanna, Shestakova, Anna, Horazdovsky, Bruce F., Babst, Markus, Katzmann, David J., Davies, Brian A., Azmi, Ishara F., Payne, Johanna, Shestakova, Anna, Horazdovsky, Bruce F., Babst, Markus, Katzmann, David J. |
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| description | <jats:p> ESCRT-III undergoes dynamic assembly and disassembly to facilitate membrane exvagination processes including multivesicular body (MVB) formation, enveloped virus budding, and membrane abscission during cytokinesis. The AAA-ATPase Vps4 is required for ESCRT-III disassembly, however the coordination of Vps4 ATP hydrolysis with ESCRT-III binding and disassembly is not understood. Vps4 ATP hydrolysis has been proposed to execute ESCRT-III disassembly as either a stable oligomer or an unstable oligomer whose dissociation drives ESCRT-III disassembly. An in vitro ESCRT-III disassembly assay was developed to analyze Vps4 function during this process. The studies presented here support a model in which Vps4 acts as a stable oligomer during ATP hydrolysis and ESCRT-III disassembly. Moreover, Vps4 oligomer binding to ESCRT-III induces coordination of ATP hydrolysis at the level of individual Vps4 subunits. These results suggest that Vps4 functions as a stable oligomer that acts upon individual ESCRT-III subunits to facilitate ESCRT-III disassembly. </jats:p> |
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| imprint_str_mv | American Society for Cell Biology (ASCB), 2010 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4 |
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| spelling | Davies, Brian A. Azmi, Ishara F. Payne, Johanna Shestakova, Anna Horazdovsky, Bruce F. Babst, Markus Katzmann, David J. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e10-06-0512 <jats:p> ESCRT-III undergoes dynamic assembly and disassembly to facilitate membrane exvagination processes including multivesicular body (MVB) formation, enveloped virus budding, and membrane abscission during cytokinesis. The AAA-ATPase Vps4 is required for ESCRT-III disassembly, however the coordination of Vps4 ATP hydrolysis with ESCRT-III binding and disassembly is not understood. Vps4 ATP hydrolysis has been proposed to execute ESCRT-III disassembly as either a stable oligomer or an unstable oligomer whose dissociation drives ESCRT-III disassembly. An in vitro ESCRT-III disassembly assay was developed to analyze Vps4 function during this process. The studies presented here support a model in which Vps4 acts as a stable oligomer during ATP hydrolysis and ESCRT-III disassembly. Moreover, Vps4 oligomer binding to ESCRT-III induces coordination of ATP hydrolysis at the level of individual Vps4 subunits. These results suggest that Vps4 functions as a stable oligomer that acts upon individual ESCRT-III subunits to facilitate ESCRT-III disassembly. </jats:p> Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly Molecular Biology of the Cell |
| spellingShingle | Davies, Brian A., Azmi, Ishara F., Payne, Johanna, Shestakova, Anna, Horazdovsky, Bruce F., Babst, Markus, Katzmann, David J., Molecular Biology of the Cell, Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly, Cell Biology, Molecular Biology |
| title | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_full | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_fullStr | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_full_unstemmed | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_short | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| title_sort | coordination of substrate binding and atp hydrolysis in vps4-mediated escrt-iii disassembly |
| title_unstemmed | Coordination of Substrate Binding and ATP Hydrolysis in Vps4-Mediated ESCRT-III Disassembly |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e10-06-0512 |