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Probing cellular mechanics with acoustic force spectroscopy
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , |
| In: | Molecular Biology of the Cell, 29, 2018, 16, S. 2005-2011 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
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| Schlagwörter: |
| author_facet |
Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. |
|---|---|
| author |
Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. |
| spellingShingle |
Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. Molecular Biology of the Cell Probing cellular mechanics with acoustic force spectroscopy Cell Biology Molecular Biology |
| author_sort |
sorkin, raya |
| spelling |
Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e18-03-0154 <jats:p>A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.</jats:p> Probing cellular mechanics with acoustic force spectroscopy Molecular Biology of the Cell |
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| title |
Probing cellular mechanics with acoustic force spectroscopy |
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Probing cellular mechanics with acoustic force spectroscopy |
| title_full |
Probing cellular mechanics with acoustic force spectroscopy |
| title_fullStr |
Probing cellular mechanics with acoustic force spectroscopy |
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Probing cellular mechanics with acoustic force spectroscopy |
| title_short |
Probing cellular mechanics with acoustic force spectroscopy |
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probing cellular mechanics with acoustic force spectroscopy |
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Cell Biology Molecular Biology |
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http://dx.doi.org/10.1091/mbc.e18-03-0154 |
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2018 |
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2005-2011 |
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<jats:p>A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.</jats:p> |
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| author | Sorkin, Raya, Bergamaschi, Giulia, Kamsma, Douwe, Brand, Guy, Dekel, Elya, Ofir-Birin, Yifat, Rudik, Ariel, Gironella, Marta, Ritort, Felix, Regev-Rudzki, Neta, Roos, Wouter H., Wuite, Gijs J. L. |
| author_facet | Sorkin, Raya, Bergamaschi, Giulia, Kamsma, Douwe, Brand, Guy, Dekel, Elya, Ofir-Birin, Yifat, Rudik, Ariel, Gironella, Marta, Ritort, Felix, Regev-Rudzki, Neta, Roos, Wouter H., Wuite, Gijs J. L., Sorkin, Raya, Bergamaschi, Giulia, Kamsma, Douwe, Brand, Guy, Dekel, Elya, Ofir-Birin, Yifat, Rudik, Ariel, Gironella, Marta, Ritort, Felix, Regev-Rudzki, Neta, Roos, Wouter H., Wuite, Gijs J. L. |
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| description | <jats:p>A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.</jats:p> |
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| spelling | Sorkin, Raya Bergamaschi, Giulia Kamsma, Douwe Brand, Guy Dekel, Elya Ofir-Birin, Yifat Rudik, Ariel Gironella, Marta Ritort, Felix Regev-Rudzki, Neta Roos, Wouter H. Wuite, Gijs J. L. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e18-03-0154 <jats:p>A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed single-cell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.</jats:p> Probing cellular mechanics with acoustic force spectroscopy Molecular Biology of the Cell |
| spellingShingle | Sorkin, Raya, Bergamaschi, Giulia, Kamsma, Douwe, Brand, Guy, Dekel, Elya, Ofir-Birin, Yifat, Rudik, Ariel, Gironella, Marta, Ritort, Felix, Regev-Rudzki, Neta, Roos, Wouter H., Wuite, Gijs J. L., Molecular Biology of the Cell, Probing cellular mechanics with acoustic force spectroscopy, Cell Biology, Molecular Biology |
| title | Probing cellular mechanics with acoustic force spectroscopy |
| title_full | Probing cellular mechanics with acoustic force spectroscopy |
| title_fullStr | Probing cellular mechanics with acoustic force spectroscopy |
| title_full_unstemmed | Probing cellular mechanics with acoustic force spectroscopy |
| title_short | Probing cellular mechanics with acoustic force spectroscopy |
| title_sort | probing cellular mechanics with acoustic force spectroscopy |
| title_unstemmed | Probing cellular mechanics with acoustic force spectroscopy |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e18-03-0154 |