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Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Biology of the Cell, 28, 2017, 14, S. 1997-2009 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. |
|---|---|
| author |
Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. |
| spellingShingle |
Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. Molecular Biology of the Cell Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression Cell Biology Molecular Biology |
| author_sort |
wang, yejun |
| spelling |
Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e16-12-0825 <jats:p> Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases. </jats:p> Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression Molecular Biology of the Cell |
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Molecular Biology of the Cell |
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| title |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_unstemmed |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_full |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_fullStr |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_full_unstemmed |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_short |
Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_sort |
orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| topic |
Cell Biology Molecular Biology |
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http://dx.doi.org/10.1091/mbc.e16-12-0825 |
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2017 |
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1997-2009 |
| description |
<jats:p> Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases. </jats:p> |
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| author | Wang, Yejun, Nagarajan, Mallika, Uhler, Caroline, Shivashankar, G. V. |
| author_facet | Wang, Yejun, Nagarajan, Mallika, Uhler, Caroline, Shivashankar, G. V., Wang, Yejun, Nagarajan, Mallika, Uhler, Caroline, Shivashankar, G. V. |
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| container_title | Molecular Biology of the Cell |
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| description | <jats:p> Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases. </jats:p> |
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| spelling | Wang, Yejun Nagarajan, Mallika Uhler, Caroline Shivashankar, G. V. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e16-12-0825 <jats:p> Extracellular matrix signals from the microenvironment regulate gene expression patterns and cell behavior. Using a combination of experiments and geometric models, we demonstrate correlations between cell geometry, three-dimensional (3D) organization of chromosome territories, and gene expression. Fluorescence in situ hybridization experiments showed that micropatterned fibroblasts cultured on anisotropic versus isotropic substrates resulted in repositioning of specific chromosomes, which contained genes that were differentially regulated by cell geometries. Experiments combined with ellipsoid packing models revealed that the mechanosensitivity of chromosomes was correlated with their orientation in the nucleus. Transcription inhibition experiments suggested that the intermingling degree was more sensitive to global changes in transcription than to chromosome radial positioning and its orientations. These results suggested that cell geometry modulated 3D chromosome arrangement, and their neighborhoods correlated with gene expression patterns in a predictable manner. This is central to understanding geometric control of genetic programs involved in cellular homeostasis and the associated diseases. </jats:p> Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression Molecular Biology of the Cell |
| spellingShingle | Wang, Yejun, Nagarajan, Mallika, Uhler, Caroline, Shivashankar, G. V., Molecular Biology of the Cell, Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression, Cell Biology, Molecular Biology |
| title | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_full | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_fullStr | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_full_unstemmed | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_short | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_sort | orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| title_unstemmed | Orientation and repositioning of chromosomes correlate with cell geometry–dependent gene expression |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e16-12-0825 |