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Cordon bleu promotes the assembly of brush border microvilli
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Biology of the Cell, 26, 2015, 21, S. 3803-3815 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. |
|---|---|
| author |
Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. |
| spellingShingle |
Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. Molecular Biology of the Cell Cordon bleu promotes the assembly of brush border microvilli Cell Biology Molecular Biology |
| author_sort |
grega-larson, nathan e. |
| spelling |
Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e15-06-0443 <jats:p> Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with the external environment. In the intestinal tract, these protrusions play central roles in nutrient absorption and host defense and are therefore essential for maintaining homeostasis. However, the mechanisms controlling microvillar assembly remain poorly understood. Here we report that the multifunctional actin regulator cordon bleu (COBL) promotes the growth of brush border (BB) microvilli. COBL localizes to the base of BB microvilli via a mechanism that requires its proline-rich N-terminus. Knockdown and overexpression studies show that COBL is needed for BB assembly and sufficient to induce microvillar growth using a mechanism that requires functional WH2 domains. We also find that COBL acts downstream of the F-BAR protein syndapin-2, which drives COBL targeting to the apical domain. These results provide insight into a mechanism that regulates microvillar growth during epithelial differentiation and have significant implications for understanding the maintenance of intestinal homeostasis. </jats:p> Cordon bleu promotes the assembly of brush border microvilli Molecular Biology of the Cell |
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| title |
Cordon bleu promotes the assembly of brush border microvilli |
| title_unstemmed |
Cordon bleu promotes the assembly of brush border microvilli |
| title_full |
Cordon bleu promotes the assembly of brush border microvilli |
| title_fullStr |
Cordon bleu promotes the assembly of brush border microvilli |
| title_full_unstemmed |
Cordon bleu promotes the assembly of brush border microvilli |
| title_short |
Cordon bleu promotes the assembly of brush border microvilli |
| title_sort |
cordon bleu promotes the assembly of brush border microvilli |
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Cell Biology Molecular Biology |
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http://dx.doi.org/10.1091/mbc.e15-06-0443 |
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2015 |
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3803-3815 |
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<jats:p> Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with the external environment. In the intestinal tract, these protrusions play central roles in nutrient absorption and host defense and are therefore essential for maintaining homeostasis. However, the mechanisms controlling microvillar assembly remain poorly understood. Here we report that the multifunctional actin regulator cordon bleu (COBL) promotes the growth of brush border (BB) microvilli. COBL localizes to the base of BB microvilli via a mechanism that requires its proline-rich N-terminus. Knockdown and overexpression studies show that COBL is needed for BB assembly and sufficient to induce microvillar growth using a mechanism that requires functional WH2 domains. We also find that COBL acts downstream of the F-BAR protein syndapin-2, which drives COBL targeting to the apical domain. These results provide insight into a mechanism that regulates microvillar growth during epithelial differentiation and have significant implications for understanding the maintenance of intestinal homeostasis. </jats:p> |
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| author | Grega-Larson, Nathan E., Crawley, Scott W., Erwin, Amanda L., Tyska, Matthew J. |
| author_facet | Grega-Larson, Nathan E., Crawley, Scott W., Erwin, Amanda L., Tyska, Matthew J., Grega-Larson, Nathan E., Crawley, Scott W., Erwin, Amanda L., Tyska, Matthew J. |
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| description | <jats:p> Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with the external environment. In the intestinal tract, these protrusions play central roles in nutrient absorption and host defense and are therefore essential for maintaining homeostasis. However, the mechanisms controlling microvillar assembly remain poorly understood. Here we report that the multifunctional actin regulator cordon bleu (COBL) promotes the growth of brush border (BB) microvilli. COBL localizes to the base of BB microvilli via a mechanism that requires its proline-rich N-terminus. Knockdown and overexpression studies show that COBL is needed for BB assembly and sufficient to induce microvillar growth using a mechanism that requires functional WH2 domains. We also find that COBL acts downstream of the F-BAR protein syndapin-2, which drives COBL targeting to the apical domain. These results provide insight into a mechanism that regulates microvillar growth during epithelial differentiation and have significant implications for understanding the maintenance of intestinal homeostasis. </jats:p> |
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| spelling | Grega-Larson, Nathan E. Crawley, Scott W. Erwin, Amanda L. Tyska, Matthew J. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e15-06-0443 <jats:p> Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with the external environment. In the intestinal tract, these protrusions play central roles in nutrient absorption and host defense and are therefore essential for maintaining homeostasis. However, the mechanisms controlling microvillar assembly remain poorly understood. Here we report that the multifunctional actin regulator cordon bleu (COBL) promotes the growth of brush border (BB) microvilli. COBL localizes to the base of BB microvilli via a mechanism that requires its proline-rich N-terminus. Knockdown and overexpression studies show that COBL is needed for BB assembly and sufficient to induce microvillar growth using a mechanism that requires functional WH2 domains. We also find that COBL acts downstream of the F-BAR protein syndapin-2, which drives COBL targeting to the apical domain. These results provide insight into a mechanism that regulates microvillar growth during epithelial differentiation and have significant implications for understanding the maintenance of intestinal homeostasis. </jats:p> Cordon bleu promotes the assembly of brush border microvilli Molecular Biology of the Cell |
| spellingShingle | Grega-Larson, Nathan E., Crawley, Scott W., Erwin, Amanda L., Tyska, Matthew J., Molecular Biology of the Cell, Cordon bleu promotes the assembly of brush border microvilli, Cell Biology, Molecular Biology |
| title | Cordon bleu promotes the assembly of brush border microvilli |
| title_full | Cordon bleu promotes the assembly of brush border microvilli |
| title_fullStr | Cordon bleu promotes the assembly of brush border microvilli |
| title_full_unstemmed | Cordon bleu promotes the assembly of brush border microvilli |
| title_short | Cordon bleu promotes the assembly of brush border microvilli |
| title_sort | cordon bleu promotes the assembly of brush border microvilli |
| title_unstemmed | Cordon bleu promotes the assembly of brush border microvilli |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e15-06-0443 |