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Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Biology of the Cell, 17, 2006, 2, S. 907-916 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero |
|---|---|
| author |
Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero |
| spellingShingle |
Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero Molecular Biology of the Cell Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo Cell Biology Molecular Biology |
| author_sort |
straube, anne |
| spelling |
Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e05-06-0542 <jats:p>Conventional kinesin is a ubiquitous organelle transporter that moves cargo toward the plus-ends of microtubules. In addition, several in vitro studies indicated a role of conventional kinesin in cross-bridging and sliding microtubules, but in vivo evidence for such a role is missing. In this study, we show that conventional kinesin mediates microtubule-microtubule interactions in the model fungus Ustilago maydis. Live cell imaging and ultrastructural analysis of various mutants in Kin1 revealed that this kinesin-1 motor is required for efficient microtubule bundling and participates in microtubule bending in vivo. High levels of Kin1 led to increased microtubule bending, whereas a rigor-mutation in the motor head suppressed all microtubule motility and promoted strong microtubule bundling, indicating that kinesin can form cross-bridges between microtubules in living cells. This effect required a conserved region in the C terminus of Kin1, which was shown to bind microtubules in vitro. In addition, a fusion protein of yellow fluorescent protein and the Kin1tail localized to microtubule bundles, further supporting the idea that a conserved microtubule binding activity in the tail of conventional kinesins mediates microtubule-microtubule interactions in vivo.</jats:p> Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo Molecular Biology of the Cell |
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| title |
Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_unstemmed |
Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_full |
Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
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Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_full_unstemmed |
Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_short |
Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_sort |
conventional kinesin mediates microtubule-microtubule interactions in vivo |
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Cell Biology Molecular Biology |
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http://dx.doi.org/10.1091/mbc.e05-06-0542 |
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2006 |
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907-916 |
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<jats:p>Conventional kinesin is a ubiquitous organelle transporter that moves cargo toward the plus-ends of microtubules. In addition, several in vitro studies indicated a role of conventional kinesin in cross-bridging and sliding microtubules, but in vivo evidence for such a role is missing. In this study, we show that conventional kinesin mediates microtubule-microtubule interactions in the model fungus Ustilago maydis. Live cell imaging and ultrastructural analysis of various mutants in Kin1 revealed that this kinesin-1 motor is required for efficient microtubule bundling and participates in microtubule bending in vivo. High levels of Kin1 led to increased microtubule bending, whereas a rigor-mutation in the motor head suppressed all microtubule motility and promoted strong microtubule bundling, indicating that kinesin can form cross-bridges between microtubules in living cells. This effect required a conserved region in the C terminus of Kin1, which was shown to bind microtubules in vitro. In addition, a fusion protein of yellow fluorescent protein and the Kin1tail localized to microtubule bundles, further supporting the idea that a conserved microtubule binding activity in the tail of conventional kinesins mediates microtubule-microtubule interactions in vivo.</jats:p> |
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| author | Straube, Anne, Hause, Gerd, Fink, Gero, Steinberg, Gero |
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| description | <jats:p>Conventional kinesin is a ubiquitous organelle transporter that moves cargo toward the plus-ends of microtubules. In addition, several in vitro studies indicated a role of conventional kinesin in cross-bridging and sliding microtubules, but in vivo evidence for such a role is missing. In this study, we show that conventional kinesin mediates microtubule-microtubule interactions in the model fungus Ustilago maydis. Live cell imaging and ultrastructural analysis of various mutants in Kin1 revealed that this kinesin-1 motor is required for efficient microtubule bundling and participates in microtubule bending in vivo. High levels of Kin1 led to increased microtubule bending, whereas a rigor-mutation in the motor head suppressed all microtubule motility and promoted strong microtubule bundling, indicating that kinesin can form cross-bridges between microtubules in living cells. This effect required a conserved region in the C terminus of Kin1, which was shown to bind microtubules in vitro. In addition, a fusion protein of yellow fluorescent protein and the Kin1tail localized to microtubule bundles, further supporting the idea that a conserved microtubule binding activity in the tail of conventional kinesins mediates microtubule-microtubule interactions in vivo.</jats:p> |
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| spelling | Straube, Anne Hause, Gerd Fink, Gero Steinberg, Gero 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e05-06-0542 <jats:p>Conventional kinesin is a ubiquitous organelle transporter that moves cargo toward the plus-ends of microtubules. In addition, several in vitro studies indicated a role of conventional kinesin in cross-bridging and sliding microtubules, but in vivo evidence for such a role is missing. In this study, we show that conventional kinesin mediates microtubule-microtubule interactions in the model fungus Ustilago maydis. Live cell imaging and ultrastructural analysis of various mutants in Kin1 revealed that this kinesin-1 motor is required for efficient microtubule bundling and participates in microtubule bending in vivo. High levels of Kin1 led to increased microtubule bending, whereas a rigor-mutation in the motor head suppressed all microtubule motility and promoted strong microtubule bundling, indicating that kinesin can form cross-bridges between microtubules in living cells. This effect required a conserved region in the C terminus of Kin1, which was shown to bind microtubules in vitro. In addition, a fusion protein of yellow fluorescent protein and the Kin1tail localized to microtubule bundles, further supporting the idea that a conserved microtubule binding activity in the tail of conventional kinesins mediates microtubule-microtubule interactions in vivo.</jats:p> Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo Molecular Biology of the Cell |
| spellingShingle | Straube, Anne, Hause, Gerd, Fink, Gero, Steinberg, Gero, Molecular Biology of the Cell, Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo, Cell Biology, Molecular Biology |
| title | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_full | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_fullStr | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_full_unstemmed | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_short | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| title_sort | conventional kinesin mediates microtubule-microtubule interactions in vivo |
| title_unstemmed | Conventional Kinesin Mediates Microtubule-Microtubule Interactions In Vivo |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e05-06-0542 |