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Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin
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| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Biology of the Cell, 9, 1998, 5, S. 1163-1176 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. |
|---|---|
| author |
Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. |
| spellingShingle |
Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. Molecular Biology of the Cell Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin Cell Biology Molecular Biology |
| author_sort |
lu, zhi hong |
| spelling |
Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.9.5.1163 <jats:p>Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopussperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.</jats:p> Histone H1 Reduces the Frequency of Initiation in<i>Xenopus</i>Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin Molecular Biology of the Cell |
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| title |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_unstemmed |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_full |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_fullStr |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_full_unstemmed |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_short |
Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_sort |
histone h1 reduces the frequency of initiation in<i>xenopus</i>egg extract by limiting the assembly of prereplication complexes on sperm chromatin |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1091/mbc.9.5.1163 |
| publishDate |
1998 |
| physical |
1163-1176 |
| description |
<jats:p>Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopussperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.</jats:p> |
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| author | Lu, Zhi Hong, Sittman, Donald B., Romanowski, Piotr, Leno, Gregory H. |
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| description | <jats:p>Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopussperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.</jats:p> |
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| physical | 1163-1176 |
| publishDate | 1998 |
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| spelling | Lu, Zhi Hong Sittman, Donald B. Romanowski, Piotr Leno, Gregory H. 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.9.5.1163 <jats:p>Somatic histone H1 reduces both the rate and extent of DNA replication in Xenopus egg extract. We show here that H1 inhibits replication directly by reducing the number of replication forks, but not the rate of fork progression, in Xenopussperm nuclei. Density substitution experiments demonstrate that those forks that are active in H1 nuclei elongate to form large tracts of fully replicated DNA, indicating that inhibition is due to a reduction in the frequency of initiation and not the rate or extent of elongation. The observation that H1 dramatically reduces the number of replication foci in sperm nuclei supports this view. The establishment of replication competent DNA in egg extract requires the assembly of prereplication complexes (pre-RCs) on sperm chromatin. H1 reduces binding of the pre-RC proteins, XOrc2, XCdc6, and XMcm3, to chromatin. Replication competence can be restored in these nuclei, however, only under conditions that promote the loss of H1 from chromatin and licensing of the DNA. Thus, H1 inhibits replication in egg extract by preventing the assembly of pre-RCs on sperm chromatin, thereby reducing the frequency of initiation. These data raise the interesting possibility that H1 plays a role in regulating replication origin use during Xenopus development.</jats:p> Histone H1 Reduces the Frequency of Initiation in<i>Xenopus</i>Egg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin Molecular Biology of the Cell |
| spellingShingle | Lu, Zhi Hong, Sittman, Donald B., Romanowski, Piotr, Leno, Gregory H., Molecular Biology of the Cell, Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin, Cell Biology, Molecular Biology |
| title | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_full | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_fullStr | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_full_unstemmed | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_short | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| title_sort | histone h1 reduces the frequency of initiation in<i>xenopus</i>egg extract by limiting the assembly of prereplication complexes on sperm chromatin |
| title_unstemmed | Histone H1 Reduces the Frequency of Initiation inXenopusEgg Extract by Limiting the Assembly of Prereplication Complexes on Sperm Chromatin |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.9.5.1163 |