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Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes.
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| Zeitschriftentitel: | The Journal of experimental medicine |
|---|---|
| Personen und Körperschaften: | , , |
| In: | The Journal of experimental medicine, 170, 1989, 6, S. 1987-1998 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Louie, M C Nelson, C A Loh, D Y Louie, M C Nelson, C A Loh, D Y |
|---|---|
| author |
Louie, M C Nelson, C A Loh, D Y |
| spellingShingle |
Louie, M C Nelson, C A Loh, D Y The Journal of experimental medicine Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. Immunology Immunology and Allergy |
| author_sort |
louie, m c |
| spelling |
Louie, M C Nelson, C A Loh, D Y 0022-1007 1540-9538 Rockefeller University Press Immunology Immunology and Allergy http://dx.doi.org/10.1084/jem.170.6.1987 <jats:p>By screening previously isolated genomic clones spanning the mouse TCR V beta locus with V beta-specific oligonucleotides, we have isolated one new functional V beta gene and six V beta pseudogenes. Because this method of identifying new genes does not depend on expression levels, we conclude that most, if not all, V beta genes in the mouse have been identified. The newly identified pseudogenes increase the frequency of mouse TCR V beta pseudogenes to 28%, a frequency similar to that estimated for mouse Ig VH pseudogenes (24). Three of the newly discovered pseudogenes are clustered in a region around another pseudogene (V beta 17b). The extensive DNA diversity, as reflected in both the nucleotide sequence and the RFLP, indicates that this genomic region is a possible hotspot of recombination. The new functional gene, V beta 19a, is expressed at very low levels, which explains why it has not been isolated earlier. V beta 19 shows expression patterns that correlate with the previously described Va beta and Vb beta haplotypes.</jats:p> Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. The Journal of experimental medicine |
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Rockefeller University Press, 1989 |
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The Journal of experimental medicine |
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| title |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_unstemmed |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_full |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_fullStr |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_full_unstemmed |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_short |
Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_sort |
identification and characterization of new murine t cell receptor beta chain variable region (v beta) genes. |
| topic |
Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1084/jem.170.6.1987 |
| publishDate |
1989 |
| physical |
1987-1998 |
| description |
<jats:p>By screening previously isolated genomic clones spanning the mouse TCR V beta locus with V beta-specific oligonucleotides, we have isolated one new functional V beta gene and six V beta pseudogenes. Because this method of identifying new genes does not depend on expression levels, we conclude that most, if not all, V beta genes in the mouse have been identified. The newly identified pseudogenes increase the frequency of mouse TCR V beta pseudogenes to 28%, a frequency similar to that estimated for mouse Ig VH pseudogenes (24). Three of the newly discovered pseudogenes are clustered in a region around another pseudogene (V beta 17b). The extensive DNA diversity, as reflected in both the nucleotide sequence and the RFLP, indicates that this genomic region is a possible hotspot of recombination. The new functional gene, V beta 19a, is expressed at very low levels, which explains why it has not been isolated earlier. V beta 19 shows expression patterns that correlate with the previously described Va beta and Vb beta haplotypes.</jats:p> |
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| author | Louie, M C, Nelson, C A, Loh, D Y |
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| author_sort | louie, m c |
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| container_title | The Journal of experimental medicine |
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| description | <jats:p>By screening previously isolated genomic clones spanning the mouse TCR V beta locus with V beta-specific oligonucleotides, we have isolated one new functional V beta gene and six V beta pseudogenes. Because this method of identifying new genes does not depend on expression levels, we conclude that most, if not all, V beta genes in the mouse have been identified. The newly identified pseudogenes increase the frequency of mouse TCR V beta pseudogenes to 28%, a frequency similar to that estimated for mouse Ig VH pseudogenes (24). Three of the newly discovered pseudogenes are clustered in a region around another pseudogene (V beta 17b). The extensive DNA diversity, as reflected in both the nucleotide sequence and the RFLP, indicates that this genomic region is a possible hotspot of recombination. The new functional gene, V beta 19a, is expressed at very low levels, which explains why it has not been isolated earlier. V beta 19 shows expression patterns that correlate with the previously described Va beta and Vb beta haplotypes.</jats:p> |
| doi_str_mv | 10.1084/jem.170.6.1987 |
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| imprint | Rockefeller University Press, 1989 |
| imprint_str_mv | Rockefeller University Press, 1989 |
| institution | DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15 |
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| physical | 1987-1998 |
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| series | The Journal of experimental medicine |
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| spelling | Louie, M C Nelson, C A Loh, D Y 0022-1007 1540-9538 Rockefeller University Press Immunology Immunology and Allergy http://dx.doi.org/10.1084/jem.170.6.1987 <jats:p>By screening previously isolated genomic clones spanning the mouse TCR V beta locus with V beta-specific oligonucleotides, we have isolated one new functional V beta gene and six V beta pseudogenes. Because this method of identifying new genes does not depend on expression levels, we conclude that most, if not all, V beta genes in the mouse have been identified. The newly identified pseudogenes increase the frequency of mouse TCR V beta pseudogenes to 28%, a frequency similar to that estimated for mouse Ig VH pseudogenes (24). Three of the newly discovered pseudogenes are clustered in a region around another pseudogene (V beta 17b). The extensive DNA diversity, as reflected in both the nucleotide sequence and the RFLP, indicates that this genomic region is a possible hotspot of recombination. The new functional gene, V beta 19a, is expressed at very low levels, which explains why it has not been isolated earlier. V beta 19 shows expression patterns that correlate with the previously described Va beta and Vb beta haplotypes.</jats:p> Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. The Journal of experimental medicine |
| spellingShingle | Louie, M C, Nelson, C A, Loh, D Y, The Journal of experimental medicine, Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes., Immunology, Immunology and Allergy |
| title | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_full | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_fullStr | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_full_unstemmed | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_short | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| title_sort | identification and characterization of new murine t cell receptor beta chain variable region (v beta) genes. |
| title_unstemmed | Identification and characterization of new murine T cell receptor beta chain variable region (V beta) genes. |
| topic | Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1084/jem.170.6.1987 |