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Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide.
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| Zeitschriftentitel: | The Journal of experimental medicine |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | The Journal of experimental medicine, 177, 1993, 1, S. 89-97 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
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| Schlagwörter: |
| author_facet |
Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M |
|---|---|
| author |
Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M |
| spellingShingle |
Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M The Journal of experimental medicine Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. Immunology Immunology and Allergy |
| author_sort |
warren, h s |
| spelling |
Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M 0022-1007 1540-9538 Rockefeller University Press Immunology Immunology and Allergy http://dx.doi.org/10.1084/jem.177.1.89 <jats:p>The use of monoclonal antibodies (mAbs) directed to lipid A for the therapy of gram-negative sepsis is controversial. In an attempt to understand their biologic basis of action, we used a fluid-phase radioimmunoassay to measure binding between bacterial lipopolysaccharide (LPS) and two IgM mAbs directed to lipid A that are being evaluated for the treatment of gram-negative bacterial sepsis. Both antibodies bound 3H-LPS prepared from multiple strains of gram-negative bacteria when large excesses of antibody were used, although binding was modest and only slightly greater than control preparations. We also studied the ability of each anti-lipid A antibody to neutralize some of the biological effects of LPS in vitro. Despite large molar excesses, neither antibody neutralized LPS as assessed by the limulus lysate test, by a mitogenic assay for murine splenocytes, or by the production of cytokines interleukin (IL)-1, IL-6, or tumor necrosis factor from human monocytes in culture medium or in whole blood. Our experiments do not support the hypothesis that either of these anti-lipid A mAbs function by neutralizing the toxic effects of LPS.</jats:p> Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. The Journal of experimental medicine |
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| title |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_unstemmed |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_full |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_fullStr |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_full_unstemmed |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_short |
Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_sort |
assessment of ability of murine and human anti-lipid a monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| topic |
Immunology Immunology and Allergy |
| url |
http://dx.doi.org/10.1084/jem.177.1.89 |
| publishDate |
1993 |
| physical |
89-97 |
| description |
<jats:p>The use of monoclonal antibodies (mAbs) directed to lipid A for the therapy of gram-negative sepsis is controversial. In an attempt to understand their biologic basis of action, we used a fluid-phase radioimmunoassay to measure binding between bacterial lipopolysaccharide (LPS) and two IgM mAbs directed to lipid A that are being evaluated for the treatment of gram-negative bacterial sepsis. Both antibodies bound 3H-LPS prepared from multiple strains of gram-negative bacteria when large excesses of antibody were used, although binding was modest and only slightly greater than control preparations. We also studied the ability of each anti-lipid A antibody to neutralize some of the biological effects of LPS in vitro. Despite large molar excesses, neither antibody neutralized LPS as assessed by the limulus lysate test, by a mitogenic assay for murine splenocytes, or by the production of cytokines interleukin (IL)-1, IL-6, or tumor necrosis factor from human monocytes in culture medium or in whole blood. Our experiments do not support the hypothesis that either of these anti-lipid A mAbs function by neutralizing the toxic effects of LPS.</jats:p> |
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| author | Warren, H S, Amato, S F, Fitting, C, Black, K M, Loiselle, P M, Pasternack, M S, Cavaillon, J M |
| author_facet | Warren, H S, Amato, S F, Fitting, C, Black, K M, Loiselle, P M, Pasternack, M S, Cavaillon, J M, Warren, H S, Amato, S F, Fitting, C, Black, K M, Loiselle, P M, Pasternack, M S, Cavaillon, J M |
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| description | <jats:p>The use of monoclonal antibodies (mAbs) directed to lipid A for the therapy of gram-negative sepsis is controversial. In an attempt to understand their biologic basis of action, we used a fluid-phase radioimmunoassay to measure binding between bacterial lipopolysaccharide (LPS) and two IgM mAbs directed to lipid A that are being evaluated for the treatment of gram-negative bacterial sepsis. Both antibodies bound 3H-LPS prepared from multiple strains of gram-negative bacteria when large excesses of antibody were used, although binding was modest and only slightly greater than control preparations. We also studied the ability of each anti-lipid A antibody to neutralize some of the biological effects of LPS in vitro. Despite large molar excesses, neither antibody neutralized LPS as assessed by the limulus lysate test, by a mitogenic assay for murine splenocytes, or by the production of cytokines interleukin (IL)-1, IL-6, or tumor necrosis factor from human monocytes in culture medium or in whole blood. Our experiments do not support the hypothesis that either of these anti-lipid A mAbs function by neutralizing the toxic effects of LPS.</jats:p> |
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| spelling | Warren, H S Amato, S F Fitting, C Black, K M Loiselle, P M Pasternack, M S Cavaillon, J M 0022-1007 1540-9538 Rockefeller University Press Immunology Immunology and Allergy http://dx.doi.org/10.1084/jem.177.1.89 <jats:p>The use of monoclonal antibodies (mAbs) directed to lipid A for the therapy of gram-negative sepsis is controversial. In an attempt to understand their biologic basis of action, we used a fluid-phase radioimmunoassay to measure binding between bacterial lipopolysaccharide (LPS) and two IgM mAbs directed to lipid A that are being evaluated for the treatment of gram-negative bacterial sepsis. Both antibodies bound 3H-LPS prepared from multiple strains of gram-negative bacteria when large excesses of antibody were used, although binding was modest and only slightly greater than control preparations. We also studied the ability of each anti-lipid A antibody to neutralize some of the biological effects of LPS in vitro. Despite large molar excesses, neither antibody neutralized LPS as assessed by the limulus lysate test, by a mitogenic assay for murine splenocytes, or by the production of cytokines interleukin (IL)-1, IL-6, or tumor necrosis factor from human monocytes in culture medium or in whole blood. Our experiments do not support the hypothesis that either of these anti-lipid A mAbs function by neutralizing the toxic effects of LPS.</jats:p> Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. The Journal of experimental medicine |
| spellingShingle | Warren, H S, Amato, S F, Fitting, C, Black, K M, Loiselle, P M, Pasternack, M S, Cavaillon, J M, The Journal of experimental medicine, Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide., Immunology, Immunology and Allergy |
| title | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_full | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_fullStr | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_full_unstemmed | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_short | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_sort | assessment of ability of murine and human anti-lipid a monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| title_unstemmed | Assessment of ability of murine and human anti-lipid A monoclonal antibodies to bind and neutralize lipopolysaccharide. |
| topic | Immunology, Immunology and Allergy |
| url | http://dx.doi.org/10.1084/jem.177.1.89 |