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MAMMALIAN CHROMOSOMES IN VITRO
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| Zeitschriftentitel: | The Journal of Cell Biology |
|---|---|
| Personen und Körperschaften: | |
| In: | The Journal of Cell Biology, 23, 1964, 1, S. 53-62 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Hsu, T. C. Hsu, T. C. |
|---|---|
| author |
Hsu, T. C. |
| spellingShingle |
Hsu, T. C. The Journal of Cell Biology MAMMALIAN CHROMOSOMES IN VITRO Cell Biology |
| author_sort |
hsu, t. c. |
| spelling |
Hsu, T. C. 1540-8140 0021-9525 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.23.1.53 <jats:p>The complete DNA replication sequence of the entire complement of chromosomes in the Chinese hamster may be studied by using the method of continuous H3-thymidine labeling and the method of 5-fluorodeoxyuridine block with H3-thymidine pulse labeling as relief. Many chromosomes start DNA synthesis simultaneously at multiple sites, but the sex chromosomes (the Y and the long arm of the X) begin DNA replication approximately 4.5 hours later and are the last members of the complement to finish replication. Generally, chromosomes or segments of chromosomes that begin replication early complete it early, and those which begin late, complete it late. Many chromosomes bear characteristically late replicating regions. During the last hour of the S phase, the entire Y, the long arm of the X, and chromosomes 10 and 11 are heavily labeled. The short arm of chromosome 1, long arm of chromosome 2, distal portion of chromosome 6, and short arms of chromosomes 7, 8, and 9 are moderately labeled. The long arm of chromosome 1 and the short arm of chromosome 2 also have late replicating zones or bands. The centromeres of chromosomes 4 and 5, and occasionally a band on the short arm of the X are lightly labeled.</jats:p> MAMMALIAN CHROMOSOMES <i>IN VITRO </i> The Journal of Cell Biology |
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Rockefeller University Press, 1964 |
| imprint_str_mv |
Rockefeller University Press, 1964 |
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0021-9525 1540-8140 |
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0021-9525 1540-8140 |
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1964 |
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Rockefeller University Press |
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The Journal of Cell Biology |
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49 |
| title |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_unstemmed |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_full |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_fullStr |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_full_unstemmed |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_short |
MAMMALIAN CHROMOSOMES IN VITRO |
| title_sort |
mammalian chromosomes <i>in vitro </i> |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.23.1.53 |
| publishDate |
1964 |
| physical |
53-62 |
| description |
<jats:p>The complete DNA replication sequence of the entire complement of chromosomes in the Chinese hamster may be studied by using the method of continuous H3-thymidine labeling and the method of 5-fluorodeoxyuridine block with H3-thymidine pulse labeling as relief. Many chromosomes start DNA synthesis simultaneously at multiple sites, but the sex chromosomes (the Y and the long arm of the X) begin DNA replication approximately 4.5 hours later and are the last members of the complement to finish replication. Generally, chromosomes or segments of chromosomes that begin replication early complete it early, and those which begin late, complete it late. Many chromosomes bear characteristically late replicating regions. During the last hour of the S phase, the entire Y, the long arm of the X, and chromosomes 10 and 11 are heavily labeled. The short arm of chromosome 1, long arm of chromosome 2, distal portion of chromosome 6, and short arms of chromosomes 7, 8, and 9 are moderately labeled. The long arm of chromosome 1 and the short arm of chromosome 2 also have late replicating zones or bands. The centromeres of chromosomes 4 and 5, and occasionally a band on the short arm of the X are lightly labeled.</jats:p> |
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| description | <jats:p>The complete DNA replication sequence of the entire complement of chromosomes in the Chinese hamster may be studied by using the method of continuous H3-thymidine labeling and the method of 5-fluorodeoxyuridine block with H3-thymidine pulse labeling as relief. Many chromosomes start DNA synthesis simultaneously at multiple sites, but the sex chromosomes (the Y and the long arm of the X) begin DNA replication approximately 4.5 hours later and are the last members of the complement to finish replication. Generally, chromosomes or segments of chromosomes that begin replication early complete it early, and those which begin late, complete it late. Many chromosomes bear characteristically late replicating regions. During the last hour of the S phase, the entire Y, the long arm of the X, and chromosomes 10 and 11 are heavily labeled. The short arm of chromosome 1, long arm of chromosome 2, distal portion of chromosome 6, and short arms of chromosomes 7, 8, and 9 are moderately labeled. The long arm of chromosome 1 and the short arm of chromosome 2 also have late replicating zones or bands. The centromeres of chromosomes 4 and 5, and occasionally a band on the short arm of the X are lightly labeled.</jats:p> |
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| imprint | Rockefeller University Press, 1964 |
| imprint_str_mv | Rockefeller University Press, 1964 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0021-9525, 1540-8140 |
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| language | English |
| last_indexed | 2024-03-01T16:36:01.354Z |
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| physical | 53-62 |
| publishDate | 1964 |
| publishDateSort | 1964 |
| publisher | Rockefeller University Press |
| record_format | ai |
| recordtype | ai |
| series | The Journal of Cell Biology |
| source_id | 49 |
| spelling | Hsu, T. C. 1540-8140 0021-9525 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.23.1.53 <jats:p>The complete DNA replication sequence of the entire complement of chromosomes in the Chinese hamster may be studied by using the method of continuous H3-thymidine labeling and the method of 5-fluorodeoxyuridine block with H3-thymidine pulse labeling as relief. Many chromosomes start DNA synthesis simultaneously at multiple sites, but the sex chromosomes (the Y and the long arm of the X) begin DNA replication approximately 4.5 hours later and are the last members of the complement to finish replication. Generally, chromosomes or segments of chromosomes that begin replication early complete it early, and those which begin late, complete it late. Many chromosomes bear characteristically late replicating regions. During the last hour of the S phase, the entire Y, the long arm of the X, and chromosomes 10 and 11 are heavily labeled. The short arm of chromosome 1, long arm of chromosome 2, distal portion of chromosome 6, and short arms of chromosomes 7, 8, and 9 are moderately labeled. The long arm of chromosome 1 and the short arm of chromosome 2 also have late replicating zones or bands. The centromeres of chromosomes 4 and 5, and occasionally a band on the short arm of the X are lightly labeled.</jats:p> MAMMALIAN CHROMOSOMES <i>IN VITRO </i> The Journal of Cell Biology |
| spellingShingle | Hsu, T. C., The Journal of Cell Biology, MAMMALIAN CHROMOSOMES IN VITRO , Cell Biology |
| title | MAMMALIAN CHROMOSOMES IN VITRO |
| title_full | MAMMALIAN CHROMOSOMES IN VITRO |
| title_fullStr | MAMMALIAN CHROMOSOMES IN VITRO |
| title_full_unstemmed | MAMMALIAN CHROMOSOMES IN VITRO |
| title_short | MAMMALIAN CHROMOSOMES IN VITRO |
| title_sort | mammalian chromosomes <i>in vitro </i> |
| title_unstemmed | MAMMALIAN CHROMOSOMES IN VITRO |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.23.1.53 |