Eintrag weiter verarbeiten
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18).
Gespeichert in:
| Zeitschriftentitel: | The Journal of cell biology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | The Journal of cell biology, 111, 1990, 6, S. 3129-3139 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A |
|---|---|
| author |
Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A |
| spellingShingle |
Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A The Journal of cell biology ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). Cell Biology |
| author_sort |
diamond, m s |
| spelling |
Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.111.6.3129 <jats:p>While the leukocyte integrin lymphocyte function-associated antigen (LFA)-1 has been demonstrated to bind intercellular adhesion molecule (ICAM)-1, results with the related Mac-1 molecule have been controversial. We have used multiple cell binding assays, purified Mac-1 and ICAM-1, and cell lines transfected with Mac-1 and ICAM-1 cDNAs to examine the interaction of ICAM-1 with Mac-1. Stimulated human umbilical vein endothelial cells (HUVECs), which express a high surface density of ICAM-1, bind to immunoaffinity-purified Mac-1 adsorbed to artificial substrates in a manner that is inhibited by mAbs to Mac-1 and ICAM-1. Transfected murine L cells or monkey COS cells expressing human ICAM-1 bind to purified Mac-1 in a specific and dose-dependent manner; the attachment to Mac-1 is more temperature sensitive, lower in avidity, and blocked by a different series of ICAM-1 mAbs when compared to LFA-1. In a reciprocal assay, COS cells cotransfected with the alpha and beta chain cDNAs of Mac-1 or LFA-1 attach to immunoaffinity-purified ICAM-1 substrates; this adhesion is blocked by mAbs to ICAM-1 and Mac-1 or LFA-1. Two color fluorescence cell conjugate experiments show that neutrophils stimulated with fMLP bind to HUVEC stimulated with lipopolysaccharide for 24 h in an ICAM-1-, Mac-1-, and LFA-1-dependent fashion. Because cellular and purified Mac-1 interact with cellular and purified ICAM-1, we conclude that ICAM-1 is a counter receptor for Mac-1 and that this receptor pair is responsible, in part, for the adhesion between stimulated neutrophils and stimulated endothelial cells.</jats:p> ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). The Journal of cell biology |
| doi_str_mv |
10.1083/jcb.111.6.3129 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTExLjYuMzEyOQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTExLjYuMzEyOQ |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 |
| imprint |
Rockefeller University Press, 1990 |
| imprint_str_mv |
Rockefeller University Press, 1990 |
| issn |
0021-9525 1540-8140 |
| issn_str_mv |
0021-9525 1540-8140 |
| language |
English |
| mega_collection |
Rockefeller University Press (CrossRef) |
| match_str |
diamond1990icam1cd54acounterreceptorformac1cd11bcd18 |
| publishDateSort |
1990 |
| publisher |
Rockefeller University Press |
| recordtype |
ai |
| record_format |
ai |
| series |
The Journal of cell biology |
| source_id |
49 |
| title |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_unstemmed |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_full |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_fullStr |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_full_unstemmed |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_short |
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_sort |
icam-1 (cd54): a counter-receptor for mac-1 (cd11b/cd18). |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.111.6.3129 |
| publishDate |
1990 |
| physical |
3129-3139 |
| description |
<jats:p>While the leukocyte integrin lymphocyte function-associated antigen (LFA)-1 has been demonstrated to bind intercellular adhesion molecule (ICAM)-1, results with the related Mac-1 molecule have been controversial. We have used multiple cell binding assays, purified Mac-1 and ICAM-1, and cell lines transfected with Mac-1 and ICAM-1 cDNAs to examine the interaction of ICAM-1 with Mac-1. Stimulated human umbilical vein endothelial cells (HUVECs), which express a high surface density of ICAM-1, bind to immunoaffinity-purified Mac-1 adsorbed to artificial substrates in a manner that is inhibited by mAbs to Mac-1 and ICAM-1. Transfected murine L cells or monkey COS cells expressing human ICAM-1 bind to purified Mac-1 in a specific and dose-dependent manner; the attachment to Mac-1 is more temperature sensitive, lower in avidity, and blocked by a different series of ICAM-1 mAbs when compared to LFA-1. In a reciprocal assay, COS cells cotransfected with the alpha and beta chain cDNAs of Mac-1 or LFA-1 attach to immunoaffinity-purified ICAM-1 substrates; this adhesion is blocked by mAbs to ICAM-1 and Mac-1 or LFA-1. Two color fluorescence cell conjugate experiments show that neutrophils stimulated with fMLP bind to HUVEC stimulated with lipopolysaccharide for 24 h in an ICAM-1-, Mac-1-, and LFA-1-dependent fashion. Because cellular and purified Mac-1 interact with cellular and purified ICAM-1, we conclude that ICAM-1 is a counter receptor for Mac-1 and that this receptor pair is responsible, in part, for the adhesion between stimulated neutrophils and stimulated endothelial cells.</jats:p> |
| container_issue |
6 |
| container_start_page |
3129 |
| container_title |
The Journal of cell biology |
| container_volume |
111 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792346846016831493 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:45:52.106Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=ICAM-1+%28CD54%29%3A+a+counter-receptor+for+Mac-1+%28CD11b%2FCD18%29.&rft.date=1990-12-01&genre=article&issn=1540-8140&volume=111&issue=6&spage=3129&epage=3139&pages=3129-3139&jtitle=The+Journal+of+cell+biology&atitle=ICAM-1+%28CD54%29%3A+a+counter-receptor+for+Mac-1+%28CD11b%2FCD18%29.&aulast=Springer&aufirst=T+A&rft_id=info%3Adoi%2F10.1083%2Fjcb.111.6.3129&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792346846016831493 |
| author | Diamond, M S, Staunton, D E, de Fougerolles, A R, Stacker, S A, Garcia-Aguilar, J, Hibbs, M L, Springer, T A |
| author_facet | Diamond, M S, Staunton, D E, de Fougerolles, A R, Stacker, S A, Garcia-Aguilar, J, Hibbs, M L, Springer, T A, Diamond, M S, Staunton, D E, de Fougerolles, A R, Stacker, S A, Garcia-Aguilar, J, Hibbs, M L, Springer, T A |
| author_sort | diamond, m s |
| container_issue | 6 |
| container_start_page | 3129 |
| container_title | The Journal of cell biology |
| container_volume | 111 |
| description | <jats:p>While the leukocyte integrin lymphocyte function-associated antigen (LFA)-1 has been demonstrated to bind intercellular adhesion molecule (ICAM)-1, results with the related Mac-1 molecule have been controversial. We have used multiple cell binding assays, purified Mac-1 and ICAM-1, and cell lines transfected with Mac-1 and ICAM-1 cDNAs to examine the interaction of ICAM-1 with Mac-1. Stimulated human umbilical vein endothelial cells (HUVECs), which express a high surface density of ICAM-1, bind to immunoaffinity-purified Mac-1 adsorbed to artificial substrates in a manner that is inhibited by mAbs to Mac-1 and ICAM-1. Transfected murine L cells or monkey COS cells expressing human ICAM-1 bind to purified Mac-1 in a specific and dose-dependent manner; the attachment to Mac-1 is more temperature sensitive, lower in avidity, and blocked by a different series of ICAM-1 mAbs when compared to LFA-1. In a reciprocal assay, COS cells cotransfected with the alpha and beta chain cDNAs of Mac-1 or LFA-1 attach to immunoaffinity-purified ICAM-1 substrates; this adhesion is blocked by mAbs to ICAM-1 and Mac-1 or LFA-1. Two color fluorescence cell conjugate experiments show that neutrophils stimulated with fMLP bind to HUVEC stimulated with lipopolysaccharide for 24 h in an ICAM-1-, Mac-1-, and LFA-1-dependent fashion. Because cellular and purified Mac-1 interact with cellular and purified ICAM-1, we conclude that ICAM-1 is a counter receptor for Mac-1 and that this receptor pair is responsible, in part, for the adhesion between stimulated neutrophils and stimulated endothelial cells.</jats:p> |
| doi_str_mv | 10.1083/jcb.111.6.3129 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTExLjYuMzEyOQ |
| imprint | Rockefeller University Press, 1990 |
| imprint_str_mv | Rockefeller University Press, 1990 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 0021-9525, 1540-8140 |
| issn_str_mv | 0021-9525, 1540-8140 |
| language | English |
| last_indexed | 2024-03-01T17:45:52.106Z |
| match_str | diamond1990icam1cd54acounterreceptorformac1cd11bcd18 |
| mega_collection | Rockefeller University Press (CrossRef) |
| physical | 3129-3139 |
| publishDate | 1990 |
| publishDateSort | 1990 |
| publisher | Rockefeller University Press |
| record_format | ai |
| recordtype | ai |
| series | The Journal of cell biology |
| source_id | 49 |
| spelling | Diamond, M S Staunton, D E de Fougerolles, A R Stacker, S A Garcia-Aguilar, J Hibbs, M L Springer, T A 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.111.6.3129 <jats:p>While the leukocyte integrin lymphocyte function-associated antigen (LFA)-1 has been demonstrated to bind intercellular adhesion molecule (ICAM)-1, results with the related Mac-1 molecule have been controversial. We have used multiple cell binding assays, purified Mac-1 and ICAM-1, and cell lines transfected with Mac-1 and ICAM-1 cDNAs to examine the interaction of ICAM-1 with Mac-1. Stimulated human umbilical vein endothelial cells (HUVECs), which express a high surface density of ICAM-1, bind to immunoaffinity-purified Mac-1 adsorbed to artificial substrates in a manner that is inhibited by mAbs to Mac-1 and ICAM-1. Transfected murine L cells or monkey COS cells expressing human ICAM-1 bind to purified Mac-1 in a specific and dose-dependent manner; the attachment to Mac-1 is more temperature sensitive, lower in avidity, and blocked by a different series of ICAM-1 mAbs when compared to LFA-1. In a reciprocal assay, COS cells cotransfected with the alpha and beta chain cDNAs of Mac-1 or LFA-1 attach to immunoaffinity-purified ICAM-1 substrates; this adhesion is blocked by mAbs to ICAM-1 and Mac-1 or LFA-1. Two color fluorescence cell conjugate experiments show that neutrophils stimulated with fMLP bind to HUVEC stimulated with lipopolysaccharide for 24 h in an ICAM-1-, Mac-1-, and LFA-1-dependent fashion. Because cellular and purified Mac-1 interact with cellular and purified ICAM-1, we conclude that ICAM-1 is a counter receptor for Mac-1 and that this receptor pair is responsible, in part, for the adhesion between stimulated neutrophils and stimulated endothelial cells.</jats:p> ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). The Journal of cell biology |
| spellingShingle | Diamond, M S, Staunton, D E, de Fougerolles, A R, Stacker, S A, Garcia-Aguilar, J, Hibbs, M L, Springer, T A, The Journal of cell biology, ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18)., Cell Biology |
| title | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_full | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_fullStr | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_full_unstemmed | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_short | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| title_sort | icam-1 (cd54): a counter-receptor for mac-1 (cd11b/cd18). |
| title_unstemmed | ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18). |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.111.6.3129 |