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Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate.
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| Zeitschriftentitel: | The Journal of cell biology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | The Journal of cell biology, 101, 1985, 1, S. 53-59 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Zanetti, M Ratcliffe, A Watt, F M Zanetti, M Ratcliffe, A Watt, F M |
|---|---|
| author |
Zanetti, M Ratcliffe, A Watt, F M |
| spellingShingle |
Zanetti, M Ratcliffe, A Watt, F M The Journal of cell biology Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. Cell Biology |
| author_sort |
zanetti, m |
| spelling |
Zanetti, M Ratcliffe, A Watt, F M 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.101.1.53 <jats:p>We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.</jats:p> Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. The Journal of cell biology |
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1985 |
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The Journal of cell biology |
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| title |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_unstemmed |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_full |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_fullStr |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_full_unstemmed |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_short |
Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_sort |
two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.101.1.53 |
| publishDate |
1985 |
| physical |
53-59 |
| description |
<jats:p>We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.</jats:p> |
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| description | <jats:p>We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.</jats:p> |
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| imprint | Rockefeller University Press, 1985 |
| imprint_str_mv | Rockefeller University Press, 1985 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| physical | 53-59 |
| publishDate | 1985 |
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| publisher | Rockefeller University Press |
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| series | The Journal of cell biology |
| source_id | 49 |
| spelling | Zanetti, M Ratcliffe, A Watt, F M 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.101.1.53 <jats:p>We have prepared a monoclonal antibody, named MZ15, that specifically binds keratan sulfate. Immunofluorescence studies showed that the distribution of keratan sulfate in articular cartilage was not uniform: the amount of keratan sulfate increased with distance from the articular surface. Two subpopulations of chondrocytes could be distinguished after isolation from cartilage by the presence or absence of cell surface keratan sulfate. Keratan sulfate-negative chondrocytes were shown to come from the upper cartilage layers. There was therefore a direct correlation between biochemical heterogeneity of cartilage matrix and heterogeneity within the chondrocyte population. During growth in monolayer culture, superficial chondrocytes began to synthesize keratan sulfate, but the cells could still be distinguished from cultures of deep or unfractionated chondrocytes by their reduced substrate adhesiveness and tendency to remain rounded.</jats:p> Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. The Journal of cell biology |
| spellingShingle | Zanetti, M, Ratcliffe, A, Watt, F M, The Journal of cell biology, Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate., Cell Biology |
| title | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_full | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_fullStr | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_full_unstemmed | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_short | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_sort | two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| title_unstemmed | Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.101.1.53 |