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Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells
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| Zeitschriftentitel: | IUBMB Life |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | IUBMB Life, 41, 1997, 2, S. 285-292 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki |
|---|---|
| author |
Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki |
| spellingShingle |
Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki IUBMB Life Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells Cell Biology Clinical Biochemistry Genetics Molecular Biology Biochemistry |
| author_sort |
lee, kwang youl |
| spelling |
Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki 1521-6543 1521-6551 Wiley Cell Biology Clinical Biochemistry Genetics Molecular Biology Biochemistry http://dx.doi.org/10.1080/15216549700201291 <jats:title>Abstract</jats:title><jats:p>In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK‐HEP‐1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 μM aloesin. The aloesin‐induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells. In addition, immuno‐complex kinase assays showed that aloesin up‐regulated the enzyme activity of cyclin E/CDK2 kinase in a dose‐dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK‐HEP‐1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up‐regulation of cyclin E‐dependent kinase activity.</jats:p> Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells IUBMB Life |
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| title |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_unstemmed |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_full |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_fullStr |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_full_unstemmed |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_short |
Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_sort |
aloesin up‐regulates cyclin e/cdk2 kinase activity via inducing the protein levels of cyclin e, cdk2, and cdc25a in sk‐hep‐1 cells |
| topic |
Cell Biology Clinical Biochemistry Genetics Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1080/15216549700201291 |
| publishDate |
1997 |
| physical |
285-292 |
| description |
<jats:title>Abstract</jats:title><jats:p>In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK‐HEP‐1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 μM aloesin. The aloesin‐induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells. In addition, immuno‐complex kinase assays showed that aloesin up‐regulated the enzyme activity of cyclin E/CDK2 kinase in a dose‐dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK‐HEP‐1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up‐regulation of cyclin E‐dependent kinase activity.</jats:p> |
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| author | Lee, Kwang Youl, Park, Jeong Hill, Chung, Myung Hee, Park, Young In, Kim, Kyu Won, Lee, Young Joo, Lee, Seung Ki |
| author_facet | Lee, Kwang Youl, Park, Jeong Hill, Chung, Myung Hee, Park, Young In, Kim, Kyu Won, Lee, Young Joo, Lee, Seung Ki, Lee, Kwang Youl, Park, Jeong Hill, Chung, Myung Hee, Park, Young In, Kim, Kyu Won, Lee, Young Joo, Lee, Seung Ki |
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| description | <jats:title>Abstract</jats:title><jats:p>In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK‐HEP‐1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 μM aloesin. The aloesin‐induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells. In addition, immuno‐complex kinase assays showed that aloesin up‐regulated the enzyme activity of cyclin E/CDK2 kinase in a dose‐dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK‐HEP‐1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up‐regulation of cyclin E‐dependent kinase activity.</jats:p> |
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| imprint | Wiley, 1997 |
| imprint_str_mv | Wiley, 1997 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 285-292 |
| publishDate | 1997 |
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| spelling | Lee, Kwang Youl Park, Jeong Hill Chung, Myung Hee Park, Young In Kim, Kyu Won Lee, Young Joo Lee, Seung Ki 1521-6543 1521-6551 Wiley Cell Biology Clinical Biochemistry Genetics Molecular Biology Biochemistry http://dx.doi.org/10.1080/15216549700201291 <jats:title>Abstract</jats:title><jats:p>In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK‐HEP‐1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 μM aloesin. The aloesin‐induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells. In addition, immuno‐complex kinase assays showed that aloesin up‐regulated the enzyme activity of cyclin E/CDK2 kinase in a dose‐dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK‐HEP‐1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up‐regulation of cyclin E‐dependent kinase activity.</jats:p> Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells IUBMB Life |
| spellingShingle | Lee, Kwang Youl, Park, Jeong Hill, Chung, Myung Hee, Park, Young In, Kim, Kyu Won, Lee, Young Joo, Lee, Seung Ki, IUBMB Life, Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells, Cell Biology, Clinical Biochemistry, Genetics, Molecular Biology, Biochemistry |
| title | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_full | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_fullStr | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_full_unstemmed | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_short | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| title_sort | aloesin up‐regulates cyclin e/cdk2 kinase activity via inducing the protein levels of cyclin e, cdk2, and cdc25a in sk‐hep‐1 cells |
| title_unstemmed | Aloesin up‐regulates cyclin E/CDK2 kinase activity via inducing the protein levels of cyclin E, CDK2, and CDC25A in SK‐HEP‐1 cells |
| topic | Cell Biology, Clinical Biochemistry, Genetics, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1080/15216549700201291 |