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Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report
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| Zeitschriftentitel: | Toxicologic Pathology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Toxicologic Pathology, 31, 2003, 1, S. 60-79 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
SAGE Publications
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| Schlagwörter: |
| author_facet |
Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette |
|---|---|
| author |
Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette |
| spellingShingle |
Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette Toxicologic Pathology Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report Cell Biology Toxicology Molecular Biology Pathology and Forensic Medicine |
| author_sort |
ittrich, carina |
| spelling |
Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette 0192-6233 1533-1601 SAGE Publications Cell Biology Toxicology Molecular Biology Pathology and Forensic Medicine http://dx.doi.org/10.1080/01926230390173888 <jats:p> A rat liver foci bioassay (RLFB) based on an initiation-promotion protocol employing preneoplastic foci of altered hepatocytes (FAH) as an endpoint, was prevalidated in 5 different laboratories. FAH were identified by immunohistochemical demonstration of glutathione- S-transferase (placental form, GSTP) and by staining with hematoxilin/eosin (H&E), and their area fraction was quantified morphometrically. The four model hepatocarcinogens N-nitrosomorpholine, 2-acetylaminofluoren, phenobarbital, and clofibrate were selected according to characteristic differences in their presumed mode of action, and tested in a total of 1,600 male and female rats at 2 different dose levels. The chemicals were found to differ characteristically in their potency and dose-response relationship to induce FAH when given alone or when administered following initiation with diethylnitrosamine. The interlaboratory variation was small for results obtained with the GSTP-stain and somewhat larger with respect to H&E. The assessment of the carcinogenic potential of the four chemicals by the different laboratories was in the same range and the nature of their dose-response relationships did not differ essentially between laboratories. Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action. </jats:p> Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report Toxicologic Pathology |
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| title |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_unstemmed |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_full |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_fullStr |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_full_unstemmed |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_short |
Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_sort |
prevalidation of a rat liver foci bioassay (rlfb) based on results from 1600 rats: a study report |
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Cell Biology Toxicology Molecular Biology Pathology and Forensic Medicine |
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http://dx.doi.org/10.1080/01926230390173888 |
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2003 |
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60-79 |
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<jats:p> A rat liver foci bioassay (RLFB) based on an initiation-promotion protocol employing preneoplastic foci of altered hepatocytes (FAH) as an endpoint, was prevalidated in 5 different laboratories. FAH were identified by immunohistochemical demonstration of glutathione- S-transferase (placental form, GSTP) and by staining with hematoxilin/eosin (H&E), and their area fraction was quantified morphometrically. The four model hepatocarcinogens N-nitrosomorpholine, 2-acetylaminofluoren, phenobarbital, and clofibrate were selected according to characteristic differences in their presumed mode of action, and tested in a total of 1,600 male and female rats at 2 different dose levels. The chemicals were found to differ characteristically in their potency and dose-response relationship to induce FAH when given alone or when administered following initiation with diethylnitrosamine. The interlaboratory variation was small for results obtained with the GSTP-stain and somewhat larger with respect to H&E. The assessment of the carcinogenic potential of the four chemicals by the different laboratories was in the same range and the nature of their dose-response relationships did not differ essentially between laboratories. Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action. </jats:p> |
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| description | <jats:p> A rat liver foci bioassay (RLFB) based on an initiation-promotion protocol employing preneoplastic foci of altered hepatocytes (FAH) as an endpoint, was prevalidated in 5 different laboratories. FAH were identified by immunohistochemical demonstration of glutathione- S-transferase (placental form, GSTP) and by staining with hematoxilin/eosin (H&E), and their area fraction was quantified morphometrically. The four model hepatocarcinogens N-nitrosomorpholine, 2-acetylaminofluoren, phenobarbital, and clofibrate were selected according to characteristic differences in their presumed mode of action, and tested in a total of 1,600 male and female rats at 2 different dose levels. The chemicals were found to differ characteristically in their potency and dose-response relationship to induce FAH when given alone or when administered following initiation with diethylnitrosamine. The interlaboratory variation was small for results obtained with the GSTP-stain and somewhat larger with respect to H&E. The assessment of the carcinogenic potential of the four chemicals by the different laboratories was in the same range and the nature of their dose-response relationships did not differ essentially between laboratories. Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action. </jats:p> |
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| spelling | Ittrich, Carina Deml, Erhard Oesterle, Doris Küttler, Karin Mellert, Werner Brendler-Schwaab, Susanne Enzmann, Harald Schladt, Ludwig Bannasch, Peter Haertel, Thomas Mönnikes, Oliver Schwarz, Michael Kopp-Schneider, Annette 0192-6233 1533-1601 SAGE Publications Cell Biology Toxicology Molecular Biology Pathology and Forensic Medicine http://dx.doi.org/10.1080/01926230390173888 <jats:p> A rat liver foci bioassay (RLFB) based on an initiation-promotion protocol employing preneoplastic foci of altered hepatocytes (FAH) as an endpoint, was prevalidated in 5 different laboratories. FAH were identified by immunohistochemical demonstration of glutathione- S-transferase (placental form, GSTP) and by staining with hematoxilin/eosin (H&E), and their area fraction was quantified morphometrically. The four model hepatocarcinogens N-nitrosomorpholine, 2-acetylaminofluoren, phenobarbital, and clofibrate were selected according to characteristic differences in their presumed mode of action, and tested in a total of 1,600 male and female rats at 2 different dose levels. The chemicals were found to differ characteristically in their potency and dose-response relationship to induce FAH when given alone or when administered following initiation with diethylnitrosamine. The interlaboratory variation was small for results obtained with the GSTP-stain and somewhat larger with respect to H&E. The assessment of the carcinogenic potential of the four chemicals by the different laboratories was in the same range and the nature of their dose-response relationships did not differ essentially between laboratories. Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action. </jats:p> Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report Toxicologic Pathology |
| spellingShingle | Ittrich, Carina, Deml, Erhard, Oesterle, Doris, Küttler, Karin, Mellert, Werner, Brendler-Schwaab, Susanne, Enzmann, Harald, Schladt, Ludwig, Bannasch, Peter, Haertel, Thomas, Mönnikes, Oliver, Schwarz, Michael, Kopp-Schneider, Annette, Toxicologic Pathology, Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report, Cell Biology, Toxicology, Molecular Biology, Pathology and Forensic Medicine |
| title | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_full | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_fullStr | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_full_unstemmed | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_short | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| title_sort | prevalidation of a rat liver foci bioassay (rlfb) based on results from 1600 rats: a study report |
| title_unstemmed | Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report |
| topic | Cell Biology, Toxicology, Molecular Biology, Pathology and Forensic Medicine |
| url | http://dx.doi.org/10.1080/01926230390173888 |