Eintrag weiter verarbeiten
On the role of macrophages in anthrax.
Gespeichert in:
| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 90, 1993, 21, S. 10198-10201 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Hanna, P C Acosta, D Collier, R J Hanna, P C Acosta, D Collier, R J |
|---|---|
| author |
Hanna, P C Acosta, D Collier, R J |
| spellingShingle |
Hanna, P C Acosta, D Collier, R J Proceedings of the National Academy of Sciences On the role of macrophages in anthrax. Multidisciplinary |
| author_sort |
hanna, p c |
| spelling |
Hanna, P C Acosta, D Collier, R J 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.90.21.10198 <jats:p>Bacillus anthracis, the causative agent of anthrax, produces systemic shock and death in susceptible animals, primarily through the action of its lethal toxin. This toxin, at high concentrations, induces lysis of macrophages in vitro but shows little or no effect on other cells. We found that when mice were specifically depleted of macrophages by silica injections, they became resistant to the toxin. Sensitivity could be restored by coinjection of toxin-sensitive cultured macrophages (RAW 264.7 cells) but not by coinjection of other cell lines tested. These results implied that macrophages mediate the action of lethal toxin in vivo and led us to investigate their role in death of the mammalian host. Sublytic concentrations of lethal toxin, orders of magnitude lower than those required to induce lysis of RAW 264.7 cells, were found to induce these cells to express interleukin 1 (IL-1) and tumor necrosis factor in vitro. Passive immunization against IL-1 or injection of an IL-1 receptor antagonist protected mice from toxin challenge, whereas anti-tumor necrosis factor provided little, if any, protection. These results imply that systemic shock and death from anthrax result primarily from the effects of high levels of cytokines, principally IL-1, produced by macrophages that have been stimulated by the anthrax lethal toxin.</jats:p> On the role of macrophages in anthrax. Proceedings of the National Academy of Sciences |
| doi_str_mv |
10.1073/pnas.90.21.10198 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjkwLjIxLjEwMTk4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjkwLjIxLjEwMTk4 |
| institution |
DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Proceedings of the National Academy of Sciences, 1993 |
| imprint_str_mv |
Proceedings of the National Academy of Sciences, 1993 |
| issn |
0027-8424 1091-6490 |
| issn_str_mv |
0027-8424 1091-6490 |
| language |
English |
| mega_collection |
Proceedings of the National Academy of Sciences (CrossRef) |
| match_str |
hanna1993ontheroleofmacrophagesinanthrax |
| publishDateSort |
1993 |
| publisher |
Proceedings of the National Academy of Sciences |
| recordtype |
ai |
| record_format |
ai |
| series |
Proceedings of the National Academy of Sciences |
| source_id |
49 |
| title |
On the role of macrophages in anthrax. |
| title_unstemmed |
On the role of macrophages in anthrax. |
| title_full |
On the role of macrophages in anthrax. |
| title_fullStr |
On the role of macrophages in anthrax. |
| title_full_unstemmed |
On the role of macrophages in anthrax. |
| title_short |
On the role of macrophages in anthrax. |
| title_sort |
on the role of macrophages in anthrax. |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.90.21.10198 |
| publishDate |
1993 |
| physical |
10198-10201 |
| description |
<jats:p>Bacillus anthracis, the causative agent of anthrax, produces systemic shock and death in susceptible animals, primarily through the action of its lethal toxin. This toxin, at high concentrations, induces lysis of macrophages in vitro but shows little or no effect on other cells. We found that when mice were specifically depleted of macrophages by silica injections, they became resistant to the toxin. Sensitivity could be restored by coinjection of toxin-sensitive cultured macrophages (RAW 264.7 cells) but not by coinjection of other cell lines tested. These results implied that macrophages mediate the action of lethal toxin in vivo and led us to investigate their role in death of the mammalian host. Sublytic concentrations of lethal toxin, orders of magnitude lower than those required to induce lysis of RAW 264.7 cells, were found to induce these cells to express interleukin 1 (IL-1) and tumor necrosis factor in vitro. Passive immunization against IL-1 or injection of an IL-1 receptor antagonist protected mice from toxin challenge, whereas anti-tumor necrosis factor provided little, if any, protection. These results imply that systemic shock and death from anthrax result primarily from the effects of high levels of cytokines, principally IL-1, produced by macrophages that have been stimulated by the anthrax lethal toxin.</jats:p> |
| container_issue |
21 |
| container_start_page |
10198 |
| container_title |
Proceedings of the National Academy of Sciences |
| container_volume |
90 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792348004478353417 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:03:58.606Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=On+the+role+of+macrophages+in+anthrax.&rft.date=1993-11-01&genre=article&issn=1091-6490&volume=90&issue=21&spage=10198&epage=10201&pages=10198-10201&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=On+the+role+of+macrophages+in+anthrax.&aulast=Collier&aufirst=R+J&rft_id=info%3Adoi%2F10.1073%2Fpnas.90.21.10198&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792348004478353417 |
| author | Hanna, P C, Acosta, D, Collier, R J |
| author_facet | Hanna, P C, Acosta, D, Collier, R J, Hanna, P C, Acosta, D, Collier, R J |
| author_sort | hanna, p c |
| container_issue | 21 |
| container_start_page | 10198 |
| container_title | Proceedings of the National Academy of Sciences |
| container_volume | 90 |
| description | <jats:p>Bacillus anthracis, the causative agent of anthrax, produces systemic shock and death in susceptible animals, primarily through the action of its lethal toxin. This toxin, at high concentrations, induces lysis of macrophages in vitro but shows little or no effect on other cells. We found that when mice were specifically depleted of macrophages by silica injections, they became resistant to the toxin. Sensitivity could be restored by coinjection of toxin-sensitive cultured macrophages (RAW 264.7 cells) but not by coinjection of other cell lines tested. These results implied that macrophages mediate the action of lethal toxin in vivo and led us to investigate their role in death of the mammalian host. Sublytic concentrations of lethal toxin, orders of magnitude lower than those required to induce lysis of RAW 264.7 cells, were found to induce these cells to express interleukin 1 (IL-1) and tumor necrosis factor in vitro. Passive immunization against IL-1 or injection of an IL-1 receptor antagonist protected mice from toxin challenge, whereas anti-tumor necrosis factor provided little, if any, protection. These results imply that systemic shock and death from anthrax result primarily from the effects of high levels of cytokines, principally IL-1, produced by macrophages that have been stimulated by the anthrax lethal toxin.</jats:p> |
| doi_str_mv | 10.1073/pnas.90.21.10198 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjkwLjIxLjEwMTk4 |
| imprint | Proceedings of the National Academy of Sciences, 1993 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1993 |
| institution | DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0027-8424, 1091-6490 |
| issn_str_mv | 0027-8424, 1091-6490 |
| language | English |
| last_indexed | 2024-03-01T18:03:58.606Z |
| match_str | hanna1993ontheroleofmacrophagesinanthrax |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 10198-10201 |
| publishDate | 1993 |
| publishDateSort | 1993 |
| publisher | Proceedings of the National Academy of Sciences |
| record_format | ai |
| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
| source_id | 49 |
| spelling | Hanna, P C Acosta, D Collier, R J 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.90.21.10198 <jats:p>Bacillus anthracis, the causative agent of anthrax, produces systemic shock and death in susceptible animals, primarily through the action of its lethal toxin. This toxin, at high concentrations, induces lysis of macrophages in vitro but shows little or no effect on other cells. We found that when mice were specifically depleted of macrophages by silica injections, they became resistant to the toxin. Sensitivity could be restored by coinjection of toxin-sensitive cultured macrophages (RAW 264.7 cells) but not by coinjection of other cell lines tested. These results implied that macrophages mediate the action of lethal toxin in vivo and led us to investigate their role in death of the mammalian host. Sublytic concentrations of lethal toxin, orders of magnitude lower than those required to induce lysis of RAW 264.7 cells, were found to induce these cells to express interleukin 1 (IL-1) and tumor necrosis factor in vitro. Passive immunization against IL-1 or injection of an IL-1 receptor antagonist protected mice from toxin challenge, whereas anti-tumor necrosis factor provided little, if any, protection. These results imply that systemic shock and death from anthrax result primarily from the effects of high levels of cytokines, principally IL-1, produced by macrophages that have been stimulated by the anthrax lethal toxin.</jats:p> On the role of macrophages in anthrax. Proceedings of the National Academy of Sciences |
| spellingShingle | Hanna, P C, Acosta, D, Collier, R J, Proceedings of the National Academy of Sciences, On the role of macrophages in anthrax., Multidisciplinary |
| title | On the role of macrophages in anthrax. |
| title_full | On the role of macrophages in anthrax. |
| title_fullStr | On the role of macrophages in anthrax. |
| title_full_unstemmed | On the role of macrophages in anthrax. |
| title_short | On the role of macrophages in anthrax. |
| title_sort | on the role of macrophages in anthrax. |
| title_unstemmed | On the role of macrophages in anthrax. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.90.21.10198 |