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Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , |
| In: | Proceedings of the National Academy of Sciences, 96, 1999, 26, S. 15044-15049 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Ray, Alo Runge, Kurt W. Ray, Alo Runge, Kurt W. |
|---|---|
| author |
Ray, Alo Runge, Kurt W. |
| spellingShingle |
Ray, Alo Runge, Kurt W. Proceedings of the National Academy of Sciences Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells Multidisciplinary |
| author_sort |
ray, alo |
| spelling |
Ray, Alo Runge, Kurt W. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.96.26.15044 <jats:p> Yeast telomere DNA consists of a continuous, ≈330-bp tract of the heterogeneous repeat TG <jats:sub>1-3</jats:sub> with irregularly spaced, high affinity sites for the protein Rap1p. Yeast monitor, or count, the number of telomeric Rap1p C termini in a negative feedback mechanism to modulate the length of the terminal TG <jats:sub>1-3</jats:sub> repeats, and synthetic telomeres that tether Rap1p molecules adjacent to the TG <jats:sub>1-3</jats:sub> tract cause wild-type cells to maintain a shorter TG <jats:sub>1-3</jats:sub> tract. To identify trans-acting proteins required to count Rap1p molecules, these same synthetic telomeres were placed in two short telomere mutants: <jats:italic>yku70Δ</jats:italic> (which lack the yeast Ku70 protein) and <jats:italic>tel1Δ</jats:italic> (which lack the yeast ortholog of ATM). Although both mutants maintain telomeres with ≈100 bp of TG <jats:sub>1-3</jats:sub> , only <jats:italic>yku70Δ</jats:italic> cells maintained shorter TG <jats:sub>1-3</jats:sub> repeats in response to internal Rap1p molecules. This distinct response to internal Rap1p molecules was not caused by a variation in Rap1p site density in the TG <jats:sub>1-3</jats:sub> repeats as sequencing of <jats:italic>tel1Δ</jats:italic> and <jats:italic>yku70Δ</jats:italic> telomeres showed that both strains have only five to six Rap1p sites per 100-bp telomere. In addition, the <jats:italic>tel1Δ</jats:italic> short telomere phenotype was epistatic to the unregulated telomere length caused by deletion of the Rap1p C-terminal domain. Thus, the length of the TG <jats:sub>1-3</jats:sub> repeats in <jats:italic>tel1Δ</jats:italic> cells was independent of the number of the Rap1p C termini at the telomere. These data indicate that <jats:italic>tel1Δ</jats:italic> cells use an alternative mechanism to regulate telomere length that is distinct from monitoring the number of telomere binding proteins. </jats:p> Varying the number of telomere-bound proteins does not alter telomere length in <i>tel1Δ</i> cells Proceedings of the National Academy of Sciences |
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Proceedings of the National Academy of Sciences, 1999 |
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ray1999varyingthenumberoftelomereboundproteinsdoesnotaltertelomerelengthintel1dcells |
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1999 |
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| title |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_unstemmed |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_full |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_fullStr |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_full_unstemmed |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_short |
Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_sort |
varying the number of telomere-bound proteins does not alter telomere length in
<i>tel1δ</i>
cells |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.96.26.15044 |
| publishDate |
1999 |
| physical |
15044-15049 |
| description |
<jats:p>
Yeast telomere DNA consists of a continuous, ≈330-bp tract of the heterogeneous repeat TG
<jats:sub>1-3</jats:sub>
with irregularly spaced, high affinity sites for the protein Rap1p. Yeast monitor, or count, the number of telomeric Rap1p C termini in a negative feedback mechanism to modulate the length of the terminal TG
<jats:sub>1-3</jats:sub>
repeats, and synthetic telomeres that tether Rap1p molecules adjacent to the TG
<jats:sub>1-3</jats:sub>
tract cause wild-type cells to maintain a shorter TG
<jats:sub>1-3</jats:sub>
tract. To identify trans-acting proteins required to count Rap1p molecules, these same synthetic telomeres were placed in two short telomere mutants:
<jats:italic>yku70Δ</jats:italic>
(which lack the yeast Ku70 protein) and
<jats:italic>tel1Δ</jats:italic>
(which lack the yeast ortholog of ATM). Although both mutants maintain telomeres with ≈100 bp of TG
<jats:sub>1-3</jats:sub>
, only
<jats:italic>yku70Δ</jats:italic>
cells maintained shorter TG
<jats:sub>1-3</jats:sub>
repeats in response to internal Rap1p molecules. This distinct response to internal Rap1p molecules was not caused by a variation in Rap1p site density in the TG
<jats:sub>1-3</jats:sub>
repeats as sequencing of
<jats:italic>tel1Δ</jats:italic>
and
<jats:italic>yku70Δ</jats:italic>
telomeres showed that both strains have only five to six Rap1p sites per 100-bp telomere. In addition, the
<jats:italic>tel1Δ</jats:italic>
short telomere phenotype was epistatic to the unregulated telomere length caused by deletion of the Rap1p C-terminal domain. Thus, the length of the TG
<jats:sub>1-3</jats:sub>
repeats in
<jats:italic>tel1Δ</jats:italic>
cells was independent of the number of the Rap1p C termini at the telomere. These data indicate that
<jats:italic>tel1Δ</jats:italic>
cells use an alternative mechanism to regulate telomere length that is distinct from monitoring the number of telomere binding proteins.
</jats:p> |
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| description | <jats:p> Yeast telomere DNA consists of a continuous, ≈330-bp tract of the heterogeneous repeat TG <jats:sub>1-3</jats:sub> with irregularly spaced, high affinity sites for the protein Rap1p. Yeast monitor, or count, the number of telomeric Rap1p C termini in a negative feedback mechanism to modulate the length of the terminal TG <jats:sub>1-3</jats:sub> repeats, and synthetic telomeres that tether Rap1p molecules adjacent to the TG <jats:sub>1-3</jats:sub> tract cause wild-type cells to maintain a shorter TG <jats:sub>1-3</jats:sub> tract. To identify trans-acting proteins required to count Rap1p molecules, these same synthetic telomeres were placed in two short telomere mutants: <jats:italic>yku70Δ</jats:italic> (which lack the yeast Ku70 protein) and <jats:italic>tel1Δ</jats:italic> (which lack the yeast ortholog of ATM). Although both mutants maintain telomeres with ≈100 bp of TG <jats:sub>1-3</jats:sub> , only <jats:italic>yku70Δ</jats:italic> cells maintained shorter TG <jats:sub>1-3</jats:sub> repeats in response to internal Rap1p molecules. This distinct response to internal Rap1p molecules was not caused by a variation in Rap1p site density in the TG <jats:sub>1-3</jats:sub> repeats as sequencing of <jats:italic>tel1Δ</jats:italic> and <jats:italic>yku70Δ</jats:italic> telomeres showed that both strains have only five to six Rap1p sites per 100-bp telomere. In addition, the <jats:italic>tel1Δ</jats:italic> short telomere phenotype was epistatic to the unregulated telomere length caused by deletion of the Rap1p C-terminal domain. Thus, the length of the TG <jats:sub>1-3</jats:sub> repeats in <jats:italic>tel1Δ</jats:italic> cells was independent of the number of the Rap1p C termini at the telomere. These data indicate that <jats:italic>tel1Δ</jats:italic> cells use an alternative mechanism to regulate telomere length that is distinct from monitoring the number of telomere binding proteins. </jats:p> |
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| spelling | Ray, Alo Runge, Kurt W. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.96.26.15044 <jats:p> Yeast telomere DNA consists of a continuous, ≈330-bp tract of the heterogeneous repeat TG <jats:sub>1-3</jats:sub> with irregularly spaced, high affinity sites for the protein Rap1p. Yeast monitor, or count, the number of telomeric Rap1p C termini in a negative feedback mechanism to modulate the length of the terminal TG <jats:sub>1-3</jats:sub> repeats, and synthetic telomeres that tether Rap1p molecules adjacent to the TG <jats:sub>1-3</jats:sub> tract cause wild-type cells to maintain a shorter TG <jats:sub>1-3</jats:sub> tract. To identify trans-acting proteins required to count Rap1p molecules, these same synthetic telomeres were placed in two short telomere mutants: <jats:italic>yku70Δ</jats:italic> (which lack the yeast Ku70 protein) and <jats:italic>tel1Δ</jats:italic> (which lack the yeast ortholog of ATM). Although both mutants maintain telomeres with ≈100 bp of TG <jats:sub>1-3</jats:sub> , only <jats:italic>yku70Δ</jats:italic> cells maintained shorter TG <jats:sub>1-3</jats:sub> repeats in response to internal Rap1p molecules. This distinct response to internal Rap1p molecules was not caused by a variation in Rap1p site density in the TG <jats:sub>1-3</jats:sub> repeats as sequencing of <jats:italic>tel1Δ</jats:italic> and <jats:italic>yku70Δ</jats:italic> telomeres showed that both strains have only five to six Rap1p sites per 100-bp telomere. In addition, the <jats:italic>tel1Δ</jats:italic> short telomere phenotype was epistatic to the unregulated telomere length caused by deletion of the Rap1p C-terminal domain. Thus, the length of the TG <jats:sub>1-3</jats:sub> repeats in <jats:italic>tel1Δ</jats:italic> cells was independent of the number of the Rap1p C termini at the telomere. These data indicate that <jats:italic>tel1Δ</jats:italic> cells use an alternative mechanism to regulate telomere length that is distinct from monitoring the number of telomere binding proteins. </jats:p> Varying the number of telomere-bound proteins does not alter telomere length in <i>tel1Δ</i> cells Proceedings of the National Academy of Sciences |
| spellingShingle | Ray, Alo, Runge, Kurt W., Proceedings of the National Academy of Sciences, Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells, Multidisciplinary |
| title | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_full | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_fullStr | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_full_unstemmed | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_short | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| title_sort | varying the number of telomere-bound proteins does not alter telomere length in <i>tel1δ</i> cells |
| title_unstemmed | Varying the number of telomere-bound proteins does not alter telomere length in tel1Δ cells |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.96.26.15044 |