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The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
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| Personen und Körperschaften: | , , , , , , , , , |
| In: | Proceedings of the National Academy of Sciences, 95, 1998, 24, S. 14435-14440 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert |
|---|---|
| author |
Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert |
| spellingShingle |
Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert Proceedings of the National Academy of Sciences The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein Multidisciplinary |
| author_sort |
puri, anu |
| spelling |
Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.95.24.14435 <jats:p>Previously, we showed that the addition of human erythrocyte glycosphingolipids (GSLs) to nonhuman CD4<jats:sup>+</jats:sup>or GSL-depleted human CD4<jats:sup>+</jats:sup>cells rendered those cells susceptible to HIV-1 envelope glycoprotein-mediated cell fusion. Individual components in the GSL mixture were isolated by fractionation on a silica-gel column and incorporated into the membranes of CD4<jats:sup>+</jats:sup>cells. GSL-supplemented target cells were then examined for their ability to fuse with TF228 cells expressing HIV-1<jats:sub>LAI</jats:sub>envelope glycoprotein. We found that one GSL fraction, fraction 3, exhibited the highest recovery of fusion after incorporation into CD4<jats:sup>+</jats:sup>nonhuman and GSL-depleted HeLa-CD4 cells and that fraction 3 contained a single GSL fraction. Fraction 3 was characterized by MS, NMR spectroscopy, enzymatic analysis, and immunostaining with an antiglobotriaosylceramide (Gb3) antibody and was found to be Gal(α1→4)Gal(β1→4)Glc-Cer (Gb3). The addition of fraction 3 or Gb3 to GSL-depleted HeLa-CD4 cells recovered fusion, but the addition of galactosylceramide, glucosylceramide, the monosialoganglioside, GM3, lactosylceramide, globoside, the disialoganglioside, GD3, or α-galactosidase A-digested fraction 3 had no effect. Our findings show that the neutral GSL, Gb3, is required for CD4/CXCR4-dependent HIV-1 fusion.</jats:p> The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein Proceedings of the National Academy of Sciences |
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| title |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_unstemmed |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_full |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_fullStr |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_full_unstemmed |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_short |
The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_sort |
the neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a cd4-dependent cxcr4-utilizing hiv type 1 envelope glycoprotein |
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Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.95.24.14435 |
| publishDate |
1998 |
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14435-14440 |
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<jats:p>Previously, we showed that the addition of human erythrocyte glycosphingolipids (GSLs) to nonhuman CD4<jats:sup>+</jats:sup>or GSL-depleted human CD4<jats:sup>+</jats:sup>cells rendered those cells susceptible to HIV-1 envelope glycoprotein-mediated cell fusion. Individual components in the GSL mixture were isolated by fractionation on a silica-gel column and incorporated into the membranes of CD4<jats:sup>+</jats:sup>cells. GSL-supplemented target cells were then examined for their ability to fuse with TF228 cells expressing HIV-1<jats:sub>LAI</jats:sub>envelope glycoprotein. We found that one GSL fraction, fraction 3, exhibited the highest recovery of fusion after incorporation into CD4<jats:sup>+</jats:sup>nonhuman and GSL-depleted HeLa-CD4 cells and that fraction 3 contained a single GSL fraction. Fraction 3 was characterized by MS, NMR spectroscopy, enzymatic analysis, and immunostaining with an antiglobotriaosylceramide (Gb3) antibody and was found to be Gal(α1→4)Gal(β1→4)Glc-Cer (Gb3). The addition of fraction 3 or Gb3 to GSL-depleted HeLa-CD4 cells recovered fusion, but the addition of galactosylceramide, glucosylceramide, the monosialoganglioside, GM3, lactosylceramide, globoside, the disialoganglioside, GD3, or α-galactosidase A-digested fraction 3 had no effect. Our findings show that the neutral GSL, Gb3, is required for CD4/CXCR4-dependent HIV-1 fusion.</jats:p> |
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| author | Puri, Anu, Hug, Peter, Jernigan, Kristine, Barchi, Joseph, Kim, Hee-Yong, Hamilton, Jillon, Wiels, Joëlle, Murray, Gary J., Brady, Roscoe O., Blumenthal, Robert |
| author_facet | Puri, Anu, Hug, Peter, Jernigan, Kristine, Barchi, Joseph, Kim, Hee-Yong, Hamilton, Jillon, Wiels, Joëlle, Murray, Gary J., Brady, Roscoe O., Blumenthal, Robert, Puri, Anu, Hug, Peter, Jernigan, Kristine, Barchi, Joseph, Kim, Hee-Yong, Hamilton, Jillon, Wiels, Joëlle, Murray, Gary J., Brady, Roscoe O., Blumenthal, Robert |
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| description | <jats:p>Previously, we showed that the addition of human erythrocyte glycosphingolipids (GSLs) to nonhuman CD4<jats:sup>+</jats:sup>or GSL-depleted human CD4<jats:sup>+</jats:sup>cells rendered those cells susceptible to HIV-1 envelope glycoprotein-mediated cell fusion. Individual components in the GSL mixture were isolated by fractionation on a silica-gel column and incorporated into the membranes of CD4<jats:sup>+</jats:sup>cells. GSL-supplemented target cells were then examined for their ability to fuse with TF228 cells expressing HIV-1<jats:sub>LAI</jats:sub>envelope glycoprotein. We found that one GSL fraction, fraction 3, exhibited the highest recovery of fusion after incorporation into CD4<jats:sup>+</jats:sup>nonhuman and GSL-depleted HeLa-CD4 cells and that fraction 3 contained a single GSL fraction. Fraction 3 was characterized by MS, NMR spectroscopy, enzymatic analysis, and immunostaining with an antiglobotriaosylceramide (Gb3) antibody and was found to be Gal(α1→4)Gal(β1→4)Glc-Cer (Gb3). The addition of fraction 3 or Gb3 to GSL-depleted HeLa-CD4 cells recovered fusion, but the addition of galactosylceramide, glucosylceramide, the monosialoganglioside, GM3, lactosylceramide, globoside, the disialoganglioside, GD3, or α-galactosidase A-digested fraction 3 had no effect. Our findings show that the neutral GSL, Gb3, is required for CD4/CXCR4-dependent HIV-1 fusion.</jats:p> |
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| imprint_str_mv | Proceedings of the National Academy of Sciences, 1998 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Puri, Anu Hug, Peter Jernigan, Kristine Barchi, Joseph Kim, Hee-Yong Hamilton, Jillon Wiels, Joëlle Murray, Gary J. Brady, Roscoe O. Blumenthal, Robert 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.95.24.14435 <jats:p>Previously, we showed that the addition of human erythrocyte glycosphingolipids (GSLs) to nonhuman CD4<jats:sup>+</jats:sup>or GSL-depleted human CD4<jats:sup>+</jats:sup>cells rendered those cells susceptible to HIV-1 envelope glycoprotein-mediated cell fusion. Individual components in the GSL mixture were isolated by fractionation on a silica-gel column and incorporated into the membranes of CD4<jats:sup>+</jats:sup>cells. GSL-supplemented target cells were then examined for their ability to fuse with TF228 cells expressing HIV-1<jats:sub>LAI</jats:sub>envelope glycoprotein. We found that one GSL fraction, fraction 3, exhibited the highest recovery of fusion after incorporation into CD4<jats:sup>+</jats:sup>nonhuman and GSL-depleted HeLa-CD4 cells and that fraction 3 contained a single GSL fraction. Fraction 3 was characterized by MS, NMR spectroscopy, enzymatic analysis, and immunostaining with an antiglobotriaosylceramide (Gb3) antibody and was found to be Gal(α1→4)Gal(β1→4)Glc-Cer (Gb3). The addition of fraction 3 or Gb3 to GSL-depleted HeLa-CD4 cells recovered fusion, but the addition of galactosylceramide, glucosylceramide, the monosialoganglioside, GM3, lactosylceramide, globoside, the disialoganglioside, GD3, or α-galactosidase A-digested fraction 3 had no effect. Our findings show that the neutral GSL, Gb3, is required for CD4/CXCR4-dependent HIV-1 fusion.</jats:p> The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein Proceedings of the National Academy of Sciences |
| spellingShingle | Puri, Anu, Hug, Peter, Jernigan, Kristine, Barchi, Joseph, Kim, Hee-Yong, Hamilton, Jillon, Wiels, Joëlle, Murray, Gary J., Brady, Roscoe O., Blumenthal, Robert, Proceedings of the National Academy of Sciences, The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein, Multidisciplinary |
| title | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_full | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_fullStr | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_full_unstemmed | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_short | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| title_sort | the neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a cd4-dependent cxcr4-utilizing hiv type 1 envelope glycoprotein |
| title_unstemmed | The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.95.24.14435 |