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Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Proceedings of the National Academy of Sciences, 94, 1997, 14, S. 7446-7451 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. |
|---|---|
| author |
Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. |
| spellingShingle |
Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. Proceedings of the National Academy of Sciences Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 Multidisciplinary |
| author_sort |
fox, mary e. |
| spelling |
Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.94.14.7446 <jats:p> The activity of the <jats:italic>M26</jats:italic> meiotic recombination hot spot of <jats:italic>Schizosaccharomyces pombe</jats:italic> depends on the presence of the heptamer 5′-ATGACGT-3′. Transplacement of DNA fragments containing the <jats:italic>ade6-M26</jats:italic> gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1–4 bp in the <jats:italic>ade6</jats:italic> and <jats:italic>ura4</jats:italic> genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for <jats:italic>M26</jats:italic> hot spot activity. </jats:p> Position- and orientation-independent activity of the <i>Schizosaccharomyces pombe</i> meiotic recombination hot spot <i>M26</i> Proceedings of the National Academy of Sciences |
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1997 |
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| title |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_unstemmed |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_full |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_fullStr |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_full_unstemmed |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_short |
Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_sort |
position- and orientation-independent activity of the
<i>schizosaccharomyces pombe</i>
meiotic recombination hot spot
<i>m26</i> |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.94.14.7446 |
| publishDate |
1997 |
| physical |
7446-7451 |
| description |
<jats:p>
The activity of the
<jats:italic>M26</jats:italic>
meiotic recombination hot spot of
<jats:italic>Schizosaccharomyces pombe</jats:italic>
depends on the presence of the heptamer 5′-ATGACGT-3′. Transplacement of DNA fragments containing the
<jats:italic>ade6-M26</jats:italic>
gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1–4 bp in the
<jats:italic>ade6</jats:italic>
and
<jats:italic>ura4</jats:italic>
genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for
<jats:italic>M26</jats:italic>
hot spot activity.
</jats:p> |
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| author | Fox, Mary E., Virgin, Jeffrey B., Metzger, Jens, Smith, Gerald R. |
| author_facet | Fox, Mary E., Virgin, Jeffrey B., Metzger, Jens, Smith, Gerald R., Fox, Mary E., Virgin, Jeffrey B., Metzger, Jens, Smith, Gerald R. |
| author_sort | fox, mary e. |
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| description | <jats:p> The activity of the <jats:italic>M26</jats:italic> meiotic recombination hot spot of <jats:italic>Schizosaccharomyces pombe</jats:italic> depends on the presence of the heptamer 5′-ATGACGT-3′. Transplacement of DNA fragments containing the <jats:italic>ade6-M26</jats:italic> gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1–4 bp in the <jats:italic>ade6</jats:italic> and <jats:italic>ura4</jats:italic> genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for <jats:italic>M26</jats:italic> hot spot activity. </jats:p> |
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| imprint_str_mv | Proceedings of the National Academy of Sciences, 1997 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4 |
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| spelling | Fox, Mary E. Virgin, Jeffrey B. Metzger, Jens Smith, Gerald R. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.94.14.7446 <jats:p> The activity of the <jats:italic>M26</jats:italic> meiotic recombination hot spot of <jats:italic>Schizosaccharomyces pombe</jats:italic> depends on the presence of the heptamer 5′-ATGACGT-3′. Transplacement of DNA fragments containing the <jats:italic>ade6-M26</jats:italic> gene to other chromosomal loci has previously demonstrated that the heptamer functions in some, but not all, transplacements, suggesting that hot spot activity depends on chromosomal context. In this study, hot spot activity was tested in the absence of gross DNA changes by using site-directed mutagenesis to create the heptamer sequence at novel locations in the genome. When created by mutagenesis of 1–4 bp in the <jats:italic>ade6</jats:italic> and <jats:italic>ura4</jats:italic> genes, the heptamer was active as a recombination hot spot, in an orientation-independent manner, at all locations tested. Thus, the heptamer sequence can create an active hot spot in other chromosomal contexts, provided that the gross chromosomal structure is not altered; this result is consistent with the hypothesis that a specific higher-order chromatin structure is required for <jats:italic>M26</jats:italic> hot spot activity. </jats:p> Position- and orientation-independent activity of the <i>Schizosaccharomyces pombe</i> meiotic recombination hot spot <i>M26</i> Proceedings of the National Academy of Sciences |
| spellingShingle | Fox, Mary E., Virgin, Jeffrey B., Metzger, Jens, Smith, Gerald R., Proceedings of the National Academy of Sciences, Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26, Multidisciplinary |
| title | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_full | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_fullStr | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_full_unstemmed | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_short | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| title_sort | position- and orientation-independent activity of the <i>schizosaccharomyces pombe</i> meiotic recombination hot spot <i>m26</i> |
| title_unstemmed | Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26 |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.94.14.7446 |