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Chromosomal context dependence of a eukaryotic recombinational hot spot.
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , |
| In: | Proceedings of the National Academy of Sciences, 89, 1992, 1, S. 227-231 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| author_facet |
Ponticelli, A S Smith, G R Ponticelli, A S Smith, G R |
|---|---|
| author |
Ponticelli, A S Smith, G R |
| spellingShingle |
Ponticelli, A S Smith, G R Proceedings of the National Academy of Sciences Chromosomal context dependence of a eukaryotic recombinational hot spot. Multidisciplinary |
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ponticelli, a s |
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Ponticelli, A S Smith, G R 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.89.1.227 <jats:p>The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.</jats:p> Chromosomal context dependence of a eukaryotic recombinational hot spot. Proceedings of the National Academy of Sciences |
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| title |
Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_unstemmed |
Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_full |
Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_fullStr |
Chromosomal context dependence of a eukaryotic recombinational hot spot. |
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Chromosomal context dependence of a eukaryotic recombinational hot spot. |
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Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_sort |
chromosomal context dependence of a eukaryotic recombinational hot spot. |
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Multidisciplinary |
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http://dx.doi.org/10.1073/pnas.89.1.227 |
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1992 |
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227-231 |
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<jats:p>The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.</jats:p> |
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| description | <jats:p>The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.</jats:p> |
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| imprint | Proceedings of the National Academy of Sciences, 1992 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1992 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
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| spelling | Ponticelli, A S Smith, G R 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.89.1.227 <jats:p>The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.</jats:p> Chromosomal context dependence of a eukaryotic recombinational hot spot. Proceedings of the National Academy of Sciences |
| spellingShingle | Ponticelli, A S, Smith, G R, Proceedings of the National Academy of Sciences, Chromosomal context dependence of a eukaryotic recombinational hot spot., Multidisciplinary |
| title | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_full | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_fullStr | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_full_unstemmed | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_short | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_sort | chromosomal context dependence of a eukaryotic recombinational hot spot. |
| title_unstemmed | Chromosomal context dependence of a eukaryotic recombinational hot spot. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.89.1.227 |