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Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA.
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , |
| In: | Proceedings of the National Academy of Sciences, 84, 1987, 4, S. 910-914 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Mendel, D Dervan, P B Mendel, D Dervan, P B |
|---|---|
| author |
Mendel, D Dervan, P B |
| spellingShingle |
Mendel, D Dervan, P B Proceedings of the National Academy of Sciences Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. Multidisciplinary |
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mendel, d |
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Mendel, D Dervan, P B 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.84.4.910 <jats:p>Forms of the DNA double helix containing non-Watson-Crick base-pairing have been discovered recently based on x-ray diffraction analysis of quinoxaline antibiotic-oligonucleotide complexes. In an effort to find evidence for Hoogsteen base-pairing at quinoxaline-binding sites in solution, chemical "footprinting" (differential cleavage reactivity) of echinomycin bound to DNA restriction fragments was examined. We report that purines (A greater than G) in the first and/or fourth base-pair positions of occupied echinomycin-binding sites are hyperreactive to diethyl pyrocarbonate. The correspondence of the solid-state data and the sites of diethyl pyrocarbonate hyperreactivity suggests that diethyl pyrocarbonate may be a sensitive reagent for the detection of Hoogsteen base-pairing in solution. Moreover, a 12-base-pair segment of alternating A-T DNA, which is 6 base pairs away from the nearest strong echinomycin-binding site, is also hyperreactive to diethyl pyrocarbonate in the presence of echinomycin. This hyperreactive segment may be an altered form of right-handed DNA that is entirely Hoogsteen base-paired.</jats:p> Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. Proceedings of the National Academy of Sciences |
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| title |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_unstemmed |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_full |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_fullStr |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_full_unstemmed |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_short |
Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_sort |
hoogsteen base pairs proximal and distal to echinomycin binding sites on dna. |
| topic |
Multidisciplinary |
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http://dx.doi.org/10.1073/pnas.84.4.910 |
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1987 |
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910-914 |
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<jats:p>Forms of the DNA double helix containing non-Watson-Crick base-pairing have been discovered recently based on x-ray diffraction analysis of quinoxaline antibiotic-oligonucleotide complexes. In an effort to find evidence for Hoogsteen base-pairing at quinoxaline-binding sites in solution, chemical "footprinting" (differential cleavage reactivity) of echinomycin bound to DNA restriction fragments was examined. We report that purines (A greater than G) in the first and/or fourth base-pair positions of occupied echinomycin-binding sites are hyperreactive to diethyl pyrocarbonate. The correspondence of the solid-state data and the sites of diethyl pyrocarbonate hyperreactivity suggests that diethyl pyrocarbonate may be a sensitive reagent for the detection of Hoogsteen base-pairing in solution. Moreover, a 12-base-pair segment of alternating A-T DNA, which is 6 base pairs away from the nearest strong echinomycin-binding site, is also hyperreactive to diethyl pyrocarbonate in the presence of echinomycin. This hyperreactive segment may be an altered form of right-handed DNA that is entirely Hoogsteen base-paired.</jats:p> |
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| description | <jats:p>Forms of the DNA double helix containing non-Watson-Crick base-pairing have been discovered recently based on x-ray diffraction analysis of quinoxaline antibiotic-oligonucleotide complexes. In an effort to find evidence for Hoogsteen base-pairing at quinoxaline-binding sites in solution, chemical "footprinting" (differential cleavage reactivity) of echinomycin bound to DNA restriction fragments was examined. We report that purines (A greater than G) in the first and/or fourth base-pair positions of occupied echinomycin-binding sites are hyperreactive to diethyl pyrocarbonate. The correspondence of the solid-state data and the sites of diethyl pyrocarbonate hyperreactivity suggests that diethyl pyrocarbonate may be a sensitive reagent for the detection of Hoogsteen base-pairing in solution. Moreover, a 12-base-pair segment of alternating A-T DNA, which is 6 base pairs away from the nearest strong echinomycin-binding site, is also hyperreactive to diethyl pyrocarbonate in the presence of echinomycin. This hyperreactive segment may be an altered form of right-handed DNA that is entirely Hoogsteen base-paired.</jats:p> |
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| spelling | Mendel, D Dervan, P B 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.84.4.910 <jats:p>Forms of the DNA double helix containing non-Watson-Crick base-pairing have been discovered recently based on x-ray diffraction analysis of quinoxaline antibiotic-oligonucleotide complexes. In an effort to find evidence for Hoogsteen base-pairing at quinoxaline-binding sites in solution, chemical "footprinting" (differential cleavage reactivity) of echinomycin bound to DNA restriction fragments was examined. We report that purines (A greater than G) in the first and/or fourth base-pair positions of occupied echinomycin-binding sites are hyperreactive to diethyl pyrocarbonate. The correspondence of the solid-state data and the sites of diethyl pyrocarbonate hyperreactivity suggests that diethyl pyrocarbonate may be a sensitive reagent for the detection of Hoogsteen base-pairing in solution. Moreover, a 12-base-pair segment of alternating A-T DNA, which is 6 base pairs away from the nearest strong echinomycin-binding site, is also hyperreactive to diethyl pyrocarbonate in the presence of echinomycin. This hyperreactive segment may be an altered form of right-handed DNA that is entirely Hoogsteen base-paired.</jats:p> Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. Proceedings of the National Academy of Sciences |
| spellingShingle | Mendel, D, Dervan, P B, Proceedings of the National Academy of Sciences, Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA., Multidisciplinary |
| title | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_full | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_fullStr | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_full_unstemmed | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_short | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| title_sort | hoogsteen base pairs proximal and distal to echinomycin binding sites on dna. |
| title_unstemmed | Hoogsteen base pairs proximal and distal to echinomycin binding sites on DNA. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.84.4.910 |