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Saccharin and cyclamate inhibit binding of epidermal growth factor.
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | |
| In: | Proceedings of the National Academy of Sciences, 78, 1981, 2, S. 1042-1046 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Lee, L S Lee, L S |
|---|---|
| author |
Lee, L S |
| spellingShingle |
Lee, L S Proceedings of the National Academy of Sciences Saccharin and cyclamate inhibit binding of epidermal growth factor. Multidisciplinary |
| author_sort |
lee, l s |
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Lee, L S 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.78.2.1042 <jats:p>The binding of 125I-labeled mouse epidermal growth factor (EGF) to 18 cell lines, including HeLa (human carcinoma), MDCK (dog kidney cells), HTC (rat hepatoma), K22 (rat liver), HF (human foreskin), GM17 (human skin fibroblasts), XP (human xeroderma pigmentosum fibroblasts), and 3T3-L1 (mouse fibroblasts), was inhibited by saccharin and cyclamate. The human cells were more sensitive to inhibition by these sweeteners than mouse or rat cells. EGF at doses far above the physiological levels reversed the inhibition in rodent cells but not in HeLa cells. In HeLa cells, the doses of saccharin and cyclamate needed for 50% inhibition were 3.5 and 9.3 mg/ml, respectively. Glucose, 2-deoxyglucose, sucrose, and xylitol did not inhibit EGF binding. Previous studies have shown that phorbol esters, strongly potent tumor promoters, also inhibit EGF binding to tissue culture cells. To explain the EGF binding inhibition by such greatly dissimilar molecules as phorbol esters, saccharin, and cyclamate, it is suggested that they operate through the activation of a hormone response control unit.</jats:p> Saccharin and cyclamate inhibit binding of epidermal growth factor. Proceedings of the National Academy of Sciences |
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Proceedings of the National Academy of Sciences |
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| title |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_unstemmed |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_full |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_fullStr |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_full_unstemmed |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_short |
Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_sort |
saccharin and cyclamate inhibit binding of epidermal growth factor. |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.78.2.1042 |
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1981 |
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1042-1046 |
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<jats:p>The binding of 125I-labeled mouse epidermal growth factor (EGF) to 18 cell lines, including HeLa (human carcinoma), MDCK (dog kidney cells), HTC (rat hepatoma), K22 (rat liver), HF (human foreskin), GM17 (human skin fibroblasts), XP (human xeroderma pigmentosum fibroblasts), and 3T3-L1 (mouse fibroblasts), was inhibited by saccharin and cyclamate. The human cells were more sensitive to inhibition by these sweeteners than mouse or rat cells. EGF at doses far above the physiological levels reversed the inhibition in rodent cells but not in HeLa cells. In HeLa cells, the doses of saccharin and cyclamate needed for 50% inhibition were 3.5 and 9.3 mg/ml, respectively. Glucose, 2-deoxyglucose, sucrose, and xylitol did not inhibit EGF binding. Previous studies have shown that phorbol esters, strongly potent tumor promoters, also inhibit EGF binding to tissue culture cells. To explain the EGF binding inhibition by such greatly dissimilar molecules as phorbol esters, saccharin, and cyclamate, it is suggested that they operate through the activation of a hormone response control unit.</jats:p> |
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| description | <jats:p>The binding of 125I-labeled mouse epidermal growth factor (EGF) to 18 cell lines, including HeLa (human carcinoma), MDCK (dog kidney cells), HTC (rat hepatoma), K22 (rat liver), HF (human foreskin), GM17 (human skin fibroblasts), XP (human xeroderma pigmentosum fibroblasts), and 3T3-L1 (mouse fibroblasts), was inhibited by saccharin and cyclamate. The human cells were more sensitive to inhibition by these sweeteners than mouse or rat cells. EGF at doses far above the physiological levels reversed the inhibition in rodent cells but not in HeLa cells. In HeLa cells, the doses of saccharin and cyclamate needed for 50% inhibition were 3.5 and 9.3 mg/ml, respectively. Glucose, 2-deoxyglucose, sucrose, and xylitol did not inhibit EGF binding. Previous studies have shown that phorbol esters, strongly potent tumor promoters, also inhibit EGF binding to tissue culture cells. To explain the EGF binding inhibition by such greatly dissimilar molecules as phorbol esters, saccharin, and cyclamate, it is suggested that they operate through the activation of a hormone response control unit.</jats:p> |
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| spelling | Lee, L S 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.78.2.1042 <jats:p>The binding of 125I-labeled mouse epidermal growth factor (EGF) to 18 cell lines, including HeLa (human carcinoma), MDCK (dog kidney cells), HTC (rat hepatoma), K22 (rat liver), HF (human foreskin), GM17 (human skin fibroblasts), XP (human xeroderma pigmentosum fibroblasts), and 3T3-L1 (mouse fibroblasts), was inhibited by saccharin and cyclamate. The human cells were more sensitive to inhibition by these sweeteners than mouse or rat cells. EGF at doses far above the physiological levels reversed the inhibition in rodent cells but not in HeLa cells. In HeLa cells, the doses of saccharin and cyclamate needed for 50% inhibition were 3.5 and 9.3 mg/ml, respectively. Glucose, 2-deoxyglucose, sucrose, and xylitol did not inhibit EGF binding. Previous studies have shown that phorbol esters, strongly potent tumor promoters, also inhibit EGF binding to tissue culture cells. To explain the EGF binding inhibition by such greatly dissimilar molecules as phorbol esters, saccharin, and cyclamate, it is suggested that they operate through the activation of a hormone response control unit.</jats:p> Saccharin and cyclamate inhibit binding of epidermal growth factor. Proceedings of the National Academy of Sciences |
| spellingShingle | Lee, L S, Proceedings of the National Academy of Sciences, Saccharin and cyclamate inhibit binding of epidermal growth factor., Multidisciplinary |
| title | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_full | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_fullStr | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_full_unstemmed | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_short | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_sort | saccharin and cyclamate inhibit binding of epidermal growth factor. |
| title_unstemmed | Saccharin and cyclamate inhibit binding of epidermal growth factor. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.78.2.1042 |