Eintrag weiter verarbeiten
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen.
Gespeichert in:
Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
---|---|
Personen und Körperschaften: | , |
In: | Proceedings of the National Academy of Sciences, 76, 1979, 2, S. 755-759 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Proceedings of the National Academy of Sciences
|
Schlagwörter: |
author_facet |
Calvet, J P Pederson, T Calvet, J P Pederson, T |
---|---|
author |
Calvet, J P Pederson, T |
spellingShingle |
Calvet, J P Pederson, T Proceedings of the National Academy of Sciences Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. Multidisciplinary |
author_sort |
calvet, j p |
spelling |
Calvet, J P Pederson, T 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.76.2.755 <jats:p>The psoralen derivative aminomethyltrioxsalen (AMT, 4'-aminomethyl-4,5',8-trimethylpsoralen) has been employed as a probe for heterogeneous nuclear RNA (hnRNA) double-stranded regions in experiments with living HeLa cells. hnRNA ribonucleoprotein (hnRNP) particles were purified from untreated or AMT-treated cells after irradiation with 365-nm light, and double-stranded hnRNA regions (dsRNA) were isolated by RNase A + T1 digestion of hnRNP, followed by preparative Cs2SO4 isopycnic centrifugation. The purified, hnRNP-derived dsRNA was then assayed for interstrand crosslinks by measurement of its "snapback" to RNase-resistant form after thermal denaturation. By this procedure, the amount of crosslinked dsRNA was found to be increased 3- to 7-fold in cells exposed to AMT in vivo. The levels of crosslinking in vivo compared favorably with those observed in model experiments with pure dsRNA in vitro. These results establish that double-stranded hnRNA regions exist in the living cell, and they further demonstrate that these base-paired regions are organized as rather accessible sites within the nucleus.</jats:p> Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. Proceedings of the National Academy of Sciences |
doi_str_mv |
10.1073/pnas.76.2.755 |
facet_avail |
Online Free |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjc2LjIuNzU1 |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjc2LjIuNzU1 |
institution |
DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 |
imprint |
Proceedings of the National Academy of Sciences, 1979 |
imprint_str_mv |
Proceedings of the National Academy of Sciences, 1979 |
issn |
0027-8424 1091-6490 |
issn_str_mv |
0027-8424 1091-6490 |
language |
English |
mega_collection |
Proceedings of the National Academy of Sciences (CrossRef) |
match_str |
calvet1979heterogeneousnuclearrnadoublestrandedregionsprobedinlivinghelacellsbycrosslinkingwiththepsoralenderivativeaminomethyltrioxsalen |
publishDateSort |
1979 |
publisher |
Proceedings of the National Academy of Sciences |
recordtype |
ai |
record_format |
ai |
series |
Proceedings of the National Academy of Sciences |
source_id |
49 |
title |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_unstemmed |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_full |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_fullStr |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_full_unstemmed |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_short |
Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_sort |
heterogeneous nuclear rna double-stranded regions probed in living hela cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
topic |
Multidisciplinary |
url |
http://dx.doi.org/10.1073/pnas.76.2.755 |
publishDate |
1979 |
physical |
755-759 |
description |
<jats:p>The psoralen derivative aminomethyltrioxsalen (AMT, 4'-aminomethyl-4,5',8-trimethylpsoralen) has been employed as a probe for heterogeneous nuclear RNA (hnRNA) double-stranded regions in experiments with living HeLa cells. hnRNA ribonucleoprotein (hnRNP) particles were purified from untreated or AMT-treated cells after irradiation with 365-nm light, and double-stranded hnRNA regions (dsRNA) were isolated by RNase A + T1 digestion of hnRNP, followed by preparative Cs2SO4 isopycnic centrifugation. The purified, hnRNP-derived dsRNA was then assayed for interstrand crosslinks by measurement of its "snapback" to RNase-resistant form after thermal denaturation. By this procedure, the amount of crosslinked dsRNA was found to be increased 3- to 7-fold in cells exposed to AMT in vivo. The levels of crosslinking in vivo compared favorably with those observed in model experiments with pure dsRNA in vitro. These results establish that double-stranded hnRNA regions exist in the living cell, and they further demonstrate that these base-paired regions are organized as rather accessible sites within the nucleus.</jats:p> |
container_issue |
2 |
container_start_page |
755 |
container_title |
Proceedings of the National Academy of Sciences |
container_volume |
76 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792342872642551810 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T16:42:15.528Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Heterogeneous+nuclear+RNA+double-stranded+regions+probed+in+living+HeLa+cells+by+crosslinking+with+the+psoralen+derivative+aminomethyltrioxsalen.&rft.date=1979-02-01&genre=article&issn=1091-6490&volume=76&issue=2&spage=755&epage=759&pages=755-759&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=Heterogeneous+nuclear+RNA+double-stranded+regions+probed+in+living+HeLa+cells+by+crosslinking+with+the+psoralen+derivative+aminomethyltrioxsalen.&aulast=Pederson&aufirst=T&rft_id=info%3Adoi%2F10.1073%2Fpnas.76.2.755&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792342872642551810 |
author | Calvet, J P, Pederson, T |
author_facet | Calvet, J P, Pederson, T, Calvet, J P, Pederson, T |
author_sort | calvet, j p |
container_issue | 2 |
container_start_page | 755 |
container_title | Proceedings of the National Academy of Sciences |
container_volume | 76 |
description | <jats:p>The psoralen derivative aminomethyltrioxsalen (AMT, 4'-aminomethyl-4,5',8-trimethylpsoralen) has been employed as a probe for heterogeneous nuclear RNA (hnRNA) double-stranded regions in experiments with living HeLa cells. hnRNA ribonucleoprotein (hnRNP) particles were purified from untreated or AMT-treated cells after irradiation with 365-nm light, and double-stranded hnRNA regions (dsRNA) were isolated by RNase A + T1 digestion of hnRNP, followed by preparative Cs2SO4 isopycnic centrifugation. The purified, hnRNP-derived dsRNA was then assayed for interstrand crosslinks by measurement of its "snapback" to RNase-resistant form after thermal denaturation. By this procedure, the amount of crosslinked dsRNA was found to be increased 3- to 7-fold in cells exposed to AMT in vivo. The levels of crosslinking in vivo compared favorably with those observed in model experiments with pure dsRNA in vitro. These results establish that double-stranded hnRNA regions exist in the living cell, and they further demonstrate that these base-paired regions are organized as rather accessible sites within the nucleus.</jats:p> |
doi_str_mv | 10.1073/pnas.76.2.755 |
facet_avail | Online, Free |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjc2LjIuNzU1 |
imprint | Proceedings of the National Academy of Sciences, 1979 |
imprint_str_mv | Proceedings of the National Academy of Sciences, 1979 |
institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
issn | 0027-8424, 1091-6490 |
issn_str_mv | 0027-8424, 1091-6490 |
language | English |
last_indexed | 2024-03-01T16:42:15.528Z |
match_str | calvet1979heterogeneousnuclearrnadoublestrandedregionsprobedinlivinghelacellsbycrosslinkingwiththepsoralenderivativeaminomethyltrioxsalen |
mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
physical | 755-759 |
publishDate | 1979 |
publishDateSort | 1979 |
publisher | Proceedings of the National Academy of Sciences |
record_format | ai |
recordtype | ai |
series | Proceedings of the National Academy of Sciences |
source_id | 49 |
spelling | Calvet, J P Pederson, T 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.76.2.755 <jats:p>The psoralen derivative aminomethyltrioxsalen (AMT, 4'-aminomethyl-4,5',8-trimethylpsoralen) has been employed as a probe for heterogeneous nuclear RNA (hnRNA) double-stranded regions in experiments with living HeLa cells. hnRNA ribonucleoprotein (hnRNP) particles were purified from untreated or AMT-treated cells after irradiation with 365-nm light, and double-stranded hnRNA regions (dsRNA) were isolated by RNase A + T1 digestion of hnRNP, followed by preparative Cs2SO4 isopycnic centrifugation. The purified, hnRNP-derived dsRNA was then assayed for interstrand crosslinks by measurement of its "snapback" to RNase-resistant form after thermal denaturation. By this procedure, the amount of crosslinked dsRNA was found to be increased 3- to 7-fold in cells exposed to AMT in vivo. The levels of crosslinking in vivo compared favorably with those observed in model experiments with pure dsRNA in vitro. These results establish that double-stranded hnRNA regions exist in the living cell, and they further demonstrate that these base-paired regions are organized as rather accessible sites within the nucleus.</jats:p> Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. Proceedings of the National Academy of Sciences |
spellingShingle | Calvet, J P, Pederson, T, Proceedings of the National Academy of Sciences, Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen., Multidisciplinary |
title | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_full | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_fullStr | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_full_unstemmed | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_short | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_sort | heterogeneous nuclear rna double-stranded regions probed in living hela cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
title_unstemmed | Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. |
topic | Multidisciplinary |
url | http://dx.doi.org/10.1073/pnas.76.2.755 |