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Compression and self-entanglement of single DNA molecules under uniform electric field
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 108, 2011, 39, S. 16153-16158 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
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Tang, Jing Du, Ning Doyle, Patrick S. Tang, Jing Du, Ning Doyle, Patrick S. |
|---|---|
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Tang, Jing Du, Ning Doyle, Patrick S. |
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Tang, Jing Du, Ning Doyle, Patrick S. Proceedings of the National Academy of Sciences Compression and self-entanglement of single DNA molecules under uniform electric field Multidisciplinary |
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tang, jing |
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Tang, Jing Du, Ning Doyle, Patrick S. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1105547108 <jats:p> We experimentally study the effects of a uniform electric field on the conformation of single DNA molecules. We demonstrate that a moderate electric field (∼200 V/cm) strongly compresses isolated DNA polymer coils into isotropic globules. Insight into the nature of these compressed states is gained by following the expansion of the molecules back to equilibrium after halting the electric field. We observe two distinct types of expansion modes: a continuous molecular expansion analogous to a compressed spring expanding, and a much slower expansion characterized by two long-lived metastable states. Fluorescence microscopy and stretching experiments reveal that the metastable states are the result of <jats:italic>intramolecular</jats:italic> self-entanglements induced by the electric field. These results have broad importance in DNA separations and single molecule genomics, polymer rheology, and DNA-based nanofabrication. </jats:p> Compression and self-entanglement of single DNA molecules under uniform electric field Proceedings of the National Academy of Sciences |
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| title |
Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_unstemmed |
Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_full |
Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_fullStr |
Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_full_unstemmed |
Compression and self-entanglement of single DNA molecules under uniform electric field |
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Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_sort |
compression and self-entanglement of single dna molecules under uniform electric field |
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Multidisciplinary |
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http://dx.doi.org/10.1073/pnas.1105547108 |
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2011 |
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16153-16158 |
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<jats:p>
We experimentally study the effects of a uniform electric field on the conformation of single DNA molecules. We demonstrate that a moderate electric field (∼200 V/cm) strongly compresses isolated DNA polymer coils into isotropic globules. Insight into the nature of these compressed states is gained by following the expansion of the molecules back to equilibrium after halting the electric field. We observe two distinct types of expansion modes: a continuous molecular expansion analogous to a compressed spring expanding, and a much slower expansion characterized by two long-lived metastable states. Fluorescence microscopy and stretching experiments reveal that the metastable states are the result of
<jats:italic>intramolecular</jats:italic>
self-entanglements induced by the electric field. These results have broad importance in DNA separations and single molecule genomics, polymer rheology, and DNA-based nanofabrication.
</jats:p> |
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| author | Tang, Jing, Du, Ning, Doyle, Patrick S. |
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| description | <jats:p> We experimentally study the effects of a uniform electric field on the conformation of single DNA molecules. We demonstrate that a moderate electric field (∼200 V/cm) strongly compresses isolated DNA polymer coils into isotropic globules. Insight into the nature of these compressed states is gained by following the expansion of the molecules back to equilibrium after halting the electric field. We observe two distinct types of expansion modes: a continuous molecular expansion analogous to a compressed spring expanding, and a much slower expansion characterized by two long-lived metastable states. Fluorescence microscopy and stretching experiments reveal that the metastable states are the result of <jats:italic>intramolecular</jats:italic> self-entanglements induced by the electric field. These results have broad importance in DNA separations and single molecule genomics, polymer rheology, and DNA-based nanofabrication. </jats:p> |
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| spelling | Tang, Jing Du, Ning Doyle, Patrick S. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1105547108 <jats:p> We experimentally study the effects of a uniform electric field on the conformation of single DNA molecules. We demonstrate that a moderate electric field (∼200 V/cm) strongly compresses isolated DNA polymer coils into isotropic globules. Insight into the nature of these compressed states is gained by following the expansion of the molecules back to equilibrium after halting the electric field. We observe two distinct types of expansion modes: a continuous molecular expansion analogous to a compressed spring expanding, and a much slower expansion characterized by two long-lived metastable states. Fluorescence microscopy and stretching experiments reveal that the metastable states are the result of <jats:italic>intramolecular</jats:italic> self-entanglements induced by the electric field. These results have broad importance in DNA separations and single molecule genomics, polymer rheology, and DNA-based nanofabrication. </jats:p> Compression and self-entanglement of single DNA molecules under uniform electric field Proceedings of the National Academy of Sciences |
| spellingShingle | Tang, Jing, Du, Ning, Doyle, Patrick S., Proceedings of the National Academy of Sciences, Compression and self-entanglement of single DNA molecules under uniform electric field, Multidisciplinary |
| title | Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_full | Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_fullStr | Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_full_unstemmed | Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_short | Compression and self-entanglement of single DNA molecules under uniform electric field |
| title_sort | compression and self-entanglement of single dna molecules under uniform electric field |
| title_unstemmed | Compression and self-entanglement of single DNA molecules under uniform electric field |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.1105547108 |