Eintrag weiter verarbeiten
A force-based protein biochip
Gespeichert in:
| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Proceedings of the National Academy of Sciences, 100, 2003, 20, S. 11356-11360 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. |
|---|---|
| author |
Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. |
| spellingShingle |
Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. Proceedings of the National Academy of Sciences A force-based protein biochip Multidisciplinary |
| author_sort |
blank, k. |
| spelling |
Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1934928100 <jats:p>A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand–receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein–protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.</jats:p> A force-based protein biochip Proceedings of the National Academy of Sciences |
| doi_str_mv |
10.1073/pnas.1934928100 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjE5MzQ5MjgxMDA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjE5MzQ5MjgxMDA |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 |
| imprint |
Proceedings of the National Academy of Sciences, 2003 |
| imprint_str_mv |
Proceedings of the National Academy of Sciences, 2003 |
| issn |
0027-8424 1091-6490 |
| issn_str_mv |
0027-8424 1091-6490 |
| language |
English |
| mega_collection |
Proceedings of the National Academy of Sciences (CrossRef) |
| match_str |
blank2003aforcebasedproteinbiochip |
| publishDateSort |
2003 |
| publisher |
Proceedings of the National Academy of Sciences |
| recordtype |
ai |
| record_format |
ai |
| series |
Proceedings of the National Academy of Sciences |
| source_id |
49 |
| title |
A force-based protein biochip |
| title_unstemmed |
A force-based protein biochip |
| title_full |
A force-based protein biochip |
| title_fullStr |
A force-based protein biochip |
| title_full_unstemmed |
A force-based protein biochip |
| title_short |
A force-based protein biochip |
| title_sort |
a force-based protein biochip |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.1934928100 |
| publishDate |
2003 |
| physical |
11356-11360 |
| description |
<jats:p>A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand–receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein–protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.</jats:p> |
| container_issue |
20 |
| container_start_page |
11356 |
| container_title |
Proceedings of the National Academy of Sciences |
| container_volume |
100 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336287519211533 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:58:03.483Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+force-based+protein+biochip&rft.date=2003-09-30&genre=article&issn=1091-6490&volume=100&issue=20&spage=11356&epage=11360&pages=11356-11360&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=A+force-based+protein+biochip&aulast=Gaub&aufirst=H.+E.&rft_id=info%3Adoi%2F10.1073%2Fpnas.1934928100&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336287519211533 |
| author | Blank, K., Mai, T., Gilbert, I., Schiffmann, S., Rankl, J., Zivin, R., Tackney, C., Nicolaus, T., Spinnler, K., Oesterhelt, F., Benoit, M., Clausen-Schaumann, H., Gaub, H. E. |
| author_facet | Blank, K., Mai, T., Gilbert, I., Schiffmann, S., Rankl, J., Zivin, R., Tackney, C., Nicolaus, T., Spinnler, K., Oesterhelt, F., Benoit, M., Clausen-Schaumann, H., Gaub, H. E., Blank, K., Mai, T., Gilbert, I., Schiffmann, S., Rankl, J., Zivin, R., Tackney, C., Nicolaus, T., Spinnler, K., Oesterhelt, F., Benoit, M., Clausen-Schaumann, H., Gaub, H. E. |
| author_sort | blank, k. |
| container_issue | 20 |
| container_start_page | 11356 |
| container_title | Proceedings of the National Academy of Sciences |
| container_volume | 100 |
| description | <jats:p>A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand–receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein–protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.</jats:p> |
| doi_str_mv | 10.1073/pnas.1934928100 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjE5MzQ5MjgxMDA |
| imprint | Proceedings of the National Academy of Sciences, 2003 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 2003 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
| issn | 0027-8424, 1091-6490 |
| issn_str_mv | 0027-8424, 1091-6490 |
| language | English |
| last_indexed | 2024-03-01T14:58:03.483Z |
| match_str | blank2003aforcebasedproteinbiochip |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 11356-11360 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Proceedings of the National Academy of Sciences |
| record_format | ai |
| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
| source_id | 49 |
| spelling | Blank, K. Mai, T. Gilbert, I. Schiffmann, S. Rankl, J. Zivin, R. Tackney, C. Nicolaus, T. Spinnler, K. Oesterhelt, F. Benoit, M. Clausen-Schaumann, H. Gaub, H. E. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1934928100 <jats:p>A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand–receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein–protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.</jats:p> A force-based protein biochip Proceedings of the National Academy of Sciences |
| spellingShingle | Blank, K., Mai, T., Gilbert, I., Schiffmann, S., Rankl, J., Zivin, R., Tackney, C., Nicolaus, T., Spinnler, K., Oesterhelt, F., Benoit, M., Clausen-Schaumann, H., Gaub, H. E., Proceedings of the National Academy of Sciences, A force-based protein biochip, Multidisciplinary |
| title | A force-based protein biochip |
| title_full | A force-based protein biochip |
| title_fullStr | A force-based protein biochip |
| title_full_unstemmed | A force-based protein biochip |
| title_short | A force-based protein biochip |
| title_sort | a force-based protein biochip |
| title_unstemmed | A force-based protein biochip |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.1934928100 |