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Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Proceedings of the National Academy of Sciences, 106, 2009, 9, S. 3053-3058 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. |
|---|---|
| author |
Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. |
| spellingShingle |
Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. Proceedings of the National Academy of Sciences Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes Multidisciplinary |
| author_sort |
sieglaff, douglas h. |
| spelling |
Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0813264106 <jats:p> The discovery and mapping of <jats:italic>cis</jats:italic> -regulatory elements is important for understanding regulation of gene transcription in mosquito vectors of human diseases. Genome sequence data are available for 3 species, <jats:italic>Aedes aegypti</jats:italic> , <jats:italic>Anopheles gambiae</jats:italic> , and <jats:italic>Culex quinquefasciatus</jats:italic> (Diptera: Culicidae), representing 2 subfamilies (Culicinae and Anophelinae) that are estimated to have diverged 145 to 200 million years ago. Comparative genomics tools were used to screen genomic DNA fragments located in the 5′-end flanking regions of orthologous genes. These analyses resulted in the identification of 137 sequences, designated “mosquito motifs,” 7 to 9 nucleotides in length, representing 18 families of putative <jats:italic>cis</jats:italic> -regulatory elements conserved significantly among the 3 species when compared to the fruit fly, <jats:italic>Drosophila melanogaster</jats:italic> . Forty-one of the motifs were implicated previously in experiments as sites for binding transcription factors or functioning in the regulation of mosquito gene expression. Further analyses revealed associations between specific motifs and expression profiles, particularly in those genes that show increased or decreased mRNA abundance in females following a blood meal, and those accumulating transcription products exclusively or preferentially in the midgut, fat bodies, or ovaries. These results validate the methodology and support a relationship between the discovered motifs and the conservation of hematophagy in mosquitoes. </jats:p> Comparative genomics allows the discovery of <i>cis</i> -regulatory elements in mosquitoes Proceedings of the National Academy of Sciences |
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| title |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_unstemmed |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_full |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_fullStr |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_full_unstemmed |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_short |
Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_sort |
comparative genomics allows the discovery of
<i>cis</i>
-regulatory elements in mosquitoes |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0813264106 |
| publishDate |
2009 |
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3053-3058 |
| description |
<jats:p>
The discovery and mapping of
<jats:italic>cis</jats:italic>
-regulatory elements is important for understanding regulation of gene transcription in mosquito vectors of human diseases. Genome sequence data are available for 3 species,
<jats:italic>Aedes aegypti</jats:italic>
,
<jats:italic>Anopheles gambiae</jats:italic>
, and
<jats:italic>Culex quinquefasciatus</jats:italic>
(Diptera: Culicidae), representing 2 subfamilies (Culicinae and Anophelinae) that are estimated to have diverged 145 to 200 million years ago. Comparative genomics tools were used to screen genomic DNA fragments located in the 5′-end flanking regions of orthologous genes. These analyses resulted in the identification of 137 sequences, designated “mosquito motifs,” 7 to 9 nucleotides in length, representing 18 families of putative
<jats:italic>cis</jats:italic>
-regulatory elements conserved significantly among the 3 species when compared to the fruit fly,
<jats:italic>Drosophila melanogaster</jats:italic>
. Forty-one of the motifs were implicated previously in experiments as sites for binding transcription factors or functioning in the regulation of mosquito gene expression. Further analyses revealed associations between specific motifs and expression profiles, particularly in those genes that show increased or decreased mRNA abundance in females following a blood meal, and those accumulating transcription products exclusively or preferentially in the midgut, fat bodies, or ovaries. These results validate the methodology and support a relationship between the discovered motifs and the conservation of hematophagy in mosquitoes.
</jats:p> |
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| author | Sieglaff, Douglas H., Dunn, W. Augustine, Xie, Xiaohui S., Megy, Karyn, Marinotti, Osvaldo, James, Anthony A. |
| author_facet | Sieglaff, Douglas H., Dunn, W. Augustine, Xie, Xiaohui S., Megy, Karyn, Marinotti, Osvaldo, James, Anthony A., Sieglaff, Douglas H., Dunn, W. Augustine, Xie, Xiaohui S., Megy, Karyn, Marinotti, Osvaldo, James, Anthony A. |
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| description | <jats:p> The discovery and mapping of <jats:italic>cis</jats:italic> -regulatory elements is important for understanding regulation of gene transcription in mosquito vectors of human diseases. Genome sequence data are available for 3 species, <jats:italic>Aedes aegypti</jats:italic> , <jats:italic>Anopheles gambiae</jats:italic> , and <jats:italic>Culex quinquefasciatus</jats:italic> (Diptera: Culicidae), representing 2 subfamilies (Culicinae and Anophelinae) that are estimated to have diverged 145 to 200 million years ago. Comparative genomics tools were used to screen genomic DNA fragments located in the 5′-end flanking regions of orthologous genes. These analyses resulted in the identification of 137 sequences, designated “mosquito motifs,” 7 to 9 nucleotides in length, representing 18 families of putative <jats:italic>cis</jats:italic> -regulatory elements conserved significantly among the 3 species when compared to the fruit fly, <jats:italic>Drosophila melanogaster</jats:italic> . Forty-one of the motifs were implicated previously in experiments as sites for binding transcription factors or functioning in the regulation of mosquito gene expression. Further analyses revealed associations between specific motifs and expression profiles, particularly in those genes that show increased or decreased mRNA abundance in females following a blood meal, and those accumulating transcription products exclusively or preferentially in the midgut, fat bodies, or ovaries. These results validate the methodology and support a relationship between the discovered motifs and the conservation of hematophagy in mosquitoes. </jats:p> |
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| imprint_str_mv | Proceedings of the National Academy of Sciences, 2009 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Sieglaff, Douglas H. Dunn, W. Augustine Xie, Xiaohui S. Megy, Karyn Marinotti, Osvaldo James, Anthony A. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0813264106 <jats:p> The discovery and mapping of <jats:italic>cis</jats:italic> -regulatory elements is important for understanding regulation of gene transcription in mosquito vectors of human diseases. Genome sequence data are available for 3 species, <jats:italic>Aedes aegypti</jats:italic> , <jats:italic>Anopheles gambiae</jats:italic> , and <jats:italic>Culex quinquefasciatus</jats:italic> (Diptera: Culicidae), representing 2 subfamilies (Culicinae and Anophelinae) that are estimated to have diverged 145 to 200 million years ago. Comparative genomics tools were used to screen genomic DNA fragments located in the 5′-end flanking regions of orthologous genes. These analyses resulted in the identification of 137 sequences, designated “mosquito motifs,” 7 to 9 nucleotides in length, representing 18 families of putative <jats:italic>cis</jats:italic> -regulatory elements conserved significantly among the 3 species when compared to the fruit fly, <jats:italic>Drosophila melanogaster</jats:italic> . Forty-one of the motifs were implicated previously in experiments as sites for binding transcription factors or functioning in the regulation of mosquito gene expression. Further analyses revealed associations between specific motifs and expression profiles, particularly in those genes that show increased or decreased mRNA abundance in females following a blood meal, and those accumulating transcription products exclusively or preferentially in the midgut, fat bodies, or ovaries. These results validate the methodology and support a relationship between the discovered motifs and the conservation of hematophagy in mosquitoes. </jats:p> Comparative genomics allows the discovery of <i>cis</i> -regulatory elements in mosquitoes Proceedings of the National Academy of Sciences |
| spellingShingle | Sieglaff, Douglas H., Dunn, W. Augustine, Xie, Xiaohui S., Megy, Karyn, Marinotti, Osvaldo, James, Anthony A., Proceedings of the National Academy of Sciences, Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes, Multidisciplinary |
| title | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_full | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_fullStr | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_full_unstemmed | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_short | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| title_sort | comparative genomics allows the discovery of <i>cis</i> -regulatory elements in mosquitoes |
| title_unstemmed | Comparative genomics allows the discovery of cis -regulatory elements in mosquitoes |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0813264106 |