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S4-based voltage sensors have three major conformations
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Proceedings of the National Academy of Sciences, 105, 2008, 46, S. 17600-17607 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco |
|---|---|
| author |
Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco |
| spellingShingle |
Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco Proceedings of the National Academy of Sciences S4-based voltage sensors have three major conformations Multidisciplinary |
| author_sort |
villalba-galea, carlos a. |
| spelling |
Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0807387105 <jats:p> Voltage sensors containing the charged S4 membrane segment display a gating charge vs. voltage ( <jats:italic>Q–V</jats:italic> ) curve that depends on the initial voltage. The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows the same phenomenon and the <jats:italic>Q–V</jats:italic> recorded with a depolarized initial voltage is more stable by at least 3 <jats:italic>RT</jats:italic> . The leftward shift of the <jats:italic>Q–V</jats:italic> curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements. The fluorescence shows two components: one that traces the time course of the charge movement between the resting and active states and a slower component that traces the transition between the active state and a more stable state we call the relaxed state. Temperature dependence shows a large negative enthalpic change when going from the active to the relaxed state that is almost compensated by a large negative entropic change. The <jats:italic>Q–V</jats:italic> curve midpoint measured for pulses that move the sensor between the resting and active states, but not long enough to evolve into the relaxed states, show a periodicity of 120°, indicating a 3 <jats:sub>10</jats:sub> secondary structure of the S4 segment when determined under histidine scanning. We hypothesize that the S4 segment moves as a 3 <jats:sub>10</jats:sub> helix between the resting and active states and that it converts to an α-helix when evolving into the relaxed state, which is most likely to be the state captured in the crystal structures. </jats:p> S4-based voltage sensors have three major conformations Proceedings of the National Academy of Sciences |
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2008 |
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Proceedings of the National Academy of Sciences |
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| title |
S4-based voltage sensors have three major conformations |
| title_unstemmed |
S4-based voltage sensors have three major conformations |
| title_full |
S4-based voltage sensors have three major conformations |
| title_fullStr |
S4-based voltage sensors have three major conformations |
| title_full_unstemmed |
S4-based voltage sensors have three major conformations |
| title_short |
S4-based voltage sensors have three major conformations |
| title_sort |
s4-based voltage sensors have three major conformations |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0807387105 |
| publishDate |
2008 |
| physical |
17600-17607 |
| description |
<jats:p>
Voltage sensors containing the charged S4 membrane segment display a gating charge vs. voltage (
<jats:italic>Q–V</jats:italic>
) curve that depends on the initial voltage. The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows the same phenomenon and the
<jats:italic>Q–V</jats:italic>
recorded with a depolarized initial voltage is more stable by at least 3
<jats:italic>RT</jats:italic>
. The leftward shift of the
<jats:italic>Q–V</jats:italic>
curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements. The fluorescence shows two components: one that traces the time course of the charge movement between the resting and active states and a slower component that traces the transition between the active state and a more stable state we call the relaxed state. Temperature dependence shows a large negative enthalpic change when going from the active to the relaxed state that is almost compensated by a large negative entropic change. The
<jats:italic>Q–V</jats:italic>
curve midpoint measured for pulses that move the sensor between the resting and active states, but not long enough to evolve into the relaxed states, show a periodicity of 120°, indicating a 3
<jats:sub>10</jats:sub>
secondary structure of the S4 segment when determined under histidine scanning. We hypothesize that the S4 segment moves as a 3
<jats:sub>10</jats:sub>
helix between the resting and active states and that it converts to an α-helix when evolving into the relaxed state, which is most likely to be the state captured in the crystal structures.
</jats:p> |
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| author | Villalba-Galea, Carlos A., Sandtner, Walter, Starace, Dorine M., Bezanilla, Francisco |
| author_facet | Villalba-Galea, Carlos A., Sandtner, Walter, Starace, Dorine M., Bezanilla, Francisco, Villalba-Galea, Carlos A., Sandtner, Walter, Starace, Dorine M., Bezanilla, Francisco |
| author_sort | villalba-galea, carlos a. |
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| container_title | Proceedings of the National Academy of Sciences |
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| description | <jats:p> Voltage sensors containing the charged S4 membrane segment display a gating charge vs. voltage ( <jats:italic>Q–V</jats:italic> ) curve that depends on the initial voltage. The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows the same phenomenon and the <jats:italic>Q–V</jats:italic> recorded with a depolarized initial voltage is more stable by at least 3 <jats:italic>RT</jats:italic> . The leftward shift of the <jats:italic>Q–V</jats:italic> curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements. The fluorescence shows two components: one that traces the time course of the charge movement between the resting and active states and a slower component that traces the transition between the active state and a more stable state we call the relaxed state. Temperature dependence shows a large negative enthalpic change when going from the active to the relaxed state that is almost compensated by a large negative entropic change. The <jats:italic>Q–V</jats:italic> curve midpoint measured for pulses that move the sensor between the resting and active states, but not long enough to evolve into the relaxed states, show a periodicity of 120°, indicating a 3 <jats:sub>10</jats:sub> secondary structure of the S4 segment when determined under histidine scanning. We hypothesize that the S4 segment moves as a 3 <jats:sub>10</jats:sub> helix between the resting and active states and that it converts to an α-helix when evolving into the relaxed state, which is most likely to be the state captured in the crystal structures. </jats:p> |
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| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105 |
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| language | English |
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| spelling | Villalba-Galea, Carlos A. Sandtner, Walter Starace, Dorine M. Bezanilla, Francisco 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0807387105 <jats:p> Voltage sensors containing the charged S4 membrane segment display a gating charge vs. voltage ( <jats:italic>Q–V</jats:italic> ) curve that depends on the initial voltage. The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows the same phenomenon and the <jats:italic>Q–V</jats:italic> recorded with a depolarized initial voltage is more stable by at least 3 <jats:italic>RT</jats:italic> . The leftward shift of the <jats:italic>Q–V</jats:italic> curve under prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed fluorescence measurements. The fluorescence shows two components: one that traces the time course of the charge movement between the resting and active states and a slower component that traces the transition between the active state and a more stable state we call the relaxed state. Temperature dependence shows a large negative enthalpic change when going from the active to the relaxed state that is almost compensated by a large negative entropic change. The <jats:italic>Q–V</jats:italic> curve midpoint measured for pulses that move the sensor between the resting and active states, but not long enough to evolve into the relaxed states, show a periodicity of 120°, indicating a 3 <jats:sub>10</jats:sub> secondary structure of the S4 segment when determined under histidine scanning. We hypothesize that the S4 segment moves as a 3 <jats:sub>10</jats:sub> helix between the resting and active states and that it converts to an α-helix when evolving into the relaxed state, which is most likely to be the state captured in the crystal structures. </jats:p> S4-based voltage sensors have three major conformations Proceedings of the National Academy of Sciences |
| spellingShingle | Villalba-Galea, Carlos A., Sandtner, Walter, Starace, Dorine M., Bezanilla, Francisco, Proceedings of the National Academy of Sciences, S4-based voltage sensors have three major conformations, Multidisciplinary |
| title | S4-based voltage sensors have three major conformations |
| title_full | S4-based voltage sensors have three major conformations |
| title_fullStr | S4-based voltage sensors have three major conformations |
| title_full_unstemmed | S4-based voltage sensors have three major conformations |
| title_short | S4-based voltage sensors have three major conformations |
| title_sort | s4-based voltage sensors have three major conformations |
| title_unstemmed | S4-based voltage sensors have three major conformations |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0807387105 |