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Backscattering from a tethered bead as a probe of DNA flexibility
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Zeitschriftentitel: | Applied Physics Letters |
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Personen und Körperschaften: | , , |
In: | Applied Physics Letters, 73, 1998, 3, S. 291-293 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
AIP Publishing
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Schlagwörter: |
author_facet |
Shivashankar, G. V. Stolovitzky, G. Libchaber, A. Shivashankar, G. V. Stolovitzky, G. Libchaber, A. |
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author |
Shivashankar, G. V. Stolovitzky, G. Libchaber, A. |
spellingShingle |
Shivashankar, G. V. Stolovitzky, G. Libchaber, A. Applied Physics Letters Backscattering from a tethered bead as a probe of DNA flexibility Physics and Astronomy (miscellaneous) |
author_sort |
shivashankar, g. v. |
spelling |
Shivashankar, G. V. Stolovitzky, G. Libchaber, A. 0003-6951 1077-3118 AIP Publishing Physics and Astronomy (miscellaneous) http://dx.doi.org/10.1063/1.121798 <jats:p>Using an optical tweezer, we trap a DNA polymer tethered bead. Collinear with the tweezer, a red laser light beam probes the bead fluctuations through backscattered radiation measurement. Those fluctuations give information on the flexibility of DNA and can be used to probe the interaction of proteins with DNA. We use this approach to directly monitor the extension of a single DNA polymer beyond its contour length induced by the polymerization of Rec A protein on DNA.</jats:p> Backscattering from a tethered bead as a probe of DNA flexibility Applied Physics Letters |
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1998 |
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AIP Publishing |
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Applied Physics Letters |
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title |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_unstemmed |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_full |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_fullStr |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_full_unstemmed |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_short |
Backscattering from a tethered bead as a probe of DNA flexibility |
title_sort |
backscattering from a tethered bead as a probe of dna flexibility |
topic |
Physics and Astronomy (miscellaneous) |
url |
http://dx.doi.org/10.1063/1.121798 |
publishDate |
1998 |
physical |
291-293 |
description |
<jats:p>Using an optical tweezer, we trap a DNA polymer tethered bead. Collinear with the tweezer, a red laser light beam probes the bead fluctuations through backscattered radiation measurement. Those fluctuations give information on the flexibility of DNA and can be used to probe the interaction of proteins with DNA. We use this approach to directly monitor the extension of a single DNA polymer beyond its contour length induced by the polymerization of Rec A protein on DNA.</jats:p> |
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author | Shivashankar, G. V., Stolovitzky, G., Libchaber, A. |
author_facet | Shivashankar, G. V., Stolovitzky, G., Libchaber, A., Shivashankar, G. V., Stolovitzky, G., Libchaber, A. |
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container_title | Applied Physics Letters |
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description | <jats:p>Using an optical tweezer, we trap a DNA polymer tethered bead. Collinear with the tweezer, a red laser light beam probes the bead fluctuations through backscattered radiation measurement. Those fluctuations give information on the flexibility of DNA and can be used to probe the interaction of proteins with DNA. We use this approach to directly monitor the extension of a single DNA polymer beyond its contour length induced by the polymerization of Rec A protein on DNA.</jats:p> |
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institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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spelling | Shivashankar, G. V. Stolovitzky, G. Libchaber, A. 0003-6951 1077-3118 AIP Publishing Physics and Astronomy (miscellaneous) http://dx.doi.org/10.1063/1.121798 <jats:p>Using an optical tweezer, we trap a DNA polymer tethered bead. Collinear with the tweezer, a red laser light beam probes the bead fluctuations through backscattered radiation measurement. Those fluctuations give information on the flexibility of DNA and can be used to probe the interaction of proteins with DNA. We use this approach to directly monitor the extension of a single DNA polymer beyond its contour length induced by the polymerization of Rec A protein on DNA.</jats:p> Backscattering from a tethered bead as a probe of DNA flexibility Applied Physics Letters |
spellingShingle | Shivashankar, G. V., Stolovitzky, G., Libchaber, A., Applied Physics Letters, Backscattering from a tethered bead as a probe of DNA flexibility, Physics and Astronomy (miscellaneous) |
title | Backscattering from a tethered bead as a probe of DNA flexibility |
title_full | Backscattering from a tethered bead as a probe of DNA flexibility |
title_fullStr | Backscattering from a tethered bead as a probe of DNA flexibility |
title_full_unstemmed | Backscattering from a tethered bead as a probe of DNA flexibility |
title_short | Backscattering from a tethered bead as a probe of DNA flexibility |
title_sort | backscattering from a tethered bead as a probe of dna flexibility |
title_unstemmed | Backscattering from a tethered bead as a probe of DNA flexibility |
topic | Physics and Astronomy (miscellaneous) |
url | http://dx.doi.org/10.1063/1.121798 |