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Cloning and characterization of a relA/spoT homologue from Bacillus subtilis
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| Zeitschriftentitel: | Molecular Microbiology |
|---|---|
| Personen und Körperschaften: | , |
| In: | Molecular Microbiology, 26, 1997, 1, S. 65-79 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Wendrich, Thomas M. Marahiel, Mohamed A. Wendrich, Thomas M. Marahiel, Mohamed A. |
|---|---|
| author |
Wendrich, Thomas M. Marahiel, Mohamed A. |
| spellingShingle |
Wendrich, Thomas M. Marahiel, Mohamed A. Molecular Microbiology Cloning and characterization of a relA/spoT homologue from Bacillus subtilis Molecular Biology Microbiology |
| author_sort |
wendrich, thomas m. |
| spelling |
Wendrich, Thomas M. Marahiel, Mohamed A. 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1046/j.1365-2958.1997.5511919.x <jats:p>A PCR‐amplified DNA fragment of the <jats:italic>relA</jats:italic> gene from genomic <jats:italic>Bacillus subtilis</jats:italic> DNA was used to isolate the entire <jats:italic>relA</jats:italic>/<jats:italic>spoT</jats:italic> homologue and two adjacent open reading frames (ORFs) from a λ ZAP Express library. The <jats:italic>relA</jats:italic> gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170 aa) that shares high similarity to adenine phosphoribosyltransferase genes (<jats:italic>apt</jats:italic>), and downstream by an ORF (131 aa) of unknown function. This genetic organization is similar to that in <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) and <jats:italic>Streptococcus equismilis</jats:italic> H46A. <jats:italic>relA</jats:italic> shows significant similarity to the <jats:italic>Escherichia coli relA</jats:italic> and <jats:italic>spoT</jats:italic> genes, which are responsible for the synthesis and degradation of the highly phosphorylated guanosine nucleotides (p)ppGpp, triggering the stringent response. Deletion of the <jats:italic>relA</jats:italic> gene generated a (p)ppGpp<jats:sup>0</jats:sup> phenotype that demonstrated its essential role in the response to amino acid deprivation and resulted in impaired/lowered induction of proteins involved in stress response as well as amino acid biosynthesis, as judged by two‐dimensional gel electrophoresis. The same effects of impaired induction of some σ<jats:sup>B</jats:sup>‐independent proteins could also be shown in a <jats:italic>sigB/relA</jats:italic> double mutant, supporting the role of <jats:italic>relA</jats:italic> in derepression/induction of catabolic and anabolic genes during stringent response.</jats:p> Cloning and characterization of a <i>relA</i>/<i>spoT</i> homologue from <i>Bacillus subtilis</i> Molecular Microbiology |
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10.1046/j.1365-2958.1997.5511919.x |
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Biologie |
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Wiley, 1997 |
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Wiley, 1997 |
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1997 |
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Molecular Microbiology |
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49 |
| title |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_unstemmed |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_full |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_fullStr |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_full_unstemmed |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_short |
Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_sort |
cloning and characterization of a <i>rela</i>/<i>spot</i> homologue from <i>bacillus subtilis</i> |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1046/j.1365-2958.1997.5511919.x |
| publishDate |
1997 |
| physical |
65-79 |
| description |
<jats:p>A PCR‐amplified DNA fragment of the <jats:italic>relA</jats:italic> gene from genomic <jats:italic>Bacillus subtilis</jats:italic> DNA was used to isolate the entire <jats:italic>relA</jats:italic>/<jats:italic>spoT</jats:italic> homologue and two adjacent open reading frames (ORFs) from a λ ZAP Express library. The <jats:italic>relA</jats:italic> gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170 aa) that shares high similarity to adenine phosphoribosyltransferase genes (<jats:italic>apt</jats:italic>), and downstream by an ORF (131 aa) of unknown function. This genetic organization is similar to that in <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) and <jats:italic>Streptococcus equismilis</jats:italic> H46A. <jats:italic>relA</jats:italic> shows significant similarity to the <jats:italic>Escherichia coli relA</jats:italic> and <jats:italic>spoT</jats:italic> genes, which are responsible for the synthesis and degradation of the highly phosphorylated guanosine nucleotides (p)ppGpp, triggering the stringent response. Deletion of the <jats:italic>relA</jats:italic> gene generated a (p)ppGpp<jats:sup>0</jats:sup> phenotype that demonstrated its essential role in the response to amino acid deprivation and resulted in impaired/lowered induction of proteins involved in stress response as well as amino acid biosynthesis, as judged by two‐dimensional gel electrophoresis. The same effects of impaired induction of some σ<jats:sup>B</jats:sup>‐independent proteins could also be shown in a <jats:italic>sigB/relA</jats:italic> double mutant, supporting the role of <jats:italic>relA</jats:italic> in derepression/induction of catabolic and anabolic genes during stringent response.</jats:p> |
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| author | Wendrich, Thomas M., Marahiel, Mohamed A. |
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| description | <jats:p>A PCR‐amplified DNA fragment of the <jats:italic>relA</jats:italic> gene from genomic <jats:italic>Bacillus subtilis</jats:italic> DNA was used to isolate the entire <jats:italic>relA</jats:italic>/<jats:italic>spoT</jats:italic> homologue and two adjacent open reading frames (ORFs) from a λ ZAP Express library. The <jats:italic>relA</jats:italic> gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170 aa) that shares high similarity to adenine phosphoribosyltransferase genes (<jats:italic>apt</jats:italic>), and downstream by an ORF (131 aa) of unknown function. This genetic organization is similar to that in <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) and <jats:italic>Streptococcus equismilis</jats:italic> H46A. <jats:italic>relA</jats:italic> shows significant similarity to the <jats:italic>Escherichia coli relA</jats:italic> and <jats:italic>spoT</jats:italic> genes, which are responsible for the synthesis and degradation of the highly phosphorylated guanosine nucleotides (p)ppGpp, triggering the stringent response. Deletion of the <jats:italic>relA</jats:italic> gene generated a (p)ppGpp<jats:sup>0</jats:sup> phenotype that demonstrated its essential role in the response to amino acid deprivation and resulted in impaired/lowered induction of proteins involved in stress response as well as amino acid biosynthesis, as judged by two‐dimensional gel electrophoresis. The same effects of impaired induction of some σ<jats:sup>B</jats:sup>‐independent proteins could also be shown in a <jats:italic>sigB/relA</jats:italic> double mutant, supporting the role of <jats:italic>relA</jats:italic> in derepression/induction of catabolic and anabolic genes during stringent response.</jats:p> |
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| imprint | Wiley, 1997 |
| imprint_str_mv | Wiley, 1997 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
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| physical | 65-79 |
| publishDate | 1997 |
| publishDateSort | 1997 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Molecular Microbiology |
| source_id | 49 |
| spelling | Wendrich, Thomas M. Marahiel, Mohamed A. 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1046/j.1365-2958.1997.5511919.x <jats:p>A PCR‐amplified DNA fragment of the <jats:italic>relA</jats:italic> gene from genomic <jats:italic>Bacillus subtilis</jats:italic> DNA was used to isolate the entire <jats:italic>relA</jats:italic>/<jats:italic>spoT</jats:italic> homologue and two adjacent open reading frames (ORFs) from a λ ZAP Express library. The <jats:italic>relA</jats:italic> gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170 aa) that shares high similarity to adenine phosphoribosyltransferase genes (<jats:italic>apt</jats:italic>), and downstream by an ORF (131 aa) of unknown function. This genetic organization is similar to that in <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) and <jats:italic>Streptococcus equismilis</jats:italic> H46A. <jats:italic>relA</jats:italic> shows significant similarity to the <jats:italic>Escherichia coli relA</jats:italic> and <jats:italic>spoT</jats:italic> genes, which are responsible for the synthesis and degradation of the highly phosphorylated guanosine nucleotides (p)ppGpp, triggering the stringent response. Deletion of the <jats:italic>relA</jats:italic> gene generated a (p)ppGpp<jats:sup>0</jats:sup> phenotype that demonstrated its essential role in the response to amino acid deprivation and resulted in impaired/lowered induction of proteins involved in stress response as well as amino acid biosynthesis, as judged by two‐dimensional gel electrophoresis. The same effects of impaired induction of some σ<jats:sup>B</jats:sup>‐independent proteins could also be shown in a <jats:italic>sigB/relA</jats:italic> double mutant, supporting the role of <jats:italic>relA</jats:italic> in derepression/induction of catabolic and anabolic genes during stringent response.</jats:p> Cloning and characterization of a <i>relA</i>/<i>spoT</i> homologue from <i>Bacillus subtilis</i> Molecular Microbiology |
| spellingShingle | Wendrich, Thomas M., Marahiel, Mohamed A., Molecular Microbiology, Cloning and characterization of a relA/spoT homologue from Bacillus subtilis, Molecular Biology, Microbiology |
| title | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_full | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_fullStr | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_full_unstemmed | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_short | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| title_sort | cloning and characterization of a <i>rela</i>/<i>spot</i> homologue from <i>bacillus subtilis</i> |
| title_unstemmed | Cloning and characterization of a relA/spoT homologue from Bacillus subtilis |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1046/j.1365-2958.1997.5511919.x |