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Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2)
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| Zeitschriftentitel: | Molecular Microbiology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Microbiology, 19, 1996, 2, S. 357-368 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn |
|---|---|
| author |
Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn |
| spellingShingle |
Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn Molecular Microbiology Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) Molecular Biology Microbiology |
| author_sort |
chakraburtty, rekha |
| spelling |
Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1046/j.1365-2958.1996.390919.x <jats:p>An internal segment of the (p)ppGpp synthetase gene, <jats:italic>relA</jats:italic>, of <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. <jats:italic>relA</jats:italic> lies downstream of a gene (<jats:italic>apt</jats:italic>) that apparently encodes adenine phosphoribosyltransferase and is transcribed from two promoters, <jats:italic>relA</jats:italic>p1 and <jats:italic>relA</jats:italic>p2, and by transcriptional readthrough from <jats:italic>apt</jats:italic>. While the level of <jats:italic>relA</jats:italic>p2 transcripts remained relatively constant, <jats:italic>relA</jats:italic>p1 activity apparently peaked during transition phase, following a decline in readthrough transcription from <jats:italic>apt</jats:italic>. Disruption of <jats:italic>relA</jats:italic> using an <jats:italic>att</jats:italic><jats:sup>−</jats:sup> derivative of the temperate phage φC31 abolished ppGpp synthesis on amino acid depletion. When grown on agar, the disruptants grew more slowly than a control lysogen made with an <jats:italic>att</jats:italic><jats:sup>+</jats:sup> phage vector and gave smaller colonies that sporulated normally. The <jats:italic>relA</jats:italic> mutation had no consistent or marked effect on actinorhodin production in either liquid‐ or agar‐grown cultures, indicating that elevated levels of (p)ppGpp are not essential for triggering the onset of antibiotic production.</jats:p> Cloning, characterization and disruption of a (p)ppGpp synthetase gene (<i>relA</i>) of <i>Streptomyces coelicolor</i> A3(2) Molecular Microbiology |
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10.1046/j.1365-2958.1996.390919.x |
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Biologie |
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Wiley, 1996 |
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chakraburtty1996cloningcharacterizationanddisruptionofapppgppsynthetasegenerelaofstreptomycescoelicolora32 |
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1996 |
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Molecular Microbiology |
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49 |
| title |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_unstemmed |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_full |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_fullStr |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_full_unstemmed |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_short |
Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_sort |
cloning, characterization and disruption of a (p)ppgpp synthetase gene (<i>rela</i>) of <i>streptomyces coelicolor</i> a3(2) |
| topic |
Molecular Biology Microbiology |
| url |
http://dx.doi.org/10.1046/j.1365-2958.1996.390919.x |
| publishDate |
1996 |
| physical |
357-368 |
| description |
<jats:p>An internal segment of the (p)ppGpp synthetase gene, <jats:italic>relA</jats:italic>, of <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. <jats:italic>relA</jats:italic> lies downstream of a gene (<jats:italic>apt</jats:italic>) that apparently encodes adenine phosphoribosyltransferase and is transcribed from two promoters, <jats:italic>relA</jats:italic>p1 and <jats:italic>relA</jats:italic>p2, and by transcriptional readthrough from <jats:italic>apt</jats:italic>. While the level of <jats:italic>relA</jats:italic>p2 transcripts remained relatively constant, <jats:italic>relA</jats:italic>p1 activity apparently peaked during transition phase, following a decline in readthrough transcription from <jats:italic>apt</jats:italic>. Disruption of <jats:italic>relA</jats:italic> using an <jats:italic>att</jats:italic><jats:sup>−</jats:sup> derivative of the temperate phage φC31 abolished ppGpp synthesis on amino acid depletion. When grown on agar, the disruptants grew more slowly than a control lysogen made with an <jats:italic>att</jats:italic><jats:sup>+</jats:sup> phage vector and gave smaller colonies that sporulated normally. The <jats:italic>relA</jats:italic> mutation had no consistent or marked effect on actinorhodin production in either liquid‐ or agar‐grown cultures, indicating that elevated levels of (p)ppGpp are not essential for triggering the onset of antibiotic production.</jats:p> |
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| author | Chakraburtty, Rekha, White, Janet, Takano, Eriko, Bibb, Mervyn |
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| description | <jats:p>An internal segment of the (p)ppGpp synthetase gene, <jats:italic>relA</jats:italic>, of <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. <jats:italic>relA</jats:italic> lies downstream of a gene (<jats:italic>apt</jats:italic>) that apparently encodes adenine phosphoribosyltransferase and is transcribed from two promoters, <jats:italic>relA</jats:italic>p1 and <jats:italic>relA</jats:italic>p2, and by transcriptional readthrough from <jats:italic>apt</jats:italic>. While the level of <jats:italic>relA</jats:italic>p2 transcripts remained relatively constant, <jats:italic>relA</jats:italic>p1 activity apparently peaked during transition phase, following a decline in readthrough transcription from <jats:italic>apt</jats:italic>. Disruption of <jats:italic>relA</jats:italic> using an <jats:italic>att</jats:italic><jats:sup>−</jats:sup> derivative of the temperate phage φC31 abolished ppGpp synthesis on amino acid depletion. When grown on agar, the disruptants grew more slowly than a control lysogen made with an <jats:italic>att</jats:italic><jats:sup>+</jats:sup> phage vector and gave smaller colonies that sporulated normally. The <jats:italic>relA</jats:italic> mutation had no consistent or marked effect on actinorhodin production in either liquid‐ or agar‐grown cultures, indicating that elevated levels of (p)ppGpp are not essential for triggering the onset of antibiotic production.</jats:p> |
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| imprint | Wiley, 1996 |
| imprint_str_mv | Wiley, 1996 |
| institution | DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1 |
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| mega_collection | Wiley (CrossRef) |
| physical | 357-368 |
| publishDate | 1996 |
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| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Molecular Microbiology |
| source_id | 49 |
| spelling | Chakraburtty, Rekha White, Janet Takano, Eriko Bibb, Mervyn 0950-382X 1365-2958 Wiley Molecular Biology Microbiology http://dx.doi.org/10.1046/j.1365-2958.1996.390919.x <jats:p>An internal segment of the (p)ppGpp synthetase gene, <jats:italic>relA</jats:italic>, of <jats:italic>Streptomyces coelicolor</jats:italic> A3(2) was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. <jats:italic>relA</jats:italic> lies downstream of a gene (<jats:italic>apt</jats:italic>) that apparently encodes adenine phosphoribosyltransferase and is transcribed from two promoters, <jats:italic>relA</jats:italic>p1 and <jats:italic>relA</jats:italic>p2, and by transcriptional readthrough from <jats:italic>apt</jats:italic>. While the level of <jats:italic>relA</jats:italic>p2 transcripts remained relatively constant, <jats:italic>relA</jats:italic>p1 activity apparently peaked during transition phase, following a decline in readthrough transcription from <jats:italic>apt</jats:italic>. Disruption of <jats:italic>relA</jats:italic> using an <jats:italic>att</jats:italic><jats:sup>−</jats:sup> derivative of the temperate phage φC31 abolished ppGpp synthesis on amino acid depletion. When grown on agar, the disruptants grew more slowly than a control lysogen made with an <jats:italic>att</jats:italic><jats:sup>+</jats:sup> phage vector and gave smaller colonies that sporulated normally. The <jats:italic>relA</jats:italic> mutation had no consistent or marked effect on actinorhodin production in either liquid‐ or agar‐grown cultures, indicating that elevated levels of (p)ppGpp are not essential for triggering the onset of antibiotic production.</jats:p> Cloning, characterization and disruption of a (p)ppGpp synthetase gene (<i>relA</i>) of <i>Streptomyces coelicolor</i> A3(2) Molecular Microbiology |
| spellingShingle | Chakraburtty, Rekha, White, Janet, Takano, Eriko, Bibb, Mervyn, Molecular Microbiology, Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2), Molecular Biology, Microbiology |
| title | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_full | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_fullStr | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_full_unstemmed | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_short | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| title_sort | cloning, characterization and disruption of a (p)ppgpp synthetase gene (<i>rela</i>) of <i>streptomyces coelicolor</i> a3(2) |
| title_unstemmed | Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) |
| topic | Molecular Biology, Microbiology |
| url | http://dx.doi.org/10.1046/j.1365-2958.1996.390919.x |