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Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐...
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| Zeitschriftentitel: | British Journal of Haematology |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | British Journal of Haematology, 113, 2001, 4, S. 1072-1075 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. |
|---|---|
| author |
Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. |
| spellingShingle |
Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. British Journal of Haematology Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method Hematology |
| author_sort |
elmaagacli, ahmet h. |
| spelling |
Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. 0007-1048 1365-2141 Wiley Hematology http://dx.doi.org/10.1046/j.1365-2141.2001.02858.x <jats:p>We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real‐time RT‐PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR‐ABL amount differed significantly (<jats:italic>P</jats:italic> < 0·001) between the various disease stages and was 0·06% (range 0·001–1·55%), 3·2% (range 1·4–5·6%) and 21·5% (range 6·8 −827%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.</jats:p> Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method British Journal of Haematology |
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| title |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_unstemmed |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_full |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_fullStr |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_full_unstemmed |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_short |
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_sort |
estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of bcr‐abl fusion transcripts detected using a new real‐time polymerase chain reaction method |
| topic |
Hematology |
| url |
http://dx.doi.org/10.1046/j.1365-2141.2001.02858.x |
| publishDate |
2001 |
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1072-1075 |
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<jats:p>We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real‐time RT‐PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR‐ABL amount differed significantly (<jats:italic>P</jats:italic> < 0·001) between the various disease stages and was 0·06% (range 0·001–1·55%), 3·2% (range 1·4–5·6%) and 21·5% (range 6·8 −827%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.</jats:p> |
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| author_facet | Elmaagacli, Ahmet H., Freist, Annette, Hahn, Meinhard, Opalka, Bertram, Seeber, Siegfried, Schaefer, Ulrich W., Beelen, D. W., Elmaagacli, Ahmet H., Freist, Annette, Hahn, Meinhard, Opalka, Bertram, Seeber, Siegfried, Schaefer, Ulrich W., Beelen, D. W. |
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| description | <jats:p>We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real‐time RT‐PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR‐ABL amount differed significantly (<jats:italic>P</jats:italic> < 0·001) between the various disease stages and was 0·06% (range 0·001–1·55%), 3·2% (range 1·4–5·6%) and 21·5% (range 6·8 −827%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.</jats:p> |
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| spelling | Elmaagacli, Ahmet H. Freist, Annette Hahn, Meinhard Opalka, Bertram Seeber, Siegfried Schaefer, Ulrich W. Beelen, D. W. 0007-1048 1365-2141 Wiley Hematology http://dx.doi.org/10.1046/j.1365-2141.2001.02858.x <jats:p>We have used a new single‐step real‐time reverse transcription polymerase chain reaction (RT‐PCR) method to quantify BCR‐ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR‐ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real‐time RT‐PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR‐ABL amount differed significantly (<jats:italic>P</jats:italic> < 0·001) between the various disease stages and was 0·06% (range 0·001–1·55%), 3·2% (range 1·4–5·6%) and 21·5% (range 6·8 −827%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.</jats:p> Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method British Journal of Haematology |
| spellingShingle | Elmaagacli, Ahmet H., Freist, Annette, Hahn, Meinhard, Opalka, Bertram, Seeber, Siegfried, Schaefer, Ulrich W., Beelen, D. W., British Journal of Haematology, Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method, Hematology |
| title | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_full | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_fullStr | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_full_unstemmed | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_short | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_sort | estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of bcr‐abl fusion transcripts detected using a new real‐time polymerase chain reaction method |
| title_unstemmed | Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR‐ABL fusion transcripts detected using a new real‐time polymerase chain reaction method |
| topic | Hematology |
| url | http://dx.doi.org/10.1046/j.1365-2141.2001.02858.x |