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Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation
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| Zeitschriftentitel: | European Journal of Neuroscience |
|---|---|
| Personen und Körperschaften: | , , |
| In: | European Journal of Neuroscience, 15, 2002, 10, S. 1603-1614 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Meuth, Sven Pape, Hans‐Christian Budde, Thomas Meuth, Sven Pape, Hans‐Christian Budde, Thomas |
|---|---|
| author |
Meuth, Sven Pape, Hans‐Christian Budde, Thomas |
| spellingShingle |
Meuth, Sven Pape, Hans‐Christian Budde, Thomas European Journal of Neuroscience Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation General Neuroscience |
| author_sort |
meuth, sven |
| spelling |
Meuth, Sven Pape, Hans‐Christian Budde, Thomas 0953-816X 1460-9568 Wiley General Neuroscience http://dx.doi.org/10.1046/j.1460-9568.2002.01999.x <jats:title>Abstract</jats:title><jats:p>Rhythmic low and high frequency activity in thalamocortical networks depend critically on activation of low‐ and high‐voltage‐activated (LVA, HVA) Ca<jats:sup>2+</jats:sup>currents. In order to test whether Ca<jats:sup>2+</jats:sup>currents are modified during repetitive activation, acutely isolated thalamocortical relay neurons of rats, at postnatal days 12 (P12) to P20, were investigated using patch‐clamp, Ca<jats:sup>2+</jats:sup>imaging and Western blot techniques. High‐voltage‐activated, but not LVA Ca<jats:sup>2+</jats:sup>currents were reduced significantly during 2 Hz stimulation. Ca<jats:sup>2+</jats:sup>imaging experiments demonstrated a close correlation between the increase in intracellular Ca<jats:sup>2+</jats:sup>levels and the decrease in HVA Ca<jats:sup>2+</jats:sup>current amplitudes. Further examination of HVA Ca<jats:sup>2+</jats:sup>currents revealed a ‘U‐shaped’ inactivation curve and a time‐dependent inactivation process that could be described by a two‐exponential function. The ‘U‐shape’ was significantly reduced, current amplitude was increased significantly and time‐dependent inactivation revealed a one‐exponential decline with Ba<jats:sup>2+</jats:sup>as the charge carrier, following activation of the cAMP/PKA pathway, and following application of phosphatase inhibitors (ascomycin, calyculin A). Western blot analysis and the effect of ascomycin indicated an involvement of calcineurin in the inactivation process. Isolation of HVA Ca<jats:sup>2+</jats:sup>current components by subtype‐specific blockers revealed that changes in time‐dependent inactivation, inactivation curve and current amplitude were carried mainly by L‐type and N‐type Ca<jats:sup>2+</jats:sup>currents. Furthermore, Ca<jats:sup>2+</jats:sup>‐dependent inactivation was operative during stimulation protocols mimicking tonic action potential firing. These data indicate a modulation of L‐ and N‐type Ca<jats:sup>2+</jats:sup>channels by phosphorylation, resulting jointly in an increased intracellular Ca<jats:sup>2+</jats:sup>influx during activity of the ascending brainstem system, the latter occurring during states of wakefulness.</jats:p> Modulation of Ca<sup>2+</sup>currents in rat thalamocortical relay neurons by activity and phosphorylation European Journal of Neuroscience |
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2002 |
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| title |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_unstemmed |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_full |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_fullStr |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_full_unstemmed |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_short |
Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_sort |
modulation of ca<sup>2+</sup>currents in rat thalamocortical relay neurons by activity and phosphorylation |
| topic |
General Neuroscience |
| url |
http://dx.doi.org/10.1046/j.1460-9568.2002.01999.x |
| publishDate |
2002 |
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1603-1614 |
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<jats:title>Abstract</jats:title><jats:p>Rhythmic low and high frequency activity in thalamocortical networks depend critically on activation of low‐ and high‐voltage‐activated (LVA, HVA) Ca<jats:sup>2+</jats:sup>currents. In order to test whether Ca<jats:sup>2+</jats:sup>currents are modified during repetitive activation, acutely isolated thalamocortical relay neurons of rats, at postnatal days 12 (P12) to P20, were investigated using patch‐clamp, Ca<jats:sup>2+</jats:sup>imaging and Western blot techniques. High‐voltage‐activated, but not LVA Ca<jats:sup>2+</jats:sup>currents were reduced significantly during 2 Hz stimulation. Ca<jats:sup>2+</jats:sup>imaging experiments demonstrated a close correlation between the increase in intracellular Ca<jats:sup>2+</jats:sup>levels and the decrease in HVA Ca<jats:sup>2+</jats:sup>current amplitudes. Further examination of HVA Ca<jats:sup>2+</jats:sup>currents revealed a ‘U‐shaped’ inactivation curve and a time‐dependent inactivation process that could be described by a two‐exponential function. The ‘U‐shape’ was significantly reduced, current amplitude was increased significantly and time‐dependent inactivation revealed a one‐exponential decline with Ba<jats:sup>2+</jats:sup>as the charge carrier, following activation of the cAMP/PKA pathway, and following application of phosphatase inhibitors (ascomycin, calyculin A). Western blot analysis and the effect of ascomycin indicated an involvement of calcineurin in the inactivation process. Isolation of HVA Ca<jats:sup>2+</jats:sup>current components by subtype‐specific blockers revealed that changes in time‐dependent inactivation, inactivation curve and current amplitude were carried mainly by L‐type and N‐type Ca<jats:sup>2+</jats:sup>currents. Furthermore, Ca<jats:sup>2+</jats:sup>‐dependent inactivation was operative during stimulation protocols mimicking tonic action potential firing. These data indicate a modulation of L‐ and N‐type Ca<jats:sup>2+</jats:sup>channels by phosphorylation, resulting jointly in an increased intracellular Ca<jats:sup>2+</jats:sup>influx during activity of the ascending brainstem system, the latter occurring during states of wakefulness.</jats:p> |
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| author | Meuth, Sven, Pape, Hans‐Christian, Budde, Thomas |
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| description | <jats:title>Abstract</jats:title><jats:p>Rhythmic low and high frequency activity in thalamocortical networks depend critically on activation of low‐ and high‐voltage‐activated (LVA, HVA) Ca<jats:sup>2+</jats:sup>currents. In order to test whether Ca<jats:sup>2+</jats:sup>currents are modified during repetitive activation, acutely isolated thalamocortical relay neurons of rats, at postnatal days 12 (P12) to P20, were investigated using patch‐clamp, Ca<jats:sup>2+</jats:sup>imaging and Western blot techniques. High‐voltage‐activated, but not LVA Ca<jats:sup>2+</jats:sup>currents were reduced significantly during 2 Hz stimulation. Ca<jats:sup>2+</jats:sup>imaging experiments demonstrated a close correlation between the increase in intracellular Ca<jats:sup>2+</jats:sup>levels and the decrease in HVA Ca<jats:sup>2+</jats:sup>current amplitudes. Further examination of HVA Ca<jats:sup>2+</jats:sup>currents revealed a ‘U‐shaped’ inactivation curve and a time‐dependent inactivation process that could be described by a two‐exponential function. The ‘U‐shape’ was significantly reduced, current amplitude was increased significantly and time‐dependent inactivation revealed a one‐exponential decline with Ba<jats:sup>2+</jats:sup>as the charge carrier, following activation of the cAMP/PKA pathway, and following application of phosphatase inhibitors (ascomycin, calyculin A). Western blot analysis and the effect of ascomycin indicated an involvement of calcineurin in the inactivation process. Isolation of HVA Ca<jats:sup>2+</jats:sup>current components by subtype‐specific blockers revealed that changes in time‐dependent inactivation, inactivation curve and current amplitude were carried mainly by L‐type and N‐type Ca<jats:sup>2+</jats:sup>currents. Furthermore, Ca<jats:sup>2+</jats:sup>‐dependent inactivation was operative during stimulation protocols mimicking tonic action potential firing. These data indicate a modulation of L‐ and N‐type Ca<jats:sup>2+</jats:sup>channels by phosphorylation, resulting jointly in an increased intracellular Ca<jats:sup>2+</jats:sup>influx during activity of the ascending brainstem system, the latter occurring during states of wakefulness.</jats:p> |
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| imprint | Wiley, 2002 |
| imprint_str_mv | Wiley, 2002 |
| institution | DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
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| series | European Journal of Neuroscience |
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| spelling | Meuth, Sven Pape, Hans‐Christian Budde, Thomas 0953-816X 1460-9568 Wiley General Neuroscience http://dx.doi.org/10.1046/j.1460-9568.2002.01999.x <jats:title>Abstract</jats:title><jats:p>Rhythmic low and high frequency activity in thalamocortical networks depend critically on activation of low‐ and high‐voltage‐activated (LVA, HVA) Ca<jats:sup>2+</jats:sup>currents. In order to test whether Ca<jats:sup>2+</jats:sup>currents are modified during repetitive activation, acutely isolated thalamocortical relay neurons of rats, at postnatal days 12 (P12) to P20, were investigated using patch‐clamp, Ca<jats:sup>2+</jats:sup>imaging and Western blot techniques. High‐voltage‐activated, but not LVA Ca<jats:sup>2+</jats:sup>currents were reduced significantly during 2 Hz stimulation. Ca<jats:sup>2+</jats:sup>imaging experiments demonstrated a close correlation between the increase in intracellular Ca<jats:sup>2+</jats:sup>levels and the decrease in HVA Ca<jats:sup>2+</jats:sup>current amplitudes. Further examination of HVA Ca<jats:sup>2+</jats:sup>currents revealed a ‘U‐shaped’ inactivation curve and a time‐dependent inactivation process that could be described by a two‐exponential function. The ‘U‐shape’ was significantly reduced, current amplitude was increased significantly and time‐dependent inactivation revealed a one‐exponential decline with Ba<jats:sup>2+</jats:sup>as the charge carrier, following activation of the cAMP/PKA pathway, and following application of phosphatase inhibitors (ascomycin, calyculin A). Western blot analysis and the effect of ascomycin indicated an involvement of calcineurin in the inactivation process. Isolation of HVA Ca<jats:sup>2+</jats:sup>current components by subtype‐specific blockers revealed that changes in time‐dependent inactivation, inactivation curve and current amplitude were carried mainly by L‐type and N‐type Ca<jats:sup>2+</jats:sup>currents. Furthermore, Ca<jats:sup>2+</jats:sup>‐dependent inactivation was operative during stimulation protocols mimicking tonic action potential firing. These data indicate a modulation of L‐ and N‐type Ca<jats:sup>2+</jats:sup>channels by phosphorylation, resulting jointly in an increased intracellular Ca<jats:sup>2+</jats:sup>influx during activity of the ascending brainstem system, the latter occurring during states of wakefulness.</jats:p> Modulation of Ca<sup>2+</sup>currents in rat thalamocortical relay neurons by activity and phosphorylation European Journal of Neuroscience |
| spellingShingle | Meuth, Sven, Pape, Hans‐Christian, Budde, Thomas, European Journal of Neuroscience, Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation, General Neuroscience |
| title | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_full | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_fullStr | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_full_unstemmed | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_short | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_sort | modulation of ca<sup>2+</sup>currents in rat thalamocortical relay neurons by activity and phosphorylation |
| title_unstemmed | Modulation of Ca2+currents in rat thalamocortical relay neurons by activity and phosphorylation |
| topic | General Neuroscience |
| url | http://dx.doi.org/10.1046/j.1460-9568.2002.01999.x |