Eintrag weiter verarbeiten
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells
Gespeichert in:
| Zeitschriftentitel: | Bioscience Reports |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Bioscience Reports, 40, 2020, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
|
| Schlagwörter: |
| author_facet |
Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang |
|---|---|
| author |
Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang |
| spellingShingle |
Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang Bioscience Reports RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells Cell Biology Molecular Biology Biochemistry Biophysics |
| author_sort |
chen, hong-min |
| spelling |
Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20192759 <jats:title>Abstract</jats:title> <jats:p>High glucose (HG)-induced mitochondrial dynamic changes and oxidative damage are closely related to the development and progression of diabetic kidney disease (DKD). Recent studies suggest that regulators of calcineurin 1 (RCAN1) is involved in the regulation of mitochondrial function in different cell types, so we investigate the role of RCAN1 in mitochondrial dynamics under HG ambience in rat glomerular mesangial cells (MCs). MCs subjected to HG exhibited an isoform-specific up-regulation of RCAN1.4 at both mRNA and protein levels. RCAN1.4 overexpression induced translocation of Dynamin related protein 1 (Drp1) to mitochondria, mitochondrial fragmentation and depolarization, accompanied by increased matrix production under normal glucose and HG ambience. In contrast, decreasing the expression of RCAN1.4 by siRNA inhibited HG-induced mitochondrial fragmentation and matrix protein up-regulation. Moreover, both mitochondrial fission inhibitor Mdivi-1 and Drp1 shRNA prevented RCAN1.4-induced fibronectin up-regulation, suggesting that RCAN1.4-induced matrix production is dependent on its modulation of mitochondrial fission. Although HG-induced RCAN1.4 up-regulation was achieved by activating calcineurin, RCAN1.4-mediated mitochondrial fragmentation and matrix production is independent of calcineurin activity. These results provide the first evidence for the HG-induced RCAN1.4 up-regulation involving increased mitochondrial fragmentation, leading to matrix protein up-regulation.</jats:p> RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells Bioscience Reports |
| doi_str_mv |
10.1042/bsr20192759 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Chemie und Pharmazie Physik |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE5Mjc1OQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE5Mjc1OQ |
| institution |
DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
Portland Press Ltd., 2020 |
| imprint_str_mv |
Portland Press Ltd., 2020 |
| issn |
0144-8463 1573-4935 |
| issn_str_mv |
0144-8463 1573-4935 |
| language |
English |
| mega_collection |
Portland Press Ltd. (CrossRef) |
| match_str |
chen2020rcan14mediateshighglucoseinducedmatrixproductionbystimulatingmitochondrialfissioninmesangialcells |
| publishDateSort |
2020 |
| publisher |
Portland Press Ltd. |
| recordtype |
ai |
| record_format |
ai |
| series |
Bioscience Reports |
| source_id |
49 |
| title |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_unstemmed |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_full |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_fullStr |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_full_unstemmed |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_short |
RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_sort |
rcan1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| topic |
Cell Biology Molecular Biology Biochemistry Biophysics |
| url |
http://dx.doi.org/10.1042/bsr20192759 |
| publishDate |
2020 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:p>High glucose (HG)-induced mitochondrial dynamic changes and oxidative damage are closely related to the development and progression of diabetic kidney disease (DKD). Recent studies suggest that regulators of calcineurin 1 (RCAN1) is involved in the regulation of mitochondrial function in different cell types, so we investigate the role of RCAN1 in mitochondrial dynamics under HG ambience in rat glomerular mesangial cells (MCs). MCs subjected to HG exhibited an isoform-specific up-regulation of RCAN1.4 at both mRNA and protein levels. RCAN1.4 overexpression induced translocation of Dynamin related protein 1 (Drp1) to mitochondria, mitochondrial fragmentation and depolarization, accompanied by increased matrix production under normal glucose and HG ambience. In contrast, decreasing the expression of RCAN1.4 by siRNA inhibited HG-induced mitochondrial fragmentation and matrix protein up-regulation. Moreover, both mitochondrial fission inhibitor Mdivi-1 and Drp1 shRNA prevented RCAN1.4-induced fibronectin up-regulation, suggesting that RCAN1.4-induced matrix production is dependent on its modulation of mitochondrial fission. Although HG-induced RCAN1.4 up-regulation was achieved by activating calcineurin, RCAN1.4-mediated mitochondrial fragmentation and matrix production is independent of calcineurin activity. These results provide the first evidence for the HG-induced RCAN1.4 up-regulation involving increased mitochondrial fragmentation, leading to matrix protein up-regulation.</jats:p> |
| container_issue |
1 |
| container_start_page |
0 |
| container_title |
Bioscience Reports |
| container_volume |
40 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792341188605378566 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:15:57.146Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=RCAN1.4+mediates+high+glucose-induced+matrix+production+by+stimulating+mitochondrial+fission+in+mesangial+cells&rft.date=2020-01-31&genre=article&issn=1573-4935&volume=40&issue=1&jtitle=Bioscience+Reports&atitle=RCAN1.4+mediates+high+glucose-induced+matrix+production+by+stimulating+mitochondrial+fission+in+mesangial+cells&aulast=Zhang&aufirst=Bai-Fang&rft_id=info%3Adoi%2F10.1042%2Fbsr20192759&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792341188605378566 |
| author | Chen, Hong-Min, Dai, Jia-Jia, Zhu, Rui, Sang, Xue-Yu, Peng, Fang-Fang, Yu, Hong, Zhang, Bai-Fang |
| author_facet | Chen, Hong-Min, Dai, Jia-Jia, Zhu, Rui, Sang, Xue-Yu, Peng, Fang-Fang, Yu, Hong, Zhang, Bai-Fang, Chen, Hong-Min, Dai, Jia-Jia, Zhu, Rui, Sang, Xue-Yu, Peng, Fang-Fang, Yu, Hong, Zhang, Bai-Fang |
| author_sort | chen, hong-min |
| container_issue | 1 |
| container_start_page | 0 |
| container_title | Bioscience Reports |
| container_volume | 40 |
| description | <jats:title>Abstract</jats:title> <jats:p>High glucose (HG)-induced mitochondrial dynamic changes and oxidative damage are closely related to the development and progression of diabetic kidney disease (DKD). Recent studies suggest that regulators of calcineurin 1 (RCAN1) is involved in the regulation of mitochondrial function in different cell types, so we investigate the role of RCAN1 in mitochondrial dynamics under HG ambience in rat glomerular mesangial cells (MCs). MCs subjected to HG exhibited an isoform-specific up-regulation of RCAN1.4 at both mRNA and protein levels. RCAN1.4 overexpression induced translocation of Dynamin related protein 1 (Drp1) to mitochondria, mitochondrial fragmentation and depolarization, accompanied by increased matrix production under normal glucose and HG ambience. In contrast, decreasing the expression of RCAN1.4 by siRNA inhibited HG-induced mitochondrial fragmentation and matrix protein up-regulation. Moreover, both mitochondrial fission inhibitor Mdivi-1 and Drp1 shRNA prevented RCAN1.4-induced fibronectin up-regulation, suggesting that RCAN1.4-induced matrix production is dependent on its modulation of mitochondrial fission. Although HG-induced RCAN1.4 up-regulation was achieved by activating calcineurin, RCAN1.4-mediated mitochondrial fragmentation and matrix production is independent of calcineurin activity. These results provide the first evidence for the HG-induced RCAN1.4 up-regulation involving increased mitochondrial fragmentation, leading to matrix protein up-regulation.</jats:p> |
| doi_str_mv | 10.1042/bsr20192759 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Chemie und Pharmazie, Physik |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE5Mjc1OQ |
| imprint | Portland Press Ltd., 2020 |
| imprint_str_mv | Portland Press Ltd., 2020 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 0144-8463, 1573-4935 |
| issn_str_mv | 0144-8463, 1573-4935 |
| language | English |
| last_indexed | 2024-03-01T16:15:57.146Z |
| match_str | chen2020rcan14mediateshighglucoseinducedmatrixproductionbystimulatingmitochondrialfissioninmesangialcells |
| mega_collection | Portland Press Ltd. (CrossRef) |
| physical | |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Portland Press Ltd. |
| record_format | ai |
| recordtype | ai |
| series | Bioscience Reports |
| source_id | 49 |
| spelling | Chen, Hong-Min Dai, Jia-Jia Zhu, Rui Sang, Xue-Yu Peng, Fang-Fang Yu, Hong Zhang, Bai-Fang 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20192759 <jats:title>Abstract</jats:title> <jats:p>High glucose (HG)-induced mitochondrial dynamic changes and oxidative damage are closely related to the development and progression of diabetic kidney disease (DKD). Recent studies suggest that regulators of calcineurin 1 (RCAN1) is involved in the regulation of mitochondrial function in different cell types, so we investigate the role of RCAN1 in mitochondrial dynamics under HG ambience in rat glomerular mesangial cells (MCs). MCs subjected to HG exhibited an isoform-specific up-regulation of RCAN1.4 at both mRNA and protein levels. RCAN1.4 overexpression induced translocation of Dynamin related protein 1 (Drp1) to mitochondria, mitochondrial fragmentation and depolarization, accompanied by increased matrix production under normal glucose and HG ambience. In contrast, decreasing the expression of RCAN1.4 by siRNA inhibited HG-induced mitochondrial fragmentation and matrix protein up-regulation. Moreover, both mitochondrial fission inhibitor Mdivi-1 and Drp1 shRNA prevented RCAN1.4-induced fibronectin up-regulation, suggesting that RCAN1.4-induced matrix production is dependent on its modulation of mitochondrial fission. Although HG-induced RCAN1.4 up-regulation was achieved by activating calcineurin, RCAN1.4-mediated mitochondrial fragmentation and matrix production is independent of calcineurin activity. These results provide the first evidence for the HG-induced RCAN1.4 up-regulation involving increased mitochondrial fragmentation, leading to matrix protein up-regulation.</jats:p> RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells Bioscience Reports |
| spellingShingle | Chen, Hong-Min, Dai, Jia-Jia, Zhu, Rui, Sang, Xue-Yu, Peng, Fang-Fang, Yu, Hong, Zhang, Bai-Fang, Bioscience Reports, RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells, Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| title | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_full | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_fullStr | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_full_unstemmed | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_short | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_sort | rcan1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| title_unstemmed | RCAN1.4 mediates high glucose-induced matrix production by stimulating mitochondrial fission in mesangial cells |
| topic | Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| url | http://dx.doi.org/10.1042/bsr20192759 |