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Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410
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| Zeitschriftentitel: | Bioscience Reports |
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| Personen und Körperschaften: | , , , , , , , |
| In: | Bioscience Reports, 39, 2019, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
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| Schlagwörter: |
| author_facet |
Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua |
|---|---|
| author |
Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua |
| spellingShingle |
Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua Bioscience Reports Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 Cell Biology Molecular Biology Biochemistry Biophysics |
| author_sort |
sun, wei-li |
| spelling |
Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20180395 <jats:title>Abstract</jats:title> <jats:p>The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. In vivo, OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.</jats:p> Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 Bioscience Reports |
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| title |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_unstemmed |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_full |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_fullStr |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_full_unstemmed |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_short |
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_sort |
long noncoding rna oip5-as1 targets wnt-7b to affect glioma progression via modulation of mir-410 |
| topic |
Cell Biology Molecular Biology Biochemistry Biophysics |
| url |
http://dx.doi.org/10.1042/bsr20180395 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:p>The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. In vivo, OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.</jats:p> |
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| author_facet | Sun, Wei-Li, Kang, Tian, Wang, Yuan-Yu, Sun, Jian-Ping, Li, Chen, Liu, Hong-Jiang, Yang, Yue, Jiao, Bao-Hua, Sun, Wei-Li, Kang, Tian, Wang, Yuan-Yu, Sun, Jian-Ping, Li, Chen, Liu, Hong-Jiang, Yang, Yue, Jiao, Bao-Hua |
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| description | <jats:title>Abstract</jats:title> <jats:p>The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. In vivo, OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.</jats:p> |
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| institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
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| spelling | Sun, Wei-Li Kang, Tian Wang, Yuan-Yu Sun, Jian-Ping Li, Chen Liu, Hong-Jiang Yang, Yue Jiao, Bao-Hua 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20180395 <jats:title>Abstract</jats:title> <jats:p>The present study was undertaken to investigate the underlying mechanisms of long noncoding RNA OIP5-AS1 via regulating miR-410 to modulate Wnt-7b in the progression of glioma. To address this problem, we measured the expression of OIP5-AS1 and miR-410 in glioma tissues by qRT-PCR. Glioma U87 cells were transfected with OIP5-AS1 siRNA or miR-410 inhibitors. The targeting relationships among miR-410, OIP5-AS1 and Wnt-7b were verified by luciferase reporter assays. Western blotting was employed to determine the expression of Wnt-7b/β-catenin pathway-related proteins, while MTT, flow cytometry, Transwell assays and wound-healing assays were used to measure the biological characteristics of glioma cells. The results showed that OIP5-AS1 expression was higher and miR-410 was lower in glioma tissues. Luciferase reporter assays confirmed a targeting relationship between OIP5-AS1 and miR-410, as well as between miR-410 and Wnt-7b. Silencing OIP5-AS1 reduced cell proliferation, invasion and migration of glioma U87 cells and led to depressed expression levels of miR-410, Wnt-7b, p-β-catenin, GSK-3β-pS9, c-Myc and cyclin D1. Furthermore, down-regulation of OIP5-AS1 induced G0/G1 phase cell cycle arrest and apoptosis of glioma cells. Inhibitors of miR-410 abolished the biological effects of OIP5-AS1 siRNA in glioma cells. In vivo, OIP5-AS1 knockdown also inhibited tumor growth. Taken together, this research suggested that silencing OIP5-AS1 may specifically block the Wnt-7b/β-catenin pathway via targeted up-regulating miR-410, thereby inhibiting growth, invasion and migration while promoting apoptosis in glioma cells.</jats:p> Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 Bioscience Reports |
| spellingShingle | Sun, Wei-Li, Kang, Tian, Wang, Yuan-Yu, Sun, Jian-Ping, Li, Chen, Liu, Hong-Jiang, Yang, Yue, Jiao, Bao-Hua, Bioscience Reports, Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410, Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| title | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_full | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_fullStr | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_full_unstemmed | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_short | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| title_sort | long noncoding rna oip5-as1 targets wnt-7b to affect glioma progression via modulation of mir-410 |
| title_unstemmed | Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410 |
| topic | Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| url | http://dx.doi.org/10.1042/bsr20180395 |