Eintrag weiter verarbeiten
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo
Gespeichert in:
| Zeitschriftentitel: | Bioscience Reports |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Bioscience Reports, 36, 2016, 3 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
|
| Schlagwörter: |
| author_facet |
Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing |
|---|---|
| author |
Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing |
| spellingShingle |
Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing Bioscience Reports LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo Cell Biology Molecular Biology Biochemistry Biophysics |
| author_sort |
sun, longci |
| spelling |
Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20160048 <jats:p>Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo. Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro. Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression.</jats:p> LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both <i>in vitro</i> and <i>in vivo</i> Bioscience Reports |
| doi_str_mv |
10.1042/bsr20160048 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Chemie und Pharmazie Physik |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE2MDA0OA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE2MDA0OA |
| institution |
DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
| imprint |
Portland Press Ltd., 2016 |
| imprint_str_mv |
Portland Press Ltd., 2016 |
| issn |
0144-8463 1573-4935 |
| issn_str_mv |
0144-8463 1573-4935 |
| language |
English |
| mega_collection |
Portland Press Ltd. (CrossRef) |
| match_str |
sun2016lncrnadq786243contributestoproliferationandmetastasisofcolorectalcancerbothinvitroandinvivo |
| publishDateSort |
2016 |
| publisher |
Portland Press Ltd. |
| recordtype |
ai |
| record_format |
ai |
| series |
Bioscience Reports |
| source_id |
49 |
| title |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_unstemmed |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_full |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_fullStr |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_full_unstemmed |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_short |
LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_sort |
lncrna dq786243 contributes to proliferation and metastasis of colorectal cancer both <i>in vitro</i> and <i>in vivo</i> |
| topic |
Cell Biology Molecular Biology Biochemistry Biophysics |
| url |
http://dx.doi.org/10.1042/bsr20160048 |
| publishDate |
2016 |
| physical |
|
| description |
<jats:p>Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo. Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro. Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression.</jats:p> |
| container_issue |
3 |
| container_start_page |
0 |
| container_title |
Bioscience Reports |
| container_volume |
36 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336363801018376 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:58:54.68Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=LncRNA+DQ786243+contributes+to+proliferation+and+metastasis+of+colorectal+cancer+both+in%C2%A0vitro+and+in%C2%A0vivo&rft.date=2016-06-01&genre=article&issn=1573-4935&volume=36&issue=3&jtitle=Bioscience+Reports&atitle=LncRNA+DQ786243+contributes+to+proliferation+and+metastasis+of+colorectal+cancer+both+%3Ci%3Ein%C2%A0vitro%3C%2Fi%3E+and+%3Ci%3Ein%C2%A0vivo%3C%2Fi%3E&aulast=Xu&aufirst=Qing&rft_id=info%3Adoi%2F10.1042%2Fbsr20160048&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336363801018376 |
| author | Sun, Longci, Xue, Hanbing, Jiang, Chunhui, Zhou, Hong, Gu, Lei, Liu, Ye, Xu, Chunjie, Xu, Qing |
| author_facet | Sun, Longci, Xue, Hanbing, Jiang, Chunhui, Zhou, Hong, Gu, Lei, Liu, Ye, Xu, Chunjie, Xu, Qing, Sun, Longci, Xue, Hanbing, Jiang, Chunhui, Zhou, Hong, Gu, Lei, Liu, Ye, Xu, Chunjie, Xu, Qing |
| author_sort | sun, longci |
| container_issue | 3 |
| container_start_page | 0 |
| container_title | Bioscience Reports |
| container_volume | 36 |
| description | <jats:p>Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo. Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro. Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression.</jats:p> |
| doi_str_mv | 10.1042/bsr20160048 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Chemie und Pharmazie, Physik |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9ic3IyMDE2MDA0OA |
| imprint | Portland Press Ltd., 2016 |
| imprint_str_mv | Portland Press Ltd., 2016 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
| issn | 0144-8463, 1573-4935 |
| issn_str_mv | 0144-8463, 1573-4935 |
| language | English |
| last_indexed | 2024-03-01T14:58:54.68Z |
| match_str | sun2016lncrnadq786243contributestoproliferationandmetastasisofcolorectalcancerbothinvitroandinvivo |
| mega_collection | Portland Press Ltd. (CrossRef) |
| physical | |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Portland Press Ltd. |
| record_format | ai |
| recordtype | ai |
| series | Bioscience Reports |
| source_id | 49 |
| spelling | Sun, Longci Xue, Hanbing Jiang, Chunhui Zhou, Hong Gu, Lei Liu, Ye Xu, Chunjie Xu, Qing 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20160048 <jats:p>Accumulating evidence demonstrates that long non-coding RNAs (LncRNAs) play important roles in regulating gene expression and are involved in various cancers, including colorectal cancer (CRC). However, LncRNA profiles in CRC remain largely unknown. The present study aims to find the key LncRNA associated with CRC and to study its biological functions in CRC progression. We focused on LncRNA DQ786243, one of LncRNAs which promoted development of CRC from the Gene Expression Omnibus (GEO) and validated using quantitative real-time PCR among about 20 paired CRC tissues. The effects of LncRNA DQ786243 were assessed by silencing the LncRNA in vitro and in vivo. Results showed that the expression level LncRNA DQ786243 was significantly higher in CRC tissues and cell lines. We also found LncRNA DQ786243 knockdown by RNA interference with siRNA significantly arrested the cell cycle in the G2/M-phase, promoted apoptosis and weaken the abilities of cell proliferation and invasion in vitro. Further investigation into the mechanisms responsible for the growth inhibitory effects by DQ786243 silencing revealed that its knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. Finally, xenograft experiments confirmed that the growth of xenograft tumours formed by CRC cells was suppressed after silencing LncRNA DQ786243 expression. In conclusion, the present study suggests that LncRNA DQ786243 is an oncogene that promotes tumour progression and leads us to propose that LncRNAs may serve as key regulatory hubs in CRC progression.</jats:p> LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both <i>in vitro</i> and <i>in vivo</i> Bioscience Reports |
| spellingShingle | Sun, Longci, Xue, Hanbing, Jiang, Chunhui, Zhou, Hong, Gu, Lei, Liu, Ye, Xu, Chunjie, Xu, Qing, Bioscience Reports, LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo, Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| title | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_full | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_fullStr | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_full_unstemmed | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_short | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| title_sort | lncrna dq786243 contributes to proliferation and metastasis of colorectal cancer both <i>in vitro</i> and <i>in vivo</i> |
| title_unstemmed | LncRNA DQ786243 contributes to proliferation and metastasis of colorectal cancer both in vitro and in vivo |
| topic | Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| url | http://dx.doi.org/10.1042/bsr20160048 |