Eintrag weiter verarbeiten
The inositol phosphates in WRK1 rat mammary tumour cells
Gespeichert in:
Zeitschriftentitel: | Biochemical Journal |
---|---|
Personen und Körperschaften: | , , , , , |
In: | Biochemical Journal, 286, 1992, 2, S. 459-468 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Portland Press Ltd.
|
Schlagwörter: |
author_facet |
Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H |
---|---|
author |
Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H |
spellingShingle |
Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H Biochemical Journal The inositol phosphates in WRK1 rat mammary tumour cells Cell Biology Molecular Biology Biochemistry |
author_sort |
wong, n s |
spelling |
Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H 0264-6021 1470-8728 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj2860459 <jats:p>1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated cells included an uncharacterized peak, probably containing some glycerophosphoinositol, and Ins(1:2-cyclic)P. Stimulation provoked accumulation of both Ins1P and Ins3P, of Ins2P, and of Ins5P and/or the enantiomers Ins4P and Ins6P. The proportions of Ins1P and Ins3P were determined by partial periodate oxidation and enantiomeric identification of the resulting glucitols. 3. Three inositol bisphosphate peaks were detected in unstimulated cells: Ins(1,4)P2 [this was distinguished chemically from its enantiomer Ins(3,6)P2], Ins(3,4)P2 and/or Ins(1,6)P2, and Ins(4,5)P2 and/or Ins(5,6)P2. On stimulation, Ins(1,4)P2 and Ins(3,4)P2 [and/or Ins(1,6)P2] levels increased, and Ins(1:2-cyclic,4)P2 and Ins(1,3)P2 were also formed. 4. Three inositol trisphosphate peaks were obtained from unstimulated cells: all increased during stimulation. These were Ins(1,3,4)P3 [with some Ins(1:2-cyclic,4,5)P3], Ins(1,4,5)P3 and Ins(3,4,5)P3 [and/or Ins(1,5,6)P3]. During stimulation, another compound, probably Ins(1,4,6)P3, appeared in the ‘Ins(1,4,5)P3 peak’. The ‘Ins(3,4,5)P3 peak’ contained a second trisphosphate, probably Ins(2,4,5)P3. 5. Three inositol tetrakisphosphates, namely Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, were present in unstimulated cells, and all accumulated during stimulation. 6. Ins(1,3,4,5,6)P5, which is the most abundant inositol polyphosphate in these cells, a less abundant inositol pentakisphosphate and inositol hexakisphosphate were all unresponsive to stimulation.</jats:p> The inositol phosphates in WRK1 rat mammary tumour cells Biochemical Journal |
doi_str_mv |
10.1042/bj2860459 |
facet_avail |
Online Free |
finc_class_facet |
Biologie Chemie und Pharmazie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajI4NjA0NTk |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajI4NjA0NTk |
institution |
DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
imprint |
Portland Press Ltd., 1992 |
imprint_str_mv |
Portland Press Ltd., 1992 |
issn |
0264-6021 1470-8728 |
issn_str_mv |
0264-6021 1470-8728 |
language |
English |
mega_collection |
Portland Press Ltd. (CrossRef) |
match_str |
wong1992theinositolphosphatesinwrk1ratmammarytumourcells |
publishDateSort |
1992 |
publisher |
Portland Press Ltd. |
recordtype |
ai |
record_format |
ai |
series |
Biochemical Journal |
source_id |
49 |
title |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_unstemmed |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_full |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_fullStr |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_full_unstemmed |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_short |
The inositol phosphates in WRK1 rat mammary tumour cells |
title_sort |
the inositol phosphates in wrk1 rat mammary tumour cells |
topic |
Cell Biology Molecular Biology Biochemistry |
url |
http://dx.doi.org/10.1042/bj2860459 |
publishDate |
1992 |
physical |
459-468 |
description |
<jats:p>1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated cells included an uncharacterized peak, probably containing some glycerophosphoinositol, and Ins(1:2-cyclic)P. Stimulation provoked accumulation of both Ins1P and Ins3P, of Ins2P, and of Ins5P and/or the enantiomers Ins4P and Ins6P. The proportions of Ins1P and Ins3P were determined by partial periodate oxidation and enantiomeric identification of the resulting glucitols. 3. Three inositol bisphosphate peaks were detected in unstimulated cells: Ins(1,4)P2 [this was distinguished chemically from its enantiomer Ins(3,6)P2], Ins(3,4)P2 and/or Ins(1,6)P2, and Ins(4,5)P2 and/or Ins(5,6)P2. On stimulation, Ins(1,4)P2 and Ins(3,4)P2 [and/or Ins(1,6)P2] levels increased, and Ins(1:2-cyclic,4)P2 and Ins(1,3)P2 were also formed. 4. Three inositol trisphosphate peaks were obtained from unstimulated cells: all increased during stimulation. These were Ins(1,3,4)P3 [with some Ins(1:2-cyclic,4,5)P3], Ins(1,4,5)P3 and Ins(3,4,5)P3 [and/or Ins(1,5,6)P3]. During stimulation, another compound, probably Ins(1,4,6)P3, appeared in the ‘Ins(1,4,5)P3 peak’. The ‘Ins(3,4,5)P3 peak’ contained a second trisphosphate, probably Ins(2,4,5)P3. 5. Three inositol tetrakisphosphates, namely Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, were present in unstimulated cells, and all accumulated during stimulation. 6. Ins(1,3,4,5,6)P5, which is the most abundant inositol polyphosphate in these cells, a less abundant inositol pentakisphosphate and inositol hexakisphosphate were all unresponsive to stimulation.</jats:p> |
container_issue |
2 |
container_start_page |
459 |
container_title |
Biochemical Journal |
container_volume |
286 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792342906556645384 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T16:43:06.417Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=The+inositol+phosphates+in+WRK1+rat+mammary+tumour+cells&rft.date=1992-09-01&genre=article&issn=1470-8728&volume=286&issue=2&spage=459&epage=468&pages=459-468&jtitle=Biochemical+Journal&atitle=The+inositol+phosphates+in+WRK1+rat+mammary+tumour+cells&aulast=Michell&aufirst=R+H&rft_id=info%3Adoi%2F10.1042%2Fbj2860459&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792342906556645384 |
author | Wong, N S, Barker, C J, Morris, A J, Craxton, A, Kirk, C J, Michell, R H |
author_facet | Wong, N S, Barker, C J, Morris, A J, Craxton, A, Kirk, C J, Michell, R H, Wong, N S, Barker, C J, Morris, A J, Craxton, A, Kirk, C J, Michell, R H |
author_sort | wong, n s |
container_issue | 2 |
container_start_page | 459 |
container_title | Biochemical Journal |
container_volume | 286 |
description | <jats:p>1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated cells included an uncharacterized peak, probably containing some glycerophosphoinositol, and Ins(1:2-cyclic)P. Stimulation provoked accumulation of both Ins1P and Ins3P, of Ins2P, and of Ins5P and/or the enantiomers Ins4P and Ins6P. The proportions of Ins1P and Ins3P were determined by partial periodate oxidation and enantiomeric identification of the resulting glucitols. 3. Three inositol bisphosphate peaks were detected in unstimulated cells: Ins(1,4)P2 [this was distinguished chemically from its enantiomer Ins(3,6)P2], Ins(3,4)P2 and/or Ins(1,6)P2, and Ins(4,5)P2 and/or Ins(5,6)P2. On stimulation, Ins(1,4)P2 and Ins(3,4)P2 [and/or Ins(1,6)P2] levels increased, and Ins(1:2-cyclic,4)P2 and Ins(1,3)P2 were also formed. 4. Three inositol trisphosphate peaks were obtained from unstimulated cells: all increased during stimulation. These were Ins(1,3,4)P3 [with some Ins(1:2-cyclic,4,5)P3], Ins(1,4,5)P3 and Ins(3,4,5)P3 [and/or Ins(1,5,6)P3]. During stimulation, another compound, probably Ins(1,4,6)P3, appeared in the ‘Ins(1,4,5)P3 peak’. The ‘Ins(3,4,5)P3 peak’ contained a second trisphosphate, probably Ins(2,4,5)P3. 5. Three inositol tetrakisphosphates, namely Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, were present in unstimulated cells, and all accumulated during stimulation. 6. Ins(1,3,4,5,6)P5, which is the most abundant inositol polyphosphate in these cells, a less abundant inositol pentakisphosphate and inositol hexakisphosphate were all unresponsive to stimulation.</jats:p> |
doi_str_mv | 10.1042/bj2860459 |
facet_avail | Online, Free |
finc_class_facet | Biologie, Chemie und Pharmazie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajI4NjA0NTk |
imprint | Portland Press Ltd., 1992 |
imprint_str_mv | Portland Press Ltd., 1992 |
institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
issn | 0264-6021, 1470-8728 |
issn_str_mv | 0264-6021, 1470-8728 |
language | English |
last_indexed | 2024-03-01T16:43:06.417Z |
match_str | wong1992theinositolphosphatesinwrk1ratmammarytumourcells |
mega_collection | Portland Press Ltd. (CrossRef) |
physical | 459-468 |
publishDate | 1992 |
publishDateSort | 1992 |
publisher | Portland Press Ltd. |
record_format | ai |
recordtype | ai |
series | Biochemical Journal |
source_id | 49 |
spelling | Wong, N S Barker, C J Morris, A J Craxton, A Kirk, C J Michell, R H 0264-6021 1470-8728 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj2860459 <jats:p>1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated cells included an uncharacterized peak, probably containing some glycerophosphoinositol, and Ins(1:2-cyclic)P. Stimulation provoked accumulation of both Ins1P and Ins3P, of Ins2P, and of Ins5P and/or the enantiomers Ins4P and Ins6P. The proportions of Ins1P and Ins3P were determined by partial periodate oxidation and enantiomeric identification of the resulting glucitols. 3. Three inositol bisphosphate peaks were detected in unstimulated cells: Ins(1,4)P2 [this was distinguished chemically from its enantiomer Ins(3,6)P2], Ins(3,4)P2 and/or Ins(1,6)P2, and Ins(4,5)P2 and/or Ins(5,6)P2. On stimulation, Ins(1,4)P2 and Ins(3,4)P2 [and/or Ins(1,6)P2] levels increased, and Ins(1:2-cyclic,4)P2 and Ins(1,3)P2 were also formed. 4. Three inositol trisphosphate peaks were obtained from unstimulated cells: all increased during stimulation. These were Ins(1,3,4)P3 [with some Ins(1:2-cyclic,4,5)P3], Ins(1,4,5)P3 and Ins(3,4,5)P3 [and/or Ins(1,5,6)P3]. During stimulation, another compound, probably Ins(1,4,6)P3, appeared in the ‘Ins(1,4,5)P3 peak’. The ‘Ins(3,4,5)P3 peak’ contained a second trisphosphate, probably Ins(2,4,5)P3. 5. Three inositol tetrakisphosphates, namely Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, were present in unstimulated cells, and all accumulated during stimulation. 6. Ins(1,3,4,5,6)P5, which is the most abundant inositol polyphosphate in these cells, a less abundant inositol pentakisphosphate and inositol hexakisphosphate were all unresponsive to stimulation.</jats:p> The inositol phosphates in WRK1 rat mammary tumour cells Biochemical Journal |
spellingShingle | Wong, N S, Barker, C J, Morris, A J, Craxton, A, Kirk, C J, Michell, R H, Biochemical Journal, The inositol phosphates in WRK1 rat mammary tumour cells, Cell Biology, Molecular Biology, Biochemistry |
title | The inositol phosphates in WRK1 rat mammary tumour cells |
title_full | The inositol phosphates in WRK1 rat mammary tumour cells |
title_fullStr | The inositol phosphates in WRK1 rat mammary tumour cells |
title_full_unstemmed | The inositol phosphates in WRK1 rat mammary tumour cells |
title_short | The inositol phosphates in WRK1 rat mammary tumour cells |
title_sort | the inositol phosphates in wrk1 rat mammary tumour cells |
title_unstemmed | The inositol phosphates in WRK1 rat mammary tumour cells |
topic | Cell Biology, Molecular Biology, Biochemistry |
url | http://dx.doi.org/10.1042/bj2860459 |