Eintrag weiter verarbeiten
Rates of ketone-body formation in the perfused rat liver
Gespeichert in:
| Zeitschriftentitel: | Biochemical Journal |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Biochemical Journal, 112, 1969, 5, S. 595-600 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
|
| Schlagwörter: |
| author_facet |
Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. |
|---|---|
| author |
Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. |
| spellingShingle |
Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. Biochemical Journal Rates of ketone-body formation in the perfused rat liver General Medicine |
| author_sort |
krebs, h. a. |
| spelling |
Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. 0306-3283 Portland Press Ltd. General Medicine http://dx.doi.org/10.1042/bj1120595 <jats:p>1. The rates of formation of acetoacetate and β-hydroxybutyrate by the isolated perfused rat liver were measured under various conditions. 2. The rates found after addition of butyrate, octanoate, oleate and linoleate were about 100μmoles/hr./g. wet wt. in the liver of starved rats. These rates are much higher than those found with rat liver slices. 3. The differences between the rates given by slices and by the perfused organ were much higher with the long-chain than with short-chain fatty acids. The increments caused by oleate and linoleate were 12 and 16 times as large in the perfused organ as in the slices, whereas the increments caused by butyrate and octanoate were about four times as large. 4. The rates of ketogenesis in the unsupplemented perfused liver of well-fed rats, and the increments caused by the addition of fatty acids, were about half of those in the liver from starved rats. 5. The value of the [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was raised by octanoate, oleate and linoleate. 6. Carnitine did not significantly accelerate ketogenesis from fatty acids. 7. Oleate formed up to 82% of the expected yield of ketone bodies. 8. In the liver of alloxan-diabetic rats the endogenous rates of ketogenesis were raised, in some cases as high as in the liver from starved rats, after addition of oleate. 9. On addition of either β-hydroxybutyrate or acetoacetate to the perfusion medium the liver gradually adjusted the [β-hydroxybutyrate]/[acetoacetate] ratio towards the normal range. 10. The [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was about 0·4 when slices were incubated, but near the physiological value of 2 when the liver was perfused. 11. The experiments demonstrate that for the study of ketogenesis slices are in many ways grossly inferior to the perfused liver.</jats:p> Rates of ketone-body formation in the perfused rat liver Biochemical Journal |
| doi_str_mv |
10.1042/bj1120595 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajExMjA1OTU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajExMjA1OTU |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Portland Press Ltd., 1969 |
| imprint_str_mv |
Portland Press Ltd., 1969 |
| issn |
0306-3283 |
| issn_str_mv |
0306-3283 |
| language |
English |
| mega_collection |
Portland Press Ltd. (CrossRef) |
| match_str |
krebs1969ratesofketonebodyformationintheperfusedratliver |
| publishDateSort |
1969 |
| publisher |
Portland Press Ltd. |
| recordtype |
ai |
| record_format |
ai |
| series |
Biochemical Journal |
| source_id |
49 |
| title |
Rates of ketone-body formation in the perfused rat liver |
| title_unstemmed |
Rates of ketone-body formation in the perfused rat liver |
| title_full |
Rates of ketone-body formation in the perfused rat liver |
| title_fullStr |
Rates of ketone-body formation in the perfused rat liver |
| title_full_unstemmed |
Rates of ketone-body formation in the perfused rat liver |
| title_short |
Rates of ketone-body formation in the perfused rat liver |
| title_sort |
rates of ketone-body formation in the perfused rat liver |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.1042/bj1120595 |
| publishDate |
1969 |
| physical |
595-600 |
| description |
<jats:p>1. The rates of formation of acetoacetate and β-hydroxybutyrate by the isolated perfused rat liver were measured under various conditions. 2. The rates found after addition of butyrate, octanoate, oleate and linoleate were about 100μmoles/hr./g. wet wt. in the liver of starved rats. These rates are much higher than those found with rat liver slices. 3. The differences between the rates given by slices and by the perfused organ were much higher with the long-chain than with short-chain fatty acids. The increments caused by oleate and linoleate were 12 and 16 times as large in the perfused organ as in the slices, whereas the increments caused by butyrate and octanoate were about four times as large. 4. The rates of ketogenesis in the unsupplemented perfused liver of well-fed rats, and the increments caused by the addition of fatty acids, were about half of those in the liver from starved rats. 5. The value of the [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was raised by octanoate, oleate and linoleate. 6. Carnitine did not significantly accelerate ketogenesis from fatty acids. 7. Oleate formed up to 82% of the expected yield of ketone bodies. 8. In the liver of alloxan-diabetic rats the endogenous rates of ketogenesis were raised, in some cases as high as in the liver from starved rats, after addition of oleate. 9. On addition of either β-hydroxybutyrate or acetoacetate to the perfusion medium the liver gradually adjusted the [β-hydroxybutyrate]/[acetoacetate] ratio towards the normal range. 10. The [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was about 0·4 when slices were incubated, but near the physiological value of 2 when the liver was perfused. 11. The experiments demonstrate that for the study of ketogenesis slices are in many ways grossly inferior to the perfused liver.</jats:p> |
| container_issue |
5 |
| container_start_page |
595 |
| container_title |
Biochemical Journal |
| container_volume |
112 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792337877963636752 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:23:08.596Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Rates+of+ketone-body+formation+in+the+perfused+rat+liver&rft.date=1969-05-01&genre=article&issn=0306-3283&volume=112&issue=5&spage=595&epage=600&pages=595-600&jtitle=Biochemical+Journal&atitle=Rates+of+ketone-body+formation+in+the+perfused+rat+liver&aulast=Freedland&aufirst=R.+A.&rft_id=info%3Adoi%2F10.1042%2Fbj1120595&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792337877963636752 |
| author | Krebs, H. A., Wallace, Patricia G., Hems, R., Freedland, R. A. |
| author_facet | Krebs, H. A., Wallace, Patricia G., Hems, R., Freedland, R. A., Krebs, H. A., Wallace, Patricia G., Hems, R., Freedland, R. A. |
| author_sort | krebs, h. a. |
| container_issue | 5 |
| container_start_page | 595 |
| container_title | Biochemical Journal |
| container_volume | 112 |
| description | <jats:p>1. The rates of formation of acetoacetate and β-hydroxybutyrate by the isolated perfused rat liver were measured under various conditions. 2. The rates found after addition of butyrate, octanoate, oleate and linoleate were about 100μmoles/hr./g. wet wt. in the liver of starved rats. These rates are much higher than those found with rat liver slices. 3. The differences between the rates given by slices and by the perfused organ were much higher with the long-chain than with short-chain fatty acids. The increments caused by oleate and linoleate were 12 and 16 times as large in the perfused organ as in the slices, whereas the increments caused by butyrate and octanoate were about four times as large. 4. The rates of ketogenesis in the unsupplemented perfused liver of well-fed rats, and the increments caused by the addition of fatty acids, were about half of those in the liver from starved rats. 5. The value of the [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was raised by octanoate, oleate and linoleate. 6. Carnitine did not significantly accelerate ketogenesis from fatty acids. 7. Oleate formed up to 82% of the expected yield of ketone bodies. 8. In the liver of alloxan-diabetic rats the endogenous rates of ketogenesis were raised, in some cases as high as in the liver from starved rats, after addition of oleate. 9. On addition of either β-hydroxybutyrate or acetoacetate to the perfusion medium the liver gradually adjusted the [β-hydroxybutyrate]/[acetoacetate] ratio towards the normal range. 10. The [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was about 0·4 when slices were incubated, but near the physiological value of 2 when the liver was perfused. 11. The experiments demonstrate that for the study of ketogenesis slices are in many ways grossly inferior to the perfused liver.</jats:p> |
| doi_str_mv | 10.1042/bj1120595 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajExMjA1OTU |
| imprint | Portland Press Ltd., 1969 |
| imprint_str_mv | Portland Press Ltd., 1969 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0306-3283 |
| issn_str_mv | 0306-3283 |
| language | English |
| last_indexed | 2024-03-01T15:23:08.596Z |
| match_str | krebs1969ratesofketonebodyformationintheperfusedratliver |
| mega_collection | Portland Press Ltd. (CrossRef) |
| physical | 595-600 |
| publishDate | 1969 |
| publishDateSort | 1969 |
| publisher | Portland Press Ltd. |
| record_format | ai |
| recordtype | ai |
| series | Biochemical Journal |
| source_id | 49 |
| spelling | Krebs, H. A. Wallace, Patricia G. Hems, R. Freedland, R. A. 0306-3283 Portland Press Ltd. General Medicine http://dx.doi.org/10.1042/bj1120595 <jats:p>1. The rates of formation of acetoacetate and β-hydroxybutyrate by the isolated perfused rat liver were measured under various conditions. 2. The rates found after addition of butyrate, octanoate, oleate and linoleate were about 100μmoles/hr./g. wet wt. in the liver of starved rats. These rates are much higher than those found with rat liver slices. 3. The differences between the rates given by slices and by the perfused organ were much higher with the long-chain than with short-chain fatty acids. The increments caused by oleate and linoleate were 12 and 16 times as large in the perfused organ as in the slices, whereas the increments caused by butyrate and octanoate were about four times as large. 4. The rates of ketogenesis in the unsupplemented perfused liver of well-fed rats, and the increments caused by the addition of fatty acids, were about half of those in the liver from starved rats. 5. The value of the [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was raised by octanoate, oleate and linoleate. 6. Carnitine did not significantly accelerate ketogenesis from fatty acids. 7. Oleate formed up to 82% of the expected yield of ketone bodies. 8. In the liver of alloxan-diabetic rats the endogenous rates of ketogenesis were raised, in some cases as high as in the liver from starved rats, after addition of oleate. 9. On addition of either β-hydroxybutyrate or acetoacetate to the perfusion medium the liver gradually adjusted the [β-hydroxybutyrate]/[acetoacetate] ratio towards the normal range. 10. The [β-hydroxybutyrate]/[acetoacetate] ratio of the medium was about 0·4 when slices were incubated, but near the physiological value of 2 when the liver was perfused. 11. The experiments demonstrate that for the study of ketogenesis slices are in many ways grossly inferior to the perfused liver.</jats:p> Rates of ketone-body formation in the perfused rat liver Biochemical Journal |
| spellingShingle | Krebs, H. A., Wallace, Patricia G., Hems, R., Freedland, R. A., Biochemical Journal, Rates of ketone-body formation in the perfused rat liver, General Medicine |
| title | Rates of ketone-body formation in the perfused rat liver |
| title_full | Rates of ketone-body formation in the perfused rat liver |
| title_fullStr | Rates of ketone-body formation in the perfused rat liver |
| title_full_unstemmed | Rates of ketone-body formation in the perfused rat liver |
| title_short | Rates of ketone-body formation in the perfused rat liver |
| title_sort | rates of ketone-body formation in the perfused rat liver |
| title_unstemmed | Rates of ketone-body formation in the perfused rat liver |
| topic | General Medicine |
| url | http://dx.doi.org/10.1042/bj1120595 |